LOCUS       sequence-320766-       11896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant mitoTALEN vector assembly, Destination vector for plants to 
            express ORFs from entry clone with mitochondrial presequence under 
            the RPS5A promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11896)
  AUTHORS   Arimura SI, Ayabe H, Sugaya H, Okuno M, Tamura Y, Tsuruta Y, Watari 
            Y, Yanase S, Yamauchi T, Itoh T, Toyoda A, Takanashi H, Tsutsumi N
  TITLE     Targeted gene disruption of ATP synthases 6-1 and 6-2 in the 
            mitochondrial genome of Arabidopsis thaliana by mitoTALENs.
  JOURNAL   Plant J. 2020 Oct 24. doi: 10.1111/tpj.15041.
  PUBMED    33098708
REFERENCE   2  (bases 1 to 11896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2020 Oct 24. doi: 10.1111/tpj.15041."
COMMENT     Sequence Label: pDESTpK7WG-RPS5Ap-mito
FEATURES             Location/Qualifiers
     source          1..11896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        124..303
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             337..1131
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(391..410)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1001..1020
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1171..1423
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1516..1540
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2061..2852
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3098..3686
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3587..3606
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3840..3857
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3872..4012
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3938..3957)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4098..4120
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4356..4550
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4616..5689)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6118..6747)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8078..8102
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    9944..10068
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(10093..10098,10099..10116,10117..10123)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             10177..10836
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(10243..10262)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             11178..11483
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     11362..11381
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(11524..11648)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gtcgggccct ctagaggatc cccgggtacc gcgaattatc gatcatgagc ggagaattaa
       61 gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg
      121 acagaaccgc aacgttgaag gagccactga gccgcgggtt tctggagttt aatgagctaa
      181 gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta
      241 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
      301 ttagagtctc atattcactc tcaactcgat cgaggcatga ttgaacaaga tggattgcac
      361 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
      421 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
      481 gtcaagaccg acctgtccgg tgccctgaat gaactccaag acgaggcagc gcggctatcg
      541 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
      601 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
      661 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
      721 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
      781 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
      841 gaactgttcg ccaggctcaa ggcgcggatg cccgacggcg aggatctcgt cgtgacccac
      901 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
      961 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     1021 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     1081 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     1141 tggggttcgg actctagcta gagtcaagca gatcgttcaa acatttggca ataaagtttc
     1201 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     1261 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     1321 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     1381 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgaccggc atgcaagctg
     1441 ataattcaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg
     1501 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc
     1561 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg
     1621 cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg
     1681 aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt
     1741 tgattgtaac gatgacagag cgttgctgcc tgtgatcaat tcgggcacga acccagtgga
     1801 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     1861 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     1921 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     1981 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     2041 ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat
     2101 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     2161 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     2221 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     2281 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     2341 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     2401 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     2461 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     2521 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     2581 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     2641 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     2701 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     2761 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     2821 aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga
     2881 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac
     2941 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac
     3001 acaaattggg agatatatca tgcatgacca aaatccctta acgtgagttt tcgttccact
     3061 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3121 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3181 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3241 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3301 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3361 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3421 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3481 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3541 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3601 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3661 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3721 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3781 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3841 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     3901 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     3961 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
     4021 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     4081 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     4141 acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt
     4201 gtccgcgccc tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg
     4261 cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt
     4321 tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa
     4381 gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat
     4441 cagtcactta catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt
     4501 tccggttccc aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac
     4561 cttttcgacc tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg
     4621 aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag
     4681 cctgccccgc ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc
     4741 gcacggtgaa acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct
     4801 tgaacaacca tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac
     4861 ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct
     4921 tgccttctgt gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc
     4981 gcccggtttc gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg
     5041 tcaccaggcg gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta
     5101 accgaatgca ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga
     5161 acttgagtac gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt
     5221 cccggtatcg gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat
     5281 cccacacact ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct
     5341 cgtagcggat cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt
     5401 ccatgatgct gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg
     5461 gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc
     5521 gggattcttt gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg
     5581 cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac
     5641 ccagcgccgc gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc
     5701 gggcttgggg gttccagtgc cattgcaggg ccggcagaca acccagccgc ttacgcctgg
     5761 ccaaccgccc gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg
     5821 attttccatg ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca
     5881 tttactctgg tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc
     5941 gtaccgcgta catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct
     6001 tcatggctgg cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg
     6061 gacggccggc acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca
     6121 aatgagtttt gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc
     6181 gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg
     6241 cgtgatacgg gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc
     6301 gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc
     6361 cgtgacctca atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta
     6421 agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa
     6481 gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac
     6541 gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac
     6601 ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc
     6661 ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt
     6721 ctggttaagt acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat
     6781 cgcatcatat acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt
     6841 tttagacggc ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg
     6901 gcatcagaca aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc
     6961 tcgaacacgt acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt
     7021 tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg
     7081 ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct
     7141 cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc
     7201 caagcagtgc agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt
     7261 gcgcgatctg tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg
     7321 cggcctcgcg cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc
     7381 cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg
     7441 ccaggctacg caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc
     7501 gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt
     7561 ggtcaagcat cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa
     7621 acagcttggt gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt
     7681 cggtgctgac gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat
     7741 gtctccggtt ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac
     7801 gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt
     7861 tttcagaaga cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt
     7921 ggatcaaagt actttaaagt actttaaagt actttaaagt actttgatcc cgaggggaac
     7981 cctgtggttg gcatgcacat acaaatggac gaacggataa accttttcac gcccttttaa
     8041 atatccgatt attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt
     8101 caaacactga tagtttaaac tgaaggcggg aaacgacaat ctgatccaag ctcaagctaa
     8161 gctctcaact tttgattcgc tatttgcagt gcacctgtgg cgttcatcac atcttttgtg
     8221 acactgtttg cactggtcat tgctattaca aaggaccttc ctgatgttga aggagatcga
     8281 aagtaagtaa ctgcacgcat aaccattttc tttccgctct ttggctcaat ccatttgaca
     8341 gtcaaagaca atgtttaacc agctccgttt gatatattgt ctttatgtgt ttgttcaagc
     8401 atgtttagtt aatcatgcct ttgattgatc ttgaataggt tccaaatatc aaccctggca
     8461 acaaaacttg gagtgagaaa cattgcattc ctcggttctg gacttctgct agtaaattat
     8521 gtttcagcca tatcactagc tttctacatg cctcaggtga attcatctat ttccgtctta
     8581 actatttcgg ttaattaaag cacgaacacc attactgcat gtagaagctt gataaactat
     8641 cgccaccaat ttatttttgt tgcgatattg ttactttcct cagtatgcag ctttgaaaag
     8701 accaaccctc ttatccttta acaatgaaca ggtttttaga ggtagcttga tgattcctgc
     8761 acatgtgatc ttggcttcag gcttaatttt ccaggtaaag cattatgaga tactcttata
     8821 tctcttacat acttttgaga taatgcacaa gaacttcata actatatgct ttagtttctg
     8881 catttgacac tgccaaattc attaatctct aatatctttg ttgttgatct ttggtagaca
     8941 tgggtactag aaaaagcaaa ctacaccaag gtaaaatact tttgtacaaa cataaactcg
     9001 ttatcacgga acatcaatgg agtgtatatc taacggagtg tagaaacatt tgattattgc
     9061 aggaagctat ctcaggatat tatcggttta tatggaatct cttctacgca gagtatctgt
     9121 tattcccctt cctctagctt tcaatttcat ggtgaggata tgcagttttc tttgtatatc
     9181 attcttcttc ttctttgtag cttggagtca aaatcggttc cttcatgtac atacatcaag
     9241 gatatgtcct tctgaatttt tatatcttgc aataaaaatg cttgtaccaa ttgaaacacc
     9301 agctttttga gttctatgat cactgacttg gttctaacca aaaaaaaaaa aatgtttaat
     9361 ttacatatct aaaagtaggt ttagggaaac ctaaacagta aaatatttgt atattattcg
     9421 aatttcactc atcataaaaa cttaaattgc accataaaat tttgttttac tattaatgat
     9481 gtaatttgtg taacttaaga taaaaataat attccgtaag ttaaccggct aaaaccacgt
     9541 ataaaccagg gaacctgtta aaccggttct ttactggata aagaaatgaa agcccatgta
     9601 gacagctcca ttagagccca aaccctaaat ttctcatcta tataaaagga gtgacattag
     9661 ggtttttgtt cgtcctctta aagcttctcg ttttctctgc cgtctctctc attcgcgcga
     9721 cgcaaacgat cttcaggtga tcttctttct ccaaatcctc tctcataact ctgatttcgt
     9781 acttgtgtat ttgagctcac gctctgtttc tctcaccaca gccatgttta aacaagcttc
     9841 tcgtctcctc tcccgatctg tcgccgccgc atcttccaaa tcggtgacga ctcgtgcctt
     9901 ttcaacggaa cttccatcga cgctcgattc cgctagtgat atcacaagtt tgtacaaaaa
     9961 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
    10021 aaaacagact acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta
    10081 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
    10141 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
    10201 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
    10261 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
    10321 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
    10381 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
    10441 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
    10501 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
    10561 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
    10621 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
    10681 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
    10741 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
    10801 caacagtact gcgatgagtg gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa
    10861 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
    10921 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag
    10981 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
    11041 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
    11101 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
    11161 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
    11221 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
    11281 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
    11341 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
    11401 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
    11461 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
    11521 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
    11581 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
    11641 aaagtggtga tatcccgcgg ccatgctaga gtccgcaaaa atcaccagtc tctctctaca
    11701 aatctatctc tctctatttt tctccagaat aatgtgtgag tagttcccag ataagggaat
    11761 tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt
    11821 tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtga
    11881 cctgcaggca tgcgac
//
