LOCUS       sequence-320614-        7215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  W1.1 plasmid. Contains PTetO-sd2U-Cas9, PLacO-sgRNA1 and is part of 
            CAMERA 1.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Tang W, Liu DR
  TITLE     Rewritable multi-event analog recording in bacterial and mammalian 
            cells.
  JOURNAL   Science. 2018 Feb 15. pii: science.aap8992. doi: 
            10.1126/science.aap8992.
  PUBMED    29449507
REFERENCE   2  (bases 1 to 7215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992."
COMMENT     Sequence Label: pWT019a
FEATURES             Location/Qualifiers
     source          1..7215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(23..973)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTVKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      1021..1243
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      1738..1784
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    1818..1836
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        1837..1891
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    1842..1860
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    1872..1890
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             1948..6051
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1948..6048
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1948..6048
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_RNA        complement(6165..6240)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    6273..6289
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(6347..7138)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(7139..7210)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agaaaaatag gccaaatgcc gttcagatcc ttccgtattt agccagtatg ttctctagtg
       61 tggttcgttg tttttgcgtg agccatgaga acgaaccatt gagatcatgc ttactttgca
      121 tgtcactcaa aaattttgcc tcaaaactgg tgagctgaat ttttacagtt aaagcatcgt
      181 gtagtgtttt tcttagtccg ttacgtaggt aggaatctga tgtaatggtt gttggtattt
      241 tgtcaccatt catttttatc tggttgttct caagttcggt tacgagatcc atttgtctat
      301 ctagttcaac ttggaaaatc aacgtatcag tcgggcggcc tcgcttatca accaccaatt
      361 tcatattgct gtaagtgttt aaatctttac ttattggttt caaaacccat tggttaagcc
      421 ttttaaactc atggtagtta ttttcaagca ttaacatgaa cttaaattca tcaaggctaa
      481 tctctatatt tgccttgtga gttttctttt gtgttagttc ttttaataac cactcataaa
      541 tcctcataga gtatttgttt tcaaaagact taacatgttc cagattatat tttatgaatt
      601 tttttaactg gaaaagataa ggcaatatct cttcactaaa aactaattct aatttttcgc
      661 ttgagaactt ggcatagttt gtccactgga aaatctcaaa gcctttaacc aaaggattcc
      721 tgatttccac agttctcgtc atcagctctc tggttgcttt agctaataca ccataagcat
      781 tttccctact gatgttcatc atctgagcgt attggttata agtgaacgat accgtccgtt
      841 ctttccttgt agggttttca atcgtggggt tgagtagtgc cacacagcat aaaattagct
      901 tggtttcatg ctccgttaag tcatagcgac taatcgctag ttcatttgct ttgaaaacaa
      961 ctaattcaga catacatctc aattggtcta ggtgatttta atcactatac caattgagat
     1021 gggctagtca atgataatta ctagtccttt tcctttgagt tgtgggtatc tgtaaattct
     1081 gctagacctt tgctggaaaa cttgtaaatt ctgctagacc ctctgtaaat tccgctagac
     1141 ctttgtgtgt tttttttgtt tatattcaag tggttataat ttatagaata aagaaagaat
     1201 aaaaaaagat aaaaagaata gatcccagcc ctgtgtataa ctcactactt tagtcagttc
     1261 cgcagtatta caaaaggatg tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa
     1321 accctaaagg cttaagtagc accctcgcaa gctcgggcaa atcgctgaat attccttttg
     1381 tctccgacca tcaggcacct gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc
     1441 acggctctgg cagtgaatgg gggtaaatgg cactacaggc gccttttatg gattcatgca
     1501 aggaaactac ccataataca agaaaagccc gtcacgggct tctcagggcg ttttatggcg
     1561 ggtctgctat gtggtgctat ctgacttttt gctgttcagc agttcctgcc ctctgatttt
     1621 ccagtctgac cacttcggat tatcccgtga caggtcattc agactggcta atgcacccag
     1681 taaggcagcg gtatcatcaa caggcttacc cgtcttactg tcaagaggac atccggtcaa
     1741 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttattctaga gcacagctcc
     1801 atcgaatggc cagatgatcc ctatcagtga tagagattga cactctatca ttgatagagt
     1861 tattttacca ctccctatca gtgatagaga aaagtgaaat gaatagttcg acaaaaatct
     1921 agacctgcag gtgcagtaag gaaattggac aagaagtact ccattgggct cgatatcggc
     1981 acaaacagcg tcggctgggc cgtcattacg gacgagtaca aggtgccgag caaaaaattc
     2041 aaagttctgg gcaataccga tcgccacagc ataaagaaga acctcattgg cgccctcctg
     2101 ttcgactccg gggagacggc cgaagccacg cggctcaaaa gaacagcacg gcgcagatat
     2161 acccgcagaa agaatcggat ctgctacctg caggagatct ttagtaatga gatggctaag
     2221 gtggatgact ctttcttcca taggctggag gagtcctttt tggtggagga ggataaaaag
     2281 cacgagcgcc acccaatctt tggcaatatc gtggacgagg tggcgtacca tgaaaagtac
     2341 ccaaccatat atcatctgag gaagaagctt gtagacagta ctgataaggc tgacttgcgg
     2401 ttgatctatc tcgcgctggc gcatatgatc aaatttcggg gacacttcct catcgagggg
     2461 gacctgaacc cagacaacag cgatgtcgac aaactcttta tccaactggt tcagacttac
     2521 aatcagcttt tcgaagagaa cccgatcaac gcatccggag ttgacgccaa agcaatcctg
     2581 agcgctaggc tgtccaaatc ccggcggctc gaaaacctca tcgcacagct ccctggggag
     2641 aagaagaacg gcctgtttgg taatcttatc gccctgtcac tcgggctgac ccccaacttt
     2701 aaatctaact tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga cacctacgat
     2761 gatgatctcg acaatctgct ggcccagatc ggcgaccagt acgcagacct ttttttggcg
     2821 gcaaagaacc tgtcagacgc cattctgctg agtgatattc tgcgagtgaa cacggagatc
     2881 accaaagctc cgctgagcgc tagtatgatc aagcgctatg atgagcacca ccaagacttg
     2941 actttgctga aggcccttgt cagacagcaa ctgcctgaga agtacaagga aattttcttc
     3001 gatcagtcta aaaatggcta cgccggatac attgacggcg gagcaagcca ggaggaattt
     3061 tacaaattta ttaagcccat cttggaaaaa atggacggca ccgaggagct gctggtaaag
     3121 cttaacagag aagatctgtt gcgcaaacaa cgcactttcg acaatggaag catcccccac
     3181 cagattcacc tgggcgaact gcacgctatc ctcaggcggc aagaggattt ctaccccttt
     3241 ttgaaagata acagggaaaa gattgagaaa atcctcacat ttcggatacc ctactatgta
     3301 ggccccctcg cccggggaaa ttccagattc gcgtggatga ctcgcaaatc agaagagacc
     3361 atcactccct ggaacttcga ggaagtcgtg gataaggggg cctctgccca gtccttcatc
     3421 gaaaggatga ctaactttga taaaaatctg cctaacgaaa aggtgcttcc taaacactct
     3481 ctgctgtacg agtacttcac agtttataac gagctcacca aggtcaaata cgtcacagaa
     3541 gggatgagaa agccagcatt cctgtctgga gagcagaaga aagctatcgt ggacctcctc
     3601 ttcaagacga accggaaagt taccgtgaaa cagctcaaag aagactattt caaaaagatt
     3661 gaatgtttcg actctgttga aatcagcgga gtggaggatc gcttcaacgc atccctggga
     3721 acgtatcacg atctcctgaa aatcattaaa gacaaggact tcctggacaa tgaggagaac
     3781 gaggacattc ttgaggacat tgtcctcacc cttacgttgt ttgaagatag ggagatgatt
     3841 gaagaacgct tgaaaactta cgctcatctc ttcgacgaca aagtcatgaa acagctcaag
     3901 aggcgccgat atacaggatg ggggcggctg tcaagaaaac tgatcaatgg gatccgagac
     3961 aagcagagtg gaaagacaat cctggatttt cttaagtccg atggatttgc caaccggaac
     4021 ttcatgcagt tgatccatga tgactctctc acctttaagg aggacatcca gaaagcacaa
     4081 gtttctggcc agggggacag tcttcacgag cacatcgcta atcttgcagg tagcccagct
     4141 atcaaaaagg gaatactgca gaccgttaag gtcgtggatg aactcgtcaa agtaatggga
     4201 aggcataagc ccgagaatat cgttatcgag atggcccgag agaaccaaac tacccagaag
     4261 ggacagaaga acagtaggga aaggatgaag aggattgaag agggtataaa agaactgggg
     4321 tcccaaatcc ttaaggaaca cccagttgaa aacacccagc ttcagaatga gaagctctac
     4381 ctgtactacc tgcagaacgg cagggacatg tacgtggatc aggaactgga catcaatcgg
     4441 ctctccgact acgacgtgga ccatatcgtg ccccagtctt ttctcaaaga tgattctatt
     4501 gataataaag tgttgacaag atccgataaa aacagaggga agagtgataa cgtcccctca
     4561 gaagaagttg tcaagaaaat gaaaaattat tggcggcagc tgctgaacgc caaactgatc
     4621 acacaacgga agttcgataa tctgactaag gctgaacgag gtggcctgtc tgagttggat
     4681 aaagccggct tcatcaaaag gcagcttgtt gagacacgcc agatcaccaa gcacgtggcc
     4741 caaattctcg attcacgcat gaacaccaag tacgatgaaa atgacaaact gattcgagag
     4801 gtgaaagtta ttactctgaa gtctaagctg gtctcagatt tcagaaagga ctttcagttt
     4861 tataaggtga gagagatcaa caattaccac catgcgcatg atgcctacct gaatgcagtg
     4921 gtaggcactg cacttatcaa aaaatatccc aagcttgaat ctgaatttgt ttacggagac
     4981 tataaagtgt acgatgttag gaaaatgatc gcaaagtctg agcaggaaat aggcaaggcc
     5041 accgctaagt acttctttta cagcaatatt atgaattttt tcaagaccga gattacactg
     5101 gccaatggag agattcggaa gcgaccactt atcgaaacaa acggagaaac aggagaaatc
     5161 gtgtgggaca agggtaggga tttcgcgaca gtccggaagg tcctgtccat gccgcaggtg
     5221 aacatcgtta aaaagaccga agtacagacc ggaggcttct ccaaggaaag tatcctcccg
     5281 aaaaggaaca gcgacaagct gatcgcacgc aaaaaagatt gggaccccaa gaaatacggc
     5341 ggattcgatt ctcctacagt cgcttacagt gtactggttg tggccaaagt ggagaaaggg
     5401 aagtctaaaa aactcaaaag cgtcaaggaa ctgctgggca tcacaatcat ggagcgatca
     5461 agcttcgaaa aaaaccccat cgactttctc gaggcgaaag gatataaaga ggtcaaaaaa
     5521 gacctcatca ttaagcttcc caagtactct ctctttgagc ttgaaaacgg ccggaaacga
     5581 atgctcgcta gtgcgggcga gctgcagaaa ggtaacgagc tggcactgcc ctctaaatac
     5641 gttaatttct tgtatctggc cagccactat gaaaagctca aagggtctcc cgaagataat
     5701 gagcagaagc agctgttcgt ggaacaacac aaacactacc ttgatgagat catcgagcaa
     5761 ataagcgaat tctccaaaag agtgatcctc gccgacgcta acctcgataa ggtgctttct
     5821 gcttacaata agcacaggga taagcccatc agggagcagg cagaaaacat tatccacttg
     5881 tttactctga ccaacttggg cgcgcctgca gccttcaagt acttcgacac caccatagac
     5941 agaaagcggt acacctctac aaaggaggtc ctggacgcca cactgattca tcagtcaatt
     6001 acggggctct atgaaacaag aatcgacctc tctcagctcg gtggagactg aacggagcca
     6061 atgtacgcaa aaaccccgct tcagcggggt tttttcgcca aaaaaaaccc cgccctgtca
     6121 ggggcggggt ttttttttat gttgaaaatc tccttctaaa aaaagcaccg actcggtgcc
     6181 actttttcaa gttgataacg gactagcctt attttaactt gctatttcta gctctaaaac
     6241 ccggcaagct gcccgtgccc gtgctcagta tcttgttatc cgctcacaat taaatataat
     6301 gaccctcttg ataacccaag agggcatttt ttaatgccca tggcgtttat ttgccgacta
     6361 ccttggtgat ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg
     6421 ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat
     6481 actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc
     6541 cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag
     6601 cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct
     6661 gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt
     6721 ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac
     6781 ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac
     6841 gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc
     6901 tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt
     6961 catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc
     7021 catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga
     7081 tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcatac
     7141 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     7201 tatttaaatg tattt
//
