LOCUS       sequence-320623-        9117 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR repair template: add insertion site specific homology arms by
            PCR before insertion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9117)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 9117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pJW1927
FEATURES             Location/Qualifiers
     source          1..9117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     95..112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(271..298)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(390..476)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     525..547
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     539..556
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     540..556
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1448..1481
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             1491..1520
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3337..3660)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5578..5602
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5605..6630
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5605..6630
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6631..6655)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    7008..7041
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             7066..7086
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             7108..7239
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             7267..7332
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(7361..7380)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7362..7380)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7385..7401)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7385..7401)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7503..7522)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7514..8323
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7581..7600)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      8470..9058
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8959..8978
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
       61 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      121 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      181 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac
      241 cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc
      301 ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc
      361 ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac
      421 aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc
      481 ctggcagttc cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg
      541 taaaacgacg gccagtctta agctcgggcc ccaaataatg ctcttcgatg gtctccaaag
      601 gagaagctgt gatcaaggag ttcatgcgct tcaaggttca catggaagga agcatgaatg
      661 gtcacgagtt cgaaatcgaa ggagaagggg agggccgccc gtacgaggga actcagaccg
      721 ccaagctgaa ggtcaccaag ggaggtaagt ttgtgataat ccaatttcaa ttcgcaatgg
      781 tcatcgtttt ttcaggacca cttccattct catgggatat tctctcccca caattcatgt
      841 acggctcccg tgctttcatc aaacacccag ccgacattcc agattactac aagcaatctt
      901 tcccagaagg cttcaagtgg gagcgtgtga tgaacttcga ggatggtgga gcagttacag
      961 taactcagga cacttctctc gaggtaagtt tttactccgc ttttaacaat ggttgtttga
     1021 catcattttt tcaggatggc actttgatct acaaggtcaa gctccgtggt accaatttcc
     1081 caccagatgg accagttatg cagaagaaga cgatgggatg ggaggcttcc accgaacgat
     1141 tgtacccaga agatggagtt ctcaagggag acatcaaaat ggcgttacgc ctcaaggacg
     1201 gaggtaagtt tgatgaaacg gtttcgtctt atatacacta atggtacttt tcaggacgtt
     1261 acctggcgga tttcaagacc acatacaagg caaagaagcc agttcaaatg ccaggagcat
     1321 ataacgttga ccgcaagctt gatattactt cccataatga agactacaca gttgtagaac
     1381 aatacgaacg atccgaggga cgtcattcga ccggaggaat ggacgagctg tacaaggtaa
     1441 gtttaaaata acttcgtata atgtatacta tacgaagtta ttttcaggga gagcaaaagc
     1501 ttatctccga ggaggacctc taaggatgat cgacgccaac gtcgttgaat tttcaaattt
     1561 taaatactga atatttgttt tttttcctat tatttattta ttctctttgt gttttttttc
     1621 ttgctttcta aaaaattaat tcaatccaaa tctaaacatt tttttttctc tttccgtctc
     1681 ccaattcgta ttccgctcct ctcatctgaa cacaatgtgc aagtttattt atcttctcgc
     1741 tttcatttca ttaggacgtg gggggaattg gtggaagggg gaaacacaca aaaggatgat
     1801 ggaaatgaaa taaggacaca caatatgcaa caacattcaa ttcagaaata tggaggaagg
     1861 tttaaaagaa aacataaaaa tatatagagg aggaaggaaa ctagctgccc tcgattgtct
     1921 atcttccatt ctcaccaagt ttcaacaact ttattcagtc attcttctct ggttcatttc
     1981 ccaaccaatc ttcccgaata aagcccatct gtcctttcgg ccacattcct cacctacatc
     2041 cgtactcctt atctcccgtt cttatccagt cttttatcgt ctctgcgtct cgcacactgt
     2101 ctcgtcggta atgtaaaaaa aaaacaaaaa aaacttgaaa acttgcggaa agatttccta
     2161 ataaaaggag acgaatccgg taggaaatcg acaagtctct gcttctcgaa gactaggtga
     2221 gttacatttc tttggccgaa ttattttgaa atcctaaatt tgataatttc agagatgtct
     2281 gtaaaacttg cgtgttatgt gacggcttcg gtcactgtcg ccactcttat ggtatgcttc
     2341 atgaccatgt caaccatcta ctcggaggtt gatggattca gagagaagct tgataccgag
     2401 atgaatgtgt tcagagtgag ttaaattata ttttgaattt taaataattt ttaattttct
     2461 agcaatctac caatggattg tggaaggaca tagttgtcat cggaagatct agcaagcgtg
     2521 tccgttgtca atatgaagag accaacgcta ccccaactcc acatgctgat ggatccccat
     2581 ctgctccacc aggtcaacca ccagcagttc caccagtctt caaccagcca aagactccaa
     2641 atggagccaa tggaaatgga ccaacctgca actgcaatgc tgataacaag tgcccagctg
     2701 gaccatccgg accaaaggga gttccaggag ttccaggact cgacggagtt ccaggacttg
     2761 acggtgttcc aggagttgga gctgatgata tcgctccaca acgcgagtct gtcggatgct
     2821 tcacttgccc acaaggacca gttggaccac caggagctct tggaagacca ggaccacgtg
     2881 gacttccagg accaagagga caaaatggaa acccaggaag agatggacaa ccaggacatc
     2941 caggagagca aggatcatcc ggtcaaatcg gaaagatcgg agagccagga ccaccaggag
     3001 agaagggacg cgacgccgag catccaatcg gaagaccagg accaaaggga ccaagaggag
     3061 atcaaggacc aacaggacca gctggacaga acggtcttca cggaccacca ggagagccag
     3121 gaaccgttgg accagaagga ccatctggaa agcaaggacg tcaaggacca gacggaaccc
     3181 agggagagac tggaccagac ggaagaccag gaaaggatgc cgagtactgc cagtgcccag
     3241 acaagtctcc accatcagag gctgtcaacg ccaaccgtgg atacagaaat atctaaattg
     3301 ttggtgtttt ctaataaaat atttgagatt cagtgagact tttttcttgg cggcacaata
     3361 aagtttcctc tttttgttgg gtcactcaac gaacacaata caaatatttg agcgctcgca
     3421 tttttctcaa cctttcgggt ttaaagcact tcaaaatgga attgaagaga gattgtgaaa
     3481 gtacatcaga cagaatcaca ttttgatagc attcacttca ctcagatgca aacttttcag
     3541 tgactataaa aagtcataaa ataataacat taaaaaagca aataaattag cgagagaatt
     3601 ttttgacaaa aagaaagaag agtgatagag aagaagggaa tgcttgaaag gattttgcat
     3661 ttatcttaat caccgtcttc gagaagacga accatggcgc cagtttcaga atccaagttt
     3721 ctaatatagt tcatcacaat gttgacgttg gtccaacctc cggcttgcat aatctctggg
     3781 atggaaactc cagcgcgagc catgtcacgg gcagctccga cacgggcgga atgtccagac
     3841 caagccaagt aacgttgtcc ggaatcatcc ttggctccgt agatgagacg gtgggtagct
     3901 tcgaagattc ctgaaaattt attttcagct tattcttagc taatatgttg tttaaactta
     3961 ccttcaagag ctcgagtcga gagttgtgat gttgcactcg gggcagcaac tccgttcttg
     4021 cgaacacgac agaacaggta gttgtttgga tcatcggcga ctccggaaac cgaaatccag
     4081 cgctcaacga gcttggtaac tccaagggag agcgcttttt cgactccggc agtcgagacc
     4141 agcgtttttg tacgtccgat gtggatgagc attcttcctc cgtctgtgcg actaatatcc
     4201 ttgacgcgaa tgcgggcaat ttcggcgata cggagaagtg tgttgtaagc aattccgagg
     4261 aaggcgaggt ttcggatgtc ctggcaacga tcggaatttt ccataagaga acggacctga
     4321 tcgaagtcgg ttctgaaaat ttcgtcaagt cttaggattg aatacattaa tatttaaact
     4381 tacctttcga aggcgagggc ttgcttggct ctctctccag catcaacatt ttccttgcgg
     4441 atacgtctca ttacgagaga tacggcatta gaatctgatg ggcgtggaag tccagatcgg
     4501 cgatggagca tattgagctg tccgagatgt tgttggatcg tcttcaccgc caatccacga
     4561 gcttggaggt agagcaagta gtcacgaacg tcttctggct cggctgggaa ccacttgcgg
     4621 ttgttaagct tgcaccaggc agcccaagaa cggcacactg agagaagcat cttccaggtg
     4681 tgctcggaga aggcttgtct gtcacggaac atatccatga ggttcttgcg aacttcatca
     4741 cttgtcgcgt caactggaag tgctggaaga ttttgatgaa cagtgagaag atttgacata
     4801 ttttcgaagt tttttagatg cactagaaca aagcgtgttg gcttcctctg agcccgcttt
     4861 ccttatatac ccgcattctg cagccttaca gaatgttcta gaaggtccta gatgcattcg
     4921 tttgaaaata ctcccggtgg gtgcaaagag acgcagacgg aaaatgtatc tgggtctctt
     4981 tattgtgtac actacttttc catgtaccga atgtgagtcg ccctcctttt gcaacaagca
     5041 gctcgaatgt tctagaaaaa ggtggaaaat agtataaata ccgttgaaaa taaataccga
     5101 acaacatttg ctctaattgt gaaattagaa atcttcaaac tataatcatg tcactttacc
     5161 actatttccg tccagctcaa cgttccgttt ttggtgatca tttttgcttt cgtcgtaaat
     5221 ctacacacgc gtctcttccg tgcgagagtc caagccagca gccaaattcg ttgactgagt
     5281 attcaacgtt tatacgttgt cggcaacgag aaataggaaa atgcatcggg aaatgttctt
     5341 ttttcgattt tttccaaggt tttgacaaat tttaccacga attttgctat gttttcaatt
     5401 aaaaaatatg ttattcaact gtttctatga ggaaaataag gctttgcatg taattttctt
     5461 attcagcata atttttaatt aatttgaatt ttctgtccta acgtttattt tgttttcttg
     5521 gttatgactg atctgaaatt aatttttgaa ttttaaggta atatgtcagg cggtgccgca
     5581 agtttgtaca aaaaagcagg ctccatgaaa aagcctgaac tcaccgcgac gtctgtcgag
     5641 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
     5701 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     5761 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
     5821 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc
     5881 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
     5941 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
     6001 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
     6061 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
     6121 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     6181 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
     6241 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
     6301 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
     6361 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
     6421 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
     6481 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
     6541 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
     6601 cccagcactc gtccgagggc aaaggaatag acccagcttt cttgtacaaa gtgggtccaa
     6661 ttactcttca acatccctac atgctctttc tccctgtgct cccaccccct atttttgtta
     6721 ttatcaaaaa acttctctta atttctttgt tttttagctt cttttaagtc acctctaaca
     6781 atgaaattgt gtagattcaa aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt
     6841 gctccctccc cccattaata ataattctat cccaaaatct acacaatgtt ctgtgtacac
     6901 ttcttatgtt ttttacttct gataaatttt tttgaaacat catagaaaaa accgcacaca
     6961 aaatacctta tcatatgtta cgtttcagtt tatgaccgca atttttaata acttcgtata
     7021 atgtatacta tacgaagtta ttttcagggt ggcggcagcg gaggagagaa tctgtacttt
     7081 caatccggat ccggaggtgg cgggatgcct aaagatccag ccaaacctcc ggccaaggca
     7141 caagttgtgg gatggccacc ggtgagatca taccggaaga acgtgatggt ttcctgccaa
     7201 aaatcaagcg gtggcccgga ggcggcggcg ttcgtgaagg gtggtggcgg ttctggctca
     7261 ggatctgact acaaagacca tgacggtgat tataaagatc atgatatcga ttacaaggat
     7321 gacgatgaca agacgcgaag agcgccatcc agctgatatc ccctatagtg agtcgtatta
     7381 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     7441 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     7501 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     7561 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     7621 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     7681 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     7741 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     7801 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     7861 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     7921 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     7981 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     8041 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     8101 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     8161 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     8221 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     8281 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     8341 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     8401 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     8461 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     8521 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     8581 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     8641 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     8701 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     8761 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     8821 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     8881 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8941 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     9001 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     9061 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacat
//
