LOCUS       sequence-320680-       11094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeted integration of SARS-CoV-2 spike HexaPro variant into the 
            AAVS1 genomic safe harbor locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11094)
  AUTHORS   Rivest JF, Goupil C, Doyon Y
  TITLE     Artisanal production of prefusion-stabilized SARS-CoV-2 spikes
  JOURNAL   protocols.io 2021
REFERENCE   2  (bases 1 to 11094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "protocols.io 2021"
COMMENT     Sequence Label: AAVS1_puro_CAG_HexaPro
FEATURES             Location/Qualifiers
     source          1..11094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..23
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(61..83)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     183..202
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    352..1155
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    1162..1187
                     /label=SA
                     /note="splice acceptor site"
     CDS             1211..1264
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1268..1864
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     1761..1781
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    1880..2001
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1891..1910)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1945..1964
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     enhancer        2392..2695
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2697..2972
                     /label=chicken beta-actin promoter
     intron          2973..3989
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3913..3935
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             7797..7820
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7824..7847
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             7854..7877
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7917..7940
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    7979..8203
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    8223..9059
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(9136..9153)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9307..9895)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9387..9406)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10066..10857,10858..10926))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10689..10708
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10927..11031)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
       61 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
      121 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
      181 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
      241 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
      301 ggaagggcga tcggtgcggg cctcttcgct attacgccag aattcgccct ttgctttctc
      361 tgaccagcat tctctcccct gggcctgtgc cgctttctgt ctgcagcttg tggcctgggt
      421 cacctctacg gctggcccag atccttccct gccgcctcct tcaggttccg tcttcctcca
      481 ctccctcttc cccttgctct ctgctgtgtt gctgcccaag gatgctcttt ccggagcact
      541 tccttctcgg cgctgcacca cgtgatgtcc tctgagcgga tcctccccgt gtctgggtcc
      601 tctccgggca tctctcctcc ctcacccaac cccatgccgt cttcactcgc tgggttccct
      661 tttccttctc cttctggggc ctgtgccatc tctcgtttct taggatggcc ttctccgacg
      721 gatgtctccc ttgcgtcccg cctccccttc ttgtaggcct gcatcatcac cgtttttctg
      781 gacaacccca aagtaccccg tctccctggc tttagccacc tctccatcct cttgctttct
      841 ttgcctggac accccgttct cctgtggatt cgggtcacct ctcactcctt tcatttgggc
      901 agctccccta ccccccttac ctctctagtc tgtgctagct cttccagccc cctgtcatgg
      961 catcttccag gggtccgaga gctcagctag tcttcttcct ccaacccggg cccctatgtc
     1021 cacttcagga cagcatgttt gctgcctcca gggatcctgt gtccccgagc tgggaccacc
     1081 ttatattccc agggccggtt aatgtggctc tggttctggg tacttttatc tgtcccctcc
     1141 accccacagt ggggcaagct tctgacctct tctcttcctc ccacagggcc tcgagagatc
     1201 tggcggcgga gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg
     1261 ccctagcacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     1321 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     1381 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     1441 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     1501 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     1561 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     1621 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     1681 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     1741 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     1801 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     1861 ctgatctaga cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1921 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1981 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     2041 ggggaggtgt gggaggtttt ttaatgcatc cacggggttg gggttgcgcc ttttccaagg
     2101 cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg
     2161 cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc
     2221 cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc
     2281 cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagttatta
     2341 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     2401 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     2461 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     2521 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     2581 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     2641 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga
     2701 ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt
     2761 gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggsgcg
     2821 cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg
     2881 cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc
     2941 ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg
     3001 tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc
     3061 cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat
     3121 gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt
     3181 tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt
     3241 gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc
     3301 tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct
     3361 gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg
     3421 gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc
     3481 cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg
     3541 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg
     3601 gggaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc
     3661 attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg
     3721 cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt
     3781 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc
     3841 cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac
     3901 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagcgcc tctgctaacc
     3961 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt gttgtgctgt
     4021 ctcatcattt tggcaaagaa ttgcggccgt ctcaggccga gttcggtacc gccaccatgt
     4081 tcgtgttcct ggtgctcctg cctctggtga gcagccagtg cgtgaacctg accacccgaa
     4141 cccagctccc accagcctac accaacagct ttacacgggg cgtgtactac cctgacaagg
     4201 tgttcagatc tagcgtcctg cacagcactc aggacctctt cctgccgttc ttcagcaacg
     4261 tgacatggtt ccacgccatc cacgtgagcg gcacaaacgg aaccaagcgg tttgataacc
     4321 ccgtcctgcc attcaatgat ggagtttact tcgccagtac cgagaagagt aacatcatcc
     4381 ggggctggat cttcggcacc accctggata gcaaaacaca gagcctcctg atcgtgaaca
     4441 atgccacgaa cgtcgtgatc aaggtgtgcg agttccagtt ttgcaatgat cctttcctgg
     4501 gtgtgtacta ccacaagaac aacaagagct ggatggaaag cgagttcaga gtctacagca
     4561 gcgccaacaa ctgcacattc gagtacgtct ctcagccttt tctgatggac cttgagggga
     4621 aacaaggcaa cttcaagaac ctgagagaat tcgtgttcaa gaacatcgac ggctacttca
     4681 aaatctactc caagcacaca cccatcaacc tggtccggga cctccctcag ggcttcagcg
     4741 ccctggaacc cctggtcgac ctgcccatag gcatcaacat aacgcggttc caaaccctgc
     4801 tggccctgca tagatcctac ctgactcctg gcgacagcag cagcggatgg accgccggag
     4861 ctgcagccta ctatgtgggc tacctgcaac ctagaacctt cctgctgaag tacaacgaga
     4921 acggcacaat cacagacgcc gtcgactgcg ccctggaccc tctctctgag acaaagtgca
     4981 ccctgaagtc cttcaccgtg gaaaagggca tctaccagac cagcaacttc cgggtgcagc
     5041 ctacagagag catcgtgcga tttccaaaca ttaccaacct ctgccccttc ggcgaggtgt
     5101 ttaacgccac aagatttgcc tccgtttacg cctggaatag aaagagaatc agcaattgtg
     5161 tggccgacta ctccgtgctg tataacagcg cctctttcag caccttcaag tgctacggcg
     5221 tttccccaac aaagctgaat gacctgtgct tcaccaacgt gtacgccgac tccttcgtaa
     5281 ttagaggcga tgaggtgcgg cagatcgcac caggccagac cggtaagatc gctgactaca
     5341 actataagct gcctgatgat tttacaggct gcgtgatcgc ctggaactct aacaacctgg
     5401 atagcaaggt gggcggcaac tacaactacc tgtaccggct gtttcgcaag tctaacctga
     5461 aacctttcga gagagacatc tccacagaga tctaccaggc cggttctaca ccttgtaacg
     5521 gggtggaagg cttcaactgt tacttccctc tgcaaagcta cggcttccag cctaccaatg
     5581 gagtcggcta ccagccatac cgggtggtcg tgctgtcctt cgagttactc cacgcccccg
     5641 ccaccgtctg cggtcctaag aagtccacca atctggttaa gaacaaatgc gtgaacttca
     5701 acttcaacgg cctgaccggg accggcgtgc tgaccgaaag caacaaaaag ttcctcccct
     5761 tccagcagtt cggccgtgat atcgctgaca ccacagatgc cgtcagagat ccacagaccc
     5821 tggaaatcct ggatattaca ccctgctcct tcggaggagt ttctgtgatc acccccggga
     5881 ccaataccag caaccaggtg gctgtgctgt accaagatgt taactgcacc gaggttcctg
     5941 tggccatcca cgccgatcag ctgacaccta cttggagagt gtactccact ggctccaatg
     6001 tgttccagac cagggccgga tgtctgatcg gcgccgagca cgtgaataac agttacgagt
     6061 gcgacatccc tatcggcgcc ggcatctgtg ccagctacca gacccagaca aacagccctg
     6121 ggtctgcttc ctctgtagct agccagagca tcatcgccta caccatgagc ctgggcgcag
     6181 agaacagcgt ggcctattcc aacaactcta tcgccattcc caccaacttt acaattagcg
     6241 tcacaacaga gatcctgccc gtgagcatga ccaagaccag cgtggactgt acaatgtaca
     6301 tctgtggcga cagcactgaa tgcagcaacc tgctgctgca atacggctcc ttttgcaccc
     6361 aactgaaccg ggcgctgacc ggaatcgccg tggaacagga caaaaatacc caggaggtgt
     6421 tcgcccaagt gaagcagatc tacaagaccc cacctatcaa ggacttcggc ggctttaact
     6481 ttagccagat tctccctgat ccttctaagc ctagcaagcg gagccctatc gaggatctgc
     6541 tgttcaacaa ggtcaccctg gccgatgccg gctttatcaa acagtatggc gattgcctgg
     6601 gcgacatagc cgccagagat ctgatctgcg cccagaaatt caacggcctg acagttctcc
     6661 cacctctgct gaccgacgag atgatcgctc agtacacctc tgccctgctg gctggcacca
     6721 tcacatctgg gtggacattt ggcgccggcc ctgccctgca gatccccttt cccatgcaga
     6781 tggcctatag attcaacgga atcggcgtga cccagaacgt gctgtatgaa aaccagaagc
     6841 tgatcgctaa ccagttcaat tctgccatcg gcaagatcca ggactccctc tcctctaccc
     6901 ctagcgccct gggcaaactg caggacgtgg tgaatcagaa cgcccaagcc ctgaacaccc
     6961 tggtgaagca gctcagcagc aattttggcg ccatcagctc tgtgctgaac gatatcctgt
     7021 ctagactgga ccctccagaa gccgaagtcc agatcgatag actgatcaca ggcagactgc
     7081 agtccctgca aacctacgtg acccaacagc tgatcagggc cgctgaaata agagccagcg
     7141 ccaatctcgc cgctaccaag atgtccgagt gtgtgctggg acagtctaaa cgcgttgact
     7201 tctgcggcaa aggctatcac ctgatgagct tcccccagag cgcgccgcac ggcgtggtgt
     7261 tcctgcatgt gacatacgtg cctgcccaag agaagaattt cacaaccgcc cctgccatct
     7321 gccacgacgg caaggcccac ttcccaagag agggcgtttt cgtttccaat ggcacacact
     7381 ggttcgtgac acaaagaaac ttctacgaac cccagattat caccaccgac aacaccttcg
     7441 tgagtggcaa ttgtgacgtg gtcatcggaa tcgtgaacaa cacagtgtac gaccctctgc
     7501 aacctgagct ggactctttt aaggaagagc tggacaagta ctttaaaaac cacaccagcc
     7561 ccgatgtgga cctgggcgac atcagtggca ttaacgccag cgtggtgaac atccaaaagg
     7621 aaatcgacag actgaacgag gtggccaaga acctgaacga gtccctgatc gacctgcagg
     7681 agctcggcaa atacgagcag ggatccggat acatccccga ggcccccaga gatggccagg
     7741 cctacgtgcg gaaggacggc gagtgggtac tgctgagcac attcctgggc agatccctgg
     7801 aggtgctgtt ccagggccca ggccatcacc accatcacca ccatcatagc gcctggtccc
     7861 acccccagtt cgagaagggc ggcggtagtg gagggggcgg atctggcggc tcagcttgga
     7921 gccaccccca gttcgaaaag tgataatgac tcgagcggcc gcgtttaaac cctgcaggct
     7981 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     8041 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     8101 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     8161 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tgggtcgaca gtactaagct
     8221 ttactaggga caggattggt gacagaaaag ccccatcctt aggcctcctc cttcctagtc
     8281 tcctgatatt gggtctaacc cccacctcct gttaggcaga ttccttatct ggtgacacac
     8341 ccccatttcc tggagccatc tctctccttg ccagaacctc taaggtttgc ttacgatgga
     8401 gccagagagg atcctgggag ggagagcttg gcagggggtg ggagggaagg gggggatgcg
     8461 tgacctgccc ggttctcagt ggccaccctg cgctaccctc tcccagaacc tgagctgctc
     8521 tgacgcggct gtctggtgcg tttcactgat cctggtgctg cagcttcctt acacttccca
     8581 agaggagaag cagtttggaa aaacaaaatc agaataagtt ggtcctgagt tctaactttg
     8641 gctcttcacc tttctagtcc ccaatttata ttgttcctcc gtgcgtcagt tttacctgtg
     8701 agataaggcc agtagccagc cccgtcctgg cagggctgtg gtgaggaggg gggtgtccgt
     8761 gtggaaaact ccctttgtga gaatggtgcg tcctaggtgt tcaccaggtc gtggccgcct
     8821 ctactccctt tctctttctc catccttctt tccttaaaga gtccccagtg ctatctggga
     8881 catattcctc cgcccagagc agggtcccgc ttccctaagg ccctgctctg ggcttctggg
     8941 tttgagtcct tggcaagccc aggagaggcg ctcaggcttc cctgtccccc ttcctcgtcc
     9001 accatctcat gcccctggct ctcctgcccc ttccctacag gggttcctgg ctctgctcta
     9061 agggcgaatt ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     9121 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     9181 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     9241 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     9301 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     9361 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9421 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9481 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9541 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9601 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9661 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9721 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     9781 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9841 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9901 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9961 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    10021 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    10081 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    10141 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    10201 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    10261 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    10321 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    10381 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    10441 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    10501 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10561 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10621 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10681 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10741 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10801 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10861 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10921 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10981 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    11041 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa ccta
//
