LOCUS       sequence-32061-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Beronja S, Livshits G, Williams S, Fuchs E
  TITLE     Rapid functional dissection of genetic networks via tissue-specific 
            transduction and RNAi in mouse embryos.
  JOURNAL   Nat Med. 2010 Jul . 16(7):821-7.
  PUBMED    20526348
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med. 
            2010 Jul . 16(7):821-7."
COMMENT     Sequence Label: LV-YFP
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             88..321
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(395..646)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(437..458)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     786..805
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctgcattaaa caagttcctc tcactctctg atattcattt ctttgcaagt tataaatact
       61 gaataataag atgacatgaa ctactactgc tagagatttt ccacactgac taaaagggtc
      121 tgagggatct ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc
      181 aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg
      241 ctcagatctg gtctaaccag agagacccag tacaagcaaa aagcagatct tgtcttcgtt
      301 gggagtgaat tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag
      361 ctgccttgta agtcattggt cttaaaggta cctacttgta cagctcgtcc atgccgagag
      421 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
      481 ctttgctcag ggcggactgg tagctcaggt agtggttgtc gggcagcagc acggggccgt
      541 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt
      601 tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga
      661 cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga
      721 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
      781 cgcgggtctt gtagttgccg tcgtccttga agaa
//
