LOCUS       sequence-320720-        3747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  protein expression in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Shen H, Forgacs D, Chapla D, Moremen KW, Wells L, Hamer SA, Tompkins
            SM, Ross TM, Rouphael N, Edupuganti S, Collins MH, Tarleton RL
  TITLE     A flexible, pan-species, multi-antigen platform for the detection 
            and monitoring of SARS-CoV-2-specific antibody responses.
  JOURNAL   medRxiv [Preprint]. 2021 Jan 27:2021.01.20.21249279. doi: 
            10.1101/2021.01.20.21249279.
  PUBMED    33532799
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2021 Jan 27:2021.01.20.21249279. doi: 
            10.1101/2021.01.20.21249279."
COMMENT     Sequence Label: pET30a-Spike-E
FEATURES             Location/Qualifiers
     source          1..3747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3721..3744
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 atgttcgttt tcctggttct gctgccgctg gttagcagcc aatgcgtgaa tctgaccacc
       61 cgcacccaac tgccgccggc gtacaccaac agcttcaccc gtggtgttta ctatccggac
      121 aaagtttttc gtagcagcgt gctgcacagc acccaggacc tgttcctgcc gttctttagc
      181 aacgttacct ggttccacgc gatccacgtg agcggcacca acggcaccaa gcgtttcgac
      241 aacccggtgc tgccgtttaa cgatggtgtt tacttcgcga gcaccgagaa gagcaacatc
      301 attcgtggtt ggatttttgg caccaccctg gacagcaaaa cccagagcct gctgatcgtt
      361 aacaacgcga ccaacgtggt tattaaggtg tgcgaattcc aattctgcaa cgatccgttc
      421 ctgggcgttt actatcacaa gaacaacaaa agctggatgg agagcgaatt tcgtgtttat
      481 agcagcgcga acaactgcac ctttgagtac gtgagccagc cgttcctgat ggacctggaa
      541 ggcaagcaag gcaacttcaa aaacctgcgt gagttcgtgt tcaagaacat cgatggttac
      601 ttcaagatct acagcaagca caccccgatc aacctggttc gtgacctgcc gcagggtttt
      661 agcgcgctgg agccgctggt tgacctgccg atcggcatta acatcacccg ttttcaaacc
      721 ctgctggcgc tgcaccgtag ctacctgacc ccgggtgaca gcagcagcgg ttggaccgcg
      781 ggtgcggcgg cgtactatgt tggttatctg cagccgcgta ccttcctgct gaaatacaac
      841 gaaaacggca ccatcaccga cgcggttgat tgcgcgctgg atccgctgag cgaaaccaag
      901 tgcaccctga aaagctttac cgtggagaag ggtatttatc agaccagcaa cttccgtgtg
      961 caaccgaccg aaagcattgt tcgttttccg aacatcacca acctgtgccc gtttggcgag
     1021 gttttcaacg cgacccgttt cgcgagcgtg tacgcgtgga accgtaaacg tatcagcaac
     1081 tgcgttgcgg actatagcgt gctgtacaac agcgcgagct tcagcacctt taagtgctat
     1141 ggtgtgagcc cgaccaaact gaacgatctg tgctttacca acgtttacgc ggatagcttc
     1201 gtgattcgtg gcgacgaggt tcgtcagatc gcgccgggtc aaaccggcaa gattgcggac
     1261 tacaactata aactgccgga cgatttcacc ggttgcgtta tcgcgtggaa cagcaacaac
     1321 ctggatagca aagtgggtgg caactacaac tatctgtacc gtctgtttcg taagagcaac
     1381 ctgaaaccgt tcgagcgtga cattagcacc gaaatctacc aggcgggtag caccccgtgc
     1441 aacggtgttg agggctttaa ctgctatttc ccgctgcaga gctacggctt ccaaccgacc
     1501 aacggtgtgg gctatcaacc gtaccgtgtg gttgtgctga gctttgaact gctgcatgcg
     1561 ccggcgaccg tttgcggtcc gaagaaaagc accaacctgg ttaagaacaa atgcgtgaac
     1621 ttcaacttta acggcctgac cggcaccggt gtgctgaccg agagcaacaa gaaattcctg
     1681 ccgtttcagc aattcggtcg tgacattgcg gataccaccg atgcggtgcg tgacccgcag
     1741 accctggaaa ttctggatat caccccgtgc agcttcggtg gcgttagcgt gatcaccccg
     1801 ggcaccaaca ccagcaacca ggttgcggtg ctgtatcaag acgttaactg caccgaggtt
     1861 ccggtggcga ttcacgcgga tcagctgacc ccgacctggc gtgtgtacag caccggtagc
     1921 aacgttttcc aaacccgtgc gggttgcctg attggtgcgg agcatgtgaa caacagctat
     1981 gaatgcgaca ttccgatcgg tgcgggcatt tgcgcgagct accagaccca aaccaacagc
     2041 ccgggtagcg cgagcagcgt tgcgagccag agcatcattg cgtataccat gagcctgggc
     2101 gcggaaaaca gcgtggcgta cagcaacaac agcattgcga tcccgaccaa cttcaccatt
     2161 agcgtgacca ccgagatcct gccggttagc atgaccaaaa ccagcgtgga ctgcaccatg
     2221 tatatctgcg gtgatagcac cgaatgcagc aacctgctgc tgcagtacgg tagcttttgc
     2281 acccaactga accgtgcgct gaccggcatt gcggtggagc aggataaaaa cacccaagaa
     2341 gttttcgcgc aggtgaagca aatttacaaa accccgccga tcaaggactt tggtggcttc
     2401 aactttagcc agatcctgcc ggatccgagc aagccgagca aacgtagctt tattgaggac
     2461 ctgctgttca acaaggttac cctggcggat gcgggtttca tcaaacagta tggtgattgc
     2521 ctgggcgaca ttgcggcgcg tgacctgatc tgcgcgcaaa agtttaacgg cctgaccgtg
     2581 ctgccgccgc tgctgaccga tgaaatgatt gcgcagtaca ccagcgcgct gctggcgggc
     2641 accattacca gcggttggac ctttggtgcg ggtgcggcgc tgcagatccc gtttgcgatg
     2701 caaatggcgt atcgtttcaa cggtattggc gttacccaaa acgtgctgta cgagaaccag
     2761 aagctgatcg cgaaccaatt taacagcgcg attggtaaaa tccaggatag cctgagcagc
     2821 accgcgagcg cgctgggtaa actgcaagat gttgtgaacc agaacgcgca agcgctgaac
     2881 accctggtta agcagctgag cagcaacttc ggtgcgatta gcagcgtgct gaacgatatt
     2941 ctgagccgtc tggacccgcc ggaggcggaa gttcaaattg accgtctgat caccggccgt
     3001 ctgcagagcc tgcaaaccta tgtgacccag caactgattc gtgcggcgga aattcgtgcg
     3061 agcgcgaacc tggcggcgac caaaatgagc gagtgcgttc tgggtcagag caagcgtgtg
     3121 gacttttgcg gtaaaggcta tcacctgatg agcttcccgc agagcgcgcc gcacggtgtt
     3181 gtgtttctgc acgttaccta cgtgccggcg caagaaaaga actttaccac cgcgccggcg
     3241 atctgccatg atggtaaagc gcactttccg cgtgaaggtg ttttcgtgag caacggcacc
     3301 cactggtttg ttacccagcg taacttctac gagccgcaaa tcattaccac cgacaacacc
     3361 ttcgtgagcg gtaactgcga tgttgtgatt ggcatcgtta acaacaccgt gtatgatccg
     3421 ctgcagccgg agctggacag ctttaaagag gagctggata agtacttcaa aaaccacacc
     3481 agcccggacg ttgatctggg tgacattagc ggcatcaacg cgagcgttgt gaacattcaa
     3541 aaggagatcg accgtctgaa cgaagtggcg aaaaacctga acgaaagcct gatcgatctg
     3601 caggagctgg gtaaatatga acaaggtagc ggctacattc cggaggcgcc gcgtgacggt
     3661 caggcgtatg tgcgtaagga tggcgagtgg gttctgctga gcacctttct gggtcgtagc
     3721 ctggaagttc tgtttcaagg tccgggt
//
