LOCUS       sequence-320631-        8816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Dox repressible shPOLE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Sviderskiy VO, Blumenberg L, Gorodetsky E, Karakousi TR, Hirsh N, 
            Alvarez SW, Terzi EM, Kaparos E, Whiten GC, Ssebyala S, Tonzi P, Mir
            H, Neel BG, Huang TT, Adams S, Ruggles KV, Possemato R
  TITLE     Hyperactive CDK2 Activity in Basal-like Breast Cancer Imposes a 
            Genome Integrity Liability that Can Be Exploited by Targeting DNA 
            Polymerase epsilon.
  JOURNAL   Mol Cell. 2020 Oct 28. pii: S1097-2765(20)30723-1. doi: 
            10.1016/j.molcel.2020.10.016.
  PUBMED    33152268
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2020 Oct 28. pii: S1097-2765(20)30723-1. doi: 
            10.1016/j.molcel.2020.10.016."
COMMENT     Sequence Label: Tet-pLKO-puro shPOLE1_1
FEATURES             Location/Qualifiers
     source          1..8816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     84..101
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    218..239
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(254..259,260..277,278..284)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    292..308
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     297..319
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     316..332
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     316..332
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     351..371
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        353..371
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        399..625
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             626..806
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    853..978
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1471..1704
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1889..1933
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2222..2316
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     2233..2252
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    2270..2288
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2298..2316
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    2417..2534
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2583..3093
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3012..3030
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          3102..3674
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     3682..3701
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        3729..3747
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3758..4378
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(3831..3850)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    4413..4986
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4580..4597)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4807..4826
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5006..5605
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5006..5025)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5502..5522
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5733..5966
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6038..6159
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6075..6094)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6129..6148
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6199..6334
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6355..6373)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6383..6400)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6383..6399)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6392..6414)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6541..6996
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6628..6647)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6838..6859
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7022..7126
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7127..7195,7196..7987)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7345..7364)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8158..8746
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8647..8666
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
       61 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
      121 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
      181 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
      241 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
      301 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
      361 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
      421 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
      481 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
      541 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
      601 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
      661 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
      721 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
      781 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
      841 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
      901 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
      961 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     1021 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     1081 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     1141 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     1201 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     1261 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     1321 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     1381 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     1441 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     1501 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     1561 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     1621 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     1681 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     1741 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     1801 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     1861 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     1921 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     1981 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     2041 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     2101 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     2161 cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagcctc gagcggccgc
     2221 aatatttgca tgtcgctatg tgttctggga aatcaccata aacgtgaaat ccctatcagt
     2281 gatagagact tataagttcc ctatcagtga tagagacacc gggcagtgga ttactacttt
     2341 attctcgaga ataaagtagt aatccactgc tttttgaatt ctcgacctcg agacaaatgg
     2401 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     2461 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     2521 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg ccgcggctcg
     2581 agggggttgg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc
     2641 tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg
     2701 tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt
     2761 cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa
     2821 acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca
     2881 gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc
     2941 agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc
     3001 ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc
     3061 ctcgttgacc gaatcaccga cctctctccc cagggggatc tgtgagtttg gggacccttg
     3121 attgttcttt ctttttcgct attgtaaaat tcatgttata tggagggggc aaagttttca
     3181 gggtgttgtt tagaatggga agatgtccct tgtatcacca tggaccctca tgataatttt
     3241 gtttctttca ctttctactc tgttgacaac cattgtctcc tcttattttc ttttcatttt
     3301 ctgtaacttt ttcgttaaac tttagcttgc atttgtaacg aatttttaaa ttcacttttg
     3361 tttatttgtc agattgtaag tactttctct aatcactttt ttttcaaggc aatcagggta
     3421 tattatattg tacttcagca cagttttaga gaacaattgt tataattaaa tgataaggta
     3481 gaatatttct gcatataaat tctggctggc gtggaaatat tcttattggt agaaacaact
     3541 acatcctggt catcatcctg cctttctctt tatggttaca atgatataca ctgtttgaga
     3601 tgaggataaa atactctgag tccaaaccgg gcccctctgc taaccatgtt catgccttct
     3661 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
     3721 aagaattgta atacgactca ctatagggcg aattgatatg tctagattag ataaaagtaa
     3781 agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg
     3841 taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa
     3901 gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg
     3961 ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg
     4021 tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa
     4081 acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga
     4141 gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga
     4201 tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc
     4261 attattacga caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt
     4321 cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc
     4381 gtacagcggc tcccgggagt tctagggatc tgcccctctc cctccccccc ccctaacgtt
     4441 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     4501 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     4561 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     4621 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     4681 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     4741 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     4801 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     4861 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     4921 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     4981 tgataaggat ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
     5041 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     5101 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     5161 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     5221 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     5281 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     5341 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     5401 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     5461 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     5521 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     5581 catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag
     5641 gagcgcacga ccccatgcat cggtaccttt aagaccaatg acttacaagg cagctgtaga
     5701 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     5761 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     5821 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5881 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5941 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     6001 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     6061 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6121 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     6181 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6241 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     6301 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     6361 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     6421 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     6481 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     6541 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6601 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6661 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6721 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6781 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6841 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6901 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6961 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     7021 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7081 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7141 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7201 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7261 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7321 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7381 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7441 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7501 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7561 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7621 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7681 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7741 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7801 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7861 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7921 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7981 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8041 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8101 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8161 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8221 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8281 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8341 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8401 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8461 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8521 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8581 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8641 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8701 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8761 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctg
//
