LOCUS       sequence-320763-        9117 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR repair template: add insertion site specific homology arms by
            PCR before insertion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9117)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 9117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pJW1927
FEATURES             Location/Qualifiers
     source          1..9117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1445..1478
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             1488..1517
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3334..3657)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5575..5599
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5602..6627
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5602..6627
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6628..6652)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    7005..7038
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             7063..7083
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             7105..7236
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             7264..7329
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(7358..7377)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7359..7377)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7382..7398)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7382..7398)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7500..7519)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7511..8320
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7578..7597)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      8467..9055
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8956..8975
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgctc ttcgatggtc tccaaaggag
      601 aagctgtgat caaggagttc atgcgcttca aggttcacat ggaaggaagc atgaatggtc
      661 acgagttcga aatcgaagga gaaggggagg gccgcccgta cgagggaact cagaccgcca
      721 agctgaaggt caccaaggga ggtaagtttg tgataatcca atttcaattc gcaatggtca
      781 tcgttttttc aggaccactt ccattctcat gggatattct ctccccacaa ttcatgtacg
      841 gctcccgtgc tttcatcaaa cacccagccg acattccaga ttactacaag caatctttcc
      901 cagaaggctt caagtgggag cgtgtgatga acttcgagga tggtggagca gttacagtaa
      961 ctcaggacac ttctctcgag gtaagttttt actccgcttt taacaatggt tgtttgacat
     1021 cattttttca ggatggcact ttgatctaca aggtcaagct ccgtggtacc aatttcccac
     1081 cagatggacc agttatgcag aagaagacga tgggatggga ggcttccacc gaacgattgt
     1141 acccagaaga tggagttctc aagggagaca tcaaaatggc gttacgcctc aaggacggag
     1201 gtaagtttga tgaaacggtt tcgtcttata tacactaatg gtacttttca ggacgttacc
     1261 tggcggattt caagaccaca tacaaggcaa agaagccagt tcaaatgcca ggagcatata
     1321 acgttgaccg caagcttgat attacttccc ataatgaaga ctacacagtt gtagaacaat
     1381 acgaacgatc cgagggacgt cattcgaccg gaggaatgga cgagctgtac aaggtaagtt
     1441 taaaataact tcgtataatg tatactatac gaagttattt tcagggagag caaaagctta
     1501 tctccgagga ggacctctaa ggatgatcga cgccaacgtc gttgaatttt caaattttaa
     1561 atactgaata tttgtttttt ttcctattat ttatttattc tctttgtgtt ttttttcttg
     1621 ctttctaaaa aattaattca atccaaatct aaacattttt ttttctcttt ccgtctccca
     1681 attcgtattc cgctcctctc atctgaacac aatgtgcaag tttatttatc ttctcgcttt
     1741 catttcatta ggacgtgggg ggaattggtg gaagggggaa acacacaaaa ggatgatgga
     1801 aatgaaataa ggacacacaa tatgcaacaa cattcaattc agaaatatgg aggaaggttt
     1861 aaaagaaaac ataaaaatat atagaggagg aaggaaacta gctgccctcg attgtctatc
     1921 ttccattctc accaagtttc aacaacttta ttcagtcatt cttctctggt tcatttccca
     1981 accaatcttc ccgaataaag cccatctgtc ctttcggcca cattcctcac ctacatccgt
     2041 actccttatc tcccgttctt atccagtctt ttatcgtctc tgcgtctcgc acactgtctc
     2101 gtcggtaatg taaaaaaaaa acaaaaaaaa cttgaaaact tgcggaaaga tttcctaata
     2161 aaaggagacg aatccggtag gaaatcgaca agtctctgct tctcgaagac taggtgagtt
     2221 acatttcttt ggccgaatta ttttgaaatc ctaaatttga taatttcaga gatgtctgta
     2281 aaacttgcgt gttatgtgac ggcttcggtc actgtcgcca ctcttatggt atgcttcatg
     2341 accatgtcaa ccatctactc ggaggttgat ggattcagag agaagcttga taccgagatg
     2401 aatgtgttca gagtgagtta aattatattt tgaattttaa ataattttta attttctagc
     2461 aatctaccaa tggattgtgg aaggacatag ttgtcatcgg aagatctagc aagcgtgtcc
     2521 gttgtcaata tgaagagacc aacgctaccc caactccaca tgctgatgga tccccatctg
     2581 ctccaccagg tcaaccacca gcagttccac cagtcttcaa ccagccaaag actccaaatg
     2641 gagccaatgg aaatggacca acctgcaact gcaatgctga taacaagtgc ccagctggac
     2701 catccggacc aaagggagtt ccaggagttc caggactcga cggagttcca ggacttgacg
     2761 gtgttccagg agttggagct gatgatatcg ctccacaacg cgagtctgtc ggatgcttca
     2821 cttgcccaca aggaccagtt ggaccaccag gagctcttgg aagaccagga ccacgtggac
     2881 ttccaggacc aagaggacaa aatggaaacc caggaagaga tggacaacca ggacatccag
     2941 gagagcaagg atcatccggt caaatcggaa agatcggaga gccaggacca ccaggagaga
     3001 agggacgcga cgccgagcat ccaatcggaa gaccaggacc aaagggacca agaggagatc
     3061 aaggaccaac aggaccagct ggacagaacg gtcttcacgg accaccagga gagccaggaa
     3121 ccgttggacc agaaggacca tctggaaagc aaggacgtca aggaccagac ggaacccagg
     3181 gagagactgg accagacgga agaccaggaa aggatgccga gtactgccag tgcccagaca
     3241 agtctccacc atcagaggct gtcaacgcca accgtggata cagaaatatc taaattgttg
     3301 gtgttttcta ataaaatatt tgagattcag tgagactttt ttcttggcgg cacaataaag
     3361 tttcctcttt ttgttgggtc actcaacgaa cacaatacaa atatttgagc gctcgcattt
     3421 ttctcaacct ttcgggttta aagcacttca aaatggaatt gaagagagat tgtgaaagta
     3481 catcagacag aatcacattt tgatagcatt cacttcactc agatgcaaac ttttcagtga
     3541 ctataaaaag tcataaaata ataacattaa aaaagcaaat aaattagcga gagaattttt
     3601 tgacaaaaag aaagaagagt gatagagaag aagggaatgc ttgaaaggat tttgcattta
     3661 tcttaatcac cgtcttcgag aagacgaacc atggcgccag tttcagaatc caagtttcta
     3721 atatagttca tcacaatgtt gacgttggtc caacctccgg cttgcataat ctctgggatg
     3781 gaaactccag cgcgagccat gtcacgggca gctccgacac gggcggaatg tccagaccaa
     3841 gccaagtaac gttgtccgga atcatccttg gctccgtaga tgagacggtg ggtagcttcg
     3901 aagattcctg aaaatttatt ttcagcttat tcttagctaa tatgttgttt aaacttacct
     3961 tcaagagctc gagtcgagag ttgtgatgtt gcactcgggg cagcaactcc gttcttgcga
     4021 acacgacaga acaggtagtt gtttggatca tcggcgactc cggaaaccga aatccagcgc
     4081 tcaacgagct tggtaactcc aagggagagc gctttttcga ctccggcagt cgagaccagc
     4141 gtttttgtac gtccgatgtg gatgagcatt cttcctccgt ctgtgcgact aatatccttg
     4201 acgcgaatgc gggcaatttc ggcgatacgg agaagtgtgt tgtaagcaat tccgaggaag
     4261 gcgaggtttc ggatgtcctg gcaacgatcg gaattttcca taagagaacg gacctgatcg
     4321 aagtcggttc tgaaaatttc gtcaagtctt aggattgaat acattaatat ttaaacttac
     4381 ctttcgaagg cgagggcttg cttggctctc tctccagcat caacattttc cttgcggata
     4441 cgtctcatta cgagagatac ggcattagaa tctgatgggc gtggaagtcc agatcggcga
     4501 tggagcatat tgagctgtcc gagatgttgt tggatcgtct tcaccgccaa tccacgagct
     4561 tggaggtaga gcaagtagtc acgaacgtct tctggctcgg ctgggaacca cttgcggttg
     4621 ttaagcttgc accaggcagc ccaagaacgg cacactgaga gaagcatctt ccaggtgtgc
     4681 tcggagaagg cttgtctgtc acggaacata tccatgaggt tcttgcgaac ttcatcactt
     4741 gtcgcgtcaa ctggaagtgc tggaagattt tgatgaacag tgagaagatt tgacatattt
     4801 tcgaagtttt ttagatgcac tagaacaaag cgtgttggct tcctctgagc ccgctttcct
     4861 tatatacccg cattctgcag ccttacagaa tgttctagaa ggtcctagat gcattcgttt
     4921 gaaaatactc ccggtgggtg caaagagacg cagacggaaa atgtatctgg gtctctttat
     4981 tgtgtacact acttttccat gtaccgaatg tgagtcgccc tccttttgca acaagcagct
     5041 cgaatgttct agaaaaaggt ggaaaatagt ataaataccg ttgaaaataa ataccgaaca
     5101 acatttgctc taattgtgaa attagaaatc ttcaaactat aatcatgtca ctttaccact
     5161 atttccgtcc agctcaacgt tccgtttttg gtgatcattt ttgctttcgt cgtaaatcta
     5221 cacacgcgtc tcttccgtgc gagagtccaa gccagcagcc aaattcgttg actgagtatt
     5281 caacgtttat acgttgtcgg caacgagaaa taggaaaatg catcgggaaa tgttcttttt
     5341 tcgatttttt ccaaggtttt gacaaatttt accacgaatt ttgctatgtt ttcaattaaa
     5401 aaatatgtta ttcaactgtt tctatgagga aaataaggct ttgcatgtaa ttttcttatt
     5461 cagcataatt tttaattaat ttgaattttc tgtcctaacg tttattttgt tttcttggtt
     5521 atgactgatc tgaaattaat ttttgaattt taaggtaata tgtcaggcgg tgccgcaagt
     5581 ttgtacaaaa aagcaggctc catgaaaaag cctgaactca ccgcgacgtc tgtcgagaag
     5641 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
     5701 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
     5761 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
     5821 attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc
     5881 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag
     5941 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc
     6001 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
     6061 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
     6121 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
     6181 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
     6241 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
     6301 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
     6361 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa
     6421 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
     6481 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
     6541 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
     6601 agcactcgtc cgagggcaaa ggaatagacc cagctttctt gtacaaagtg ggtccaatta
     6661 ctcttcaaca tccctacatg ctctttctcc ctgtgctccc accccctatt tttgttatta
     6721 tcaaaaaact tctcttaatt tctttgtttt ttagcttctt ttaagtcacc tctaacaatg
     6781 aaattgtgta gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct
     6841 ccctcccccc attaataata attctatccc aaaatctaca caatgttctg tgtacacttc
     6901 ttatgttttt tacttctgat aaattttttt gaaacatcat agaaaaaacc gcacacaaaa
     6961 taccttatca tatgttacgt ttcagtttat gaccgcaatt tttaataact tcgtataatg
     7021 tatactatac gaagttattt tcagggtggc ggcagcggag gagagaatct gtactttcaa
     7081 tccggatccg gaggtggcgg gatgcctaaa gatccagcca aacctccggc caaggcacaa
     7141 gttgtgggat ggccaccggt gagatcatac cggaagaacg tgatggtttc ctgccaaaaa
     7201 tcaagcggtg gcccggaggc ggcggcgttc gtgaagggtg gtggcggttc tggctcagga
     7261 tctgactaca aagaccatga cggtgattat aaagatcatg atatcgatta caaggatgac
     7321 gatgacaaga cgcgaagagc gccatccagc tgatatcccc tatagtgagt cgtattacat
     7381 ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc
     7441 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
     7501 aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat
     7561 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
     7621 aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
     7681 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
     7741 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
     7801 tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
     7861 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg
     7921 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg
     7981 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
     8041 gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt
     8101 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
     8161 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
     8221 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
     8281 taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt
     8341 gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc
     8401 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8461 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8521 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8581 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     8641 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8701 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8761 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8821 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8881 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8941 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     9001 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     9061 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtt
//
