LOCUS       sequence-320615-        9843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Extracellular domain of SARS-CoV-2 spike protein that has been 
            engineered so that it assembles pseudo virus more efficiently. Also 
            contains portions of VSV-G for production of psuedovirus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9843)
  AUTHORS   Tong J, Zhu C, Lai H, Feng C, Zhou D
  TITLE     Potent Neutralization Antibodies Induced by a Recombinant Trimeric 
            Spike Protein Vaccine Candidate Containing PIKA Adjuvant for 
            COVID-19.
  JOURNAL   Vaccines (Basel). 2021 Mar 22;9(3). pii: vaccines9030296. doi: 
            10.3390/vaccines9030296.
  PUBMED    33810026
REFERENCE   2  (bases 1 to 9843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Vaccines 
            (Basel)."; date: "2021-03-22"; volume: "9(3). pii: vaccines9030296. 
            doi"; pages: " 10.3390/vaccines9030296"
COMMENT     Sequence Label: Zhou-COVID-19-Spike
FEATURES             Location/Qualifiers
     source          1..9843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        322..525
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     476..496
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     522..546
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1027..1046
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1075..1094
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1075..1093
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(2446..2463)
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             4872..4904
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     primer_bind     complement(4954..4971)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4960..5184
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5230..5658
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5317..5336)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5527..5548
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5667..5687)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5672..6001
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5852..5987
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5914..5933
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6050..7075
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6050..7075
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    7205..7326
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7242..7261)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7296..7315
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7375..7391)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7375..7391)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7388..7410)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7399..7415
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7423..7429,7430..7447,7448..7453))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7468..7489
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7606..7623)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7777..8365)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7857..7876)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8536..9327,9328..9396))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9159..9178
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9397..9501)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9576..9595)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9785..9843,1..321)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
       61 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      121 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      181 caagtgtatc atatgccaag tccgccccct attgacgtca atgacggtaa atggcccgcc
      241 tggcattatg cccagtacat gaccttacgg gactttccta cttggcagta catctacgta
      301 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag
      361 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      421 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa
      481 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
      541 cagatcctca ctctcttccg catcgctgtc tgcgagggcc agctgttggg ctcgcggttg
      601 aggacaaact cttcgcggtc tttccagtac tcttggatcg gaaacccgtc ggcctccgaa
      661 cggtactccg ccaccgaggg acctgagcga gtccgcatcg accggatcgg aaaacctctc
      721 gagaaaggcg tctaaccagt cacagtcgca aggtaggctg agcaccgtgg cgggcggcag
      781 cgggtggcgg tcggggttgt ttctggcgga ggtgctgctg atgatgtaat taaagtaggc
      841 ggtcttgaga cggcggatgg tcgaggtgag gtgtggcagg cttgagatcc agctgttggg
      901 gtgagtactc cctctcaaaa gcgggcatta cttctgcgct aagattgtca gtttccaaaa
      961 acgaggagga tttgatattc acctggcccg atctggccat acacttgagt gacaatgaca
     1021 tccactttgc ctttctctcc acaggtgtcc actcccaggt ccaagtttaa actttaatac
     1081 gactcactat aggggccgcc accaagcttg gtaccgccgc caccatgaaa tgtcttctct
     1141 atttggcgtt cctgtttatc ggagtgaact gtcagtgtgt gaacctgacc accaggaccc
     1201 aacttcctcc tgcctacacc aactccttca ccaggggagt ctactaccct gacaaggtgt
     1261 tcaggtcctc tgtgctgcac agcacccagg acctgttcct gccattcttc agcaatgtga
     1321 cctggttcca tgccatccat gtgtctggca ccaatggcac caagaggttt gacaaccctg
     1381 tgctgccatt caatgatgga gtctactttg ccagcacaga gaagagcaac atcatcaggg
     1441 gctggatttt tggcaccacc ctggacagca agacccagtc cctgctgatt gtgaacaatg
     1501 ccaccaatgt ggtgattaag gtgtgtgagt tccagttctg taatgaccca ttcctgggag
     1561 tctactacca caagaacaac aagtcctgga tggagtctga gttcagggtc tactcctctg
     1621 ccaacaactg tacctttgaa tatgtgagcc aaccattcct gatggacttg gagggcaagc
     1681 agggcaactt caagaacctg agggagtttg tgttcaagaa cattgatggc tacttcaaga
     1741 tttacagcaa acacacacca atcaacctgg tgagggacct gccacagggc ttctctgcct
     1801 tggaaccact ggtggacctg ccaattggca tcaacatcac caggttccag accctgctgg
     1861 ctctgcacag gtcctacctg acacctggag actcctcctc tggctggaca gcaggagcag
     1921 cagcctacta tgtgggctac ctccaaccaa ggaccttcct gctgaaatac aatgagaatg
     1981 gcaccatcac agatgctgtg gactgtgccc tggacccact gtctgagacc aagtgtaccc
     2041 tgaaatcctt cacagtggag aagggcatct accagaccag caacttcagg gtccaaccaa
     2101 cagagagcat tgtgaggttt ccaaacatca ccaacctgtg tccatttgga gaggtgttca
     2161 atgccaccag gtttgcctct gtctatgcct ggaacaggaa gaggattagc aactgtgtgg
     2221 ctgactactc tgtgctctac aactctgcct ccttcagcac cttcaagtgt tatggagtga
     2281 gcccaaccaa actgaatgac ctgtgtttca ccaatgtcta tgctgactcc tttgtgatta
     2341 ggggagatga ggtgagacag attgcccctg gacaaacagg caagattgct gactacaact
     2401 acaaactgcc tgatgacttc acaggctgtg tgattgcctg gaacagcaac aacctggaca
     2461 gcaaggtggg aggcaactac aactacctct acagactgtt caggaagagc aacctgaaac
     2521 catttgagag ggacatcagc acagagattt accaggctgg cagcacacca tgtaatggag
     2581 tggagggctt caactgttac tttccactcc aatcctatgg cttccaacca accaatggag
     2641 tgggctacca accatacagg gtggtggtgc tgtcctttga actgctccat gcccctgcca
     2701 cagtgtgtgg accaaagaag agcaccaacc tggtgaagaa caagtgtgtg aacttcaact
     2761 tcaatggact gacaggcaca ggagtgctga cagagagcaa caagaagttc ctgccattcc
     2821 aacagtttgg cagggacatt gctgacacca cagatgctgt gagggaccca cagaccttgg
     2881 agattctgga catcacacca tgttcctttg gaggagtgtc tgtgattaca cctggcacca
     2941 acaccagcaa ccaggtggct gtgctctacc aggatgtgaa ctgtactgag gtgcctgtgg
     3001 ctatccatgc tgaccaactt acaccaacct ggagggtcta cagcacaggc agcaatgtgt
     3061 tccagaccag ggctggctgt ctgattggag cagagcatgt gaacaactcc tatgagtgtg
     3121 acatcccaat tggagcaggc atctgtgcct cctaccagac ccagaccaac agcccaagga
     3181 gggcaaggtc tgtggcaagc cagagcatca ttgcctacac aatgagtctg ggagcagaga
     3241 actctgtggc ttacagcaac aacagcattg ccatcccaac caacttcacc atctctgtga
     3301 ccacagagat tctgcctgtg agtatgacca agacctctgt ggactgtaca atgtatatct
     3361 gtggagacag cacagagtgt agcaacctgc tgctccaata tggctccttc tgtacccaac
     3421 ttaacagggc tctgacaggc attgctgtgg aacaggacaa gaacacccag gaggtgtttg
     3481 cccaggtgaa gcagatttac aagacacctc caatcaagga ctttggaggc ttcaacttca
     3541 gccagattct gcctgaccca agcaagccaa gcaagaggtc cttcattgag gacctgctgt
     3601 tcaacaaggt gaccctggct gatgctggct tcatcaagca atatggagac tgtctgggag
     3661 acattgctgc cagggacctg atttgtgccc agaagttcaa tggactgaca gtgctgcctc
     3721 cactgctgac agatgagatg attgcccaat acacctctgc cctgctggct ggcaccatca
     3781 cctctggctg gacctttgga gcaggagcag ccctccaaat cccatttgct atgcagatgg
     3841 cttacaggtt caatggcatt ggagtgaccc agaatgtgct ctatgagaac cagaaactga
     3901 ttgccaacca gttcaactct gccattggca agattcagga ctccctgtcc agcacagcct
     3961 ctgccctggg caaactccaa gatgtggtga accagaatgc ccaggctctg aacaccctgg
     4021 tgaagcaact ttccagcaac tttggagcca tctcctctgt gctgaatgac atcctgagca
     4081 gactggacaa ggtggaggct gaggtccaga ttgacagact gattacaggc agactccaat
     4141 ccctccaaac ctatgtgacc caacaactta tcagggctgc tgagattagg gcatctgcca
     4201 acctggctgc caccaagatg agtgagtgtg tgctgggaca aagcaagagg gtggacttct
     4261 gtggcaaggg ctaccacctg atgagttttc cacagtctgc ccctcatgga gtggtgttcc
     4321 tgcatgtgac ctatgtgcct gcccaggaga agaacttcac cacagcccct gccatctgcc
     4381 atgatggcaa ggctcacttt ccaagggagg gagtgtttgt gagcaatggc acccactggt
     4441 ttgtgaccca gaggaacttc tatgaaccac agattatcac cacagacaac acctttgtgt
     4501 ctggcaactg tgatgtggtg attggcattg tgaacaacac agtctatgac ccactccaac
     4561 ctgaactgga ctccttcaag gaggaactgg acaaatactt caagaaccac accagccctg
     4621 atgtggacct gggagacatc tctggcatca atgcctctgt ggtgaacatc cagaaggaga
     4681 ttgacagact gaatgaggtg gctaagaacc tgaatgagtc cctgattgac ctccaagaac
     4741 tgggcaaata tgaacaatac atcaagtggc catttttctt cattattggg ctcatcatcg
     4801 gcctgtttct ggtgttgagg gtgggaatcc acctctgcat caaactgaag cacacaaaaa
     4861 aaagacagat ctatacagac atcgagatga accggctggg caagtaatct agagcggccg
     4921 ccgaattcgg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     4981 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     5041 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     5101 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     5161 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     5221 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     5281 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     5341 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     5401 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     5461 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     5521 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     5581 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     5641 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     5701 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     5761 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5821 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     5881 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     5941 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     6001 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtga tgaaaaagcc
     6061 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga
     6121 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
     6181 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
     6241 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag
     6301 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc
     6361 tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc
     6421 ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata
     6481 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac
     6541 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg
     6601 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
     6661 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga
     6721 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca
     6781 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc
     6841 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga
     6901 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt
     6961 cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt
     7021 actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagcacgt
     7081 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     7141 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     7201 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7261 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7321 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     7381 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7441 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7501 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7561 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7621 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7681 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7741 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7801 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7861 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7921 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7981 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8041 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8101 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8161 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8221 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8281 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8341 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8401 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8461 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8521 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8581 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8641 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8701 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8761 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8821 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8881 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8941 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9001 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9061 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9121 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9181 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9241 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9301 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9361 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9421 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9481 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     9541 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     9601 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     9661 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     9721 aggcgttttg cgctgcttcg cgagtacatt tatattggct catgtccaat atgaccgcca
     9781 tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     9841 agc
//
