LOCUS       sequence-320775-        9229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of Flag-Polß(TM-K244A: L301R/V303R/V306R-K244A)
            portein with puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Fang Q, Inanc B, Schamus S, Wang XH, Wei L, Brown AR, Svilar D, 
            Sugrue KF, Goellner EM, Zeng X, Yates NA, Lan L, Vens C, Sobol RW
  TITLE     HSP90 regulates DNA repair via the interaction between XRCC1 and DNA
            polymerase beta.
  JOURNAL   Nat Commun. 2014 Nov 26;5:5513. doi: 10.1038/ncomms6513.
  PUBMED    25423885
REFERENCE   2  (bases 1 to 9229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-11-26"; volume: "5:5513. doi"; pages: " 
            10.1038/ncomms6513"
COMMENT     Sequence Label: pLVX-Flag-Polß(TM-K244A)-IRES-puro
FEATURES             Location/Qualifiers
     source          1..9229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    516..641
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1138..1371
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1556..1600
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1863..1978
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2036..2339
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2340..2543
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2494..2514
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2540..2564
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2659..2683
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2707..2730
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(3758..3782)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(3814..4388,4389..4391,4392..4400)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3981..3998)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4208..4227
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4420..5019
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4420..4439)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4916..4936
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5033..5621
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5086..5106)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5504..5515)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5828..6461
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6589..6605)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6589..6605)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6602..6624)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6613..6629
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6637..6643,6644..6661,6662..6667))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6682..6703
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6820..6837)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6991..7579)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7071..7090)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7750..8541,8542..8610))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8373..8392
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8611..8715)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8763..8897
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8800..8819)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8854..8873
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(9065..9229,1..469)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
       61 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
      121 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
      181 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
      241 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
      301 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      361 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      421 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      481 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      541 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      601 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      661 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      721 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      781 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      841 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
      901 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
      961 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     1021 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1081 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1141 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1201 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1261 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1321 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1381 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1441 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1501 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1561 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1621 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1681 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1741 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1801 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1861 cctttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1921 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     1981 gggtttatta cagggacagc agagatccag tttatcgata agcttgggag ttccgcgtta
     2041 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     2101 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     2161 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     2221 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     2281 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     2341 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     2401 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     2461 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     2521 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
     2581 cacgctgttt tgacctccat agaagacacc gactctacta gaggatctat ttccggtgaa
     2641 ttcctcgaga ctagatcaac aagtttgtac aaaaaagcag gctccgcggc cgcccccttc
     2701 accatggact acaaagacga tgacgataaa ggcatgagca aacggaaggc gccgcaggag
     2761 actctcaacg ggggaatcac cgacatgctc acagaactcg caaactttga gaagaacgtg
     2821 agccaagcta tccacaagta caatgcttac agaaaagcag catctgttat agcaaaatac
     2881 ccacacaaaa taaagagtgg agctgaagct aagaaattgc ctggagtagg aacaaaaatt
     2941 gctgaaaaga ttgatgagtt tttagcaact ggaaaattac gtaaactgga aaagattcgg
     3001 caggatgata cgagttcatc catcaatttc ctgactcgag ttagtggcat tggtccatct
     3061 gctgcaagga agtttgtaga tgaaggaatt aaaacactag aagatctcag aaaaaatgaa
     3121 gataaattga accatcatca gcgaattggg ctgaaatatt ttggggactt tgaaaaaaga
     3181 attcctcgtg aagagatgtt acaaatgcaa gatattgtac taaatgaagt taaaaaagtg
     3241 gattctgaat acattgctac agtctgtggc agtttcagaa gaggtgcaga gtccagtggt
     3301 gacatggatg ttctcctgac ccatcccagc ttcacttcag aatcaaccaa acagccaaaa
     3361 ctgttacatc aggttgtgga gcagttacaa aaggttcatt ttatcacaga taccctgtca
     3421 aagggtgaga caaagttcat gggtgtttgc cagcttccca gtgcaaatga tgaaaaagaa
     3481 tatccacaca gaagaattga tatcaggttg atacccaaag atcagtatta ctgtggtgtt
     3541 ctctatttca ctgggagtga tattttcaat aagaatatga gggctcatgc cctagaaaag
     3601 ggtttcacaa tcaatgagta caccatccgt ccccggggac gcactggacg tgcaggagaa
     3661 cccctgccag tggatagtga aaaagacatc tttgattaca tccagtggaa ataccgggaa
     3721 cccaaggacc ggagcgaatg aaagggtggg cgcgccgacc cagctttctt gtacaaagtg
     3781 gttgatctag ttctagagcg gccgcggatc ccgcccctct ccctcccccc cccctaacgt
     3841 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     3901 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     3961 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     4021 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     4081 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     4141 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     4201 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     4261 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     4321 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     4381 atgataagct tgccacaacc cacaaggaga cgaccttcca tgaccgagta caagcccacg
     4441 gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc
     4501 gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc
     4561 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     4621 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     4681 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     4741 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     4801 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     4861 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     4921 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     4981 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctaga cgcgtctgga acaatcaacc
     5041 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     5101 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     5161 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     5221 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     5281 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     5341 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     5401 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     5461 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     5521 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     5581 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt
     5641 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     5701 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     5761 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     5821 aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     5881 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
     5941 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
     6001 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     6061 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     6121 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
     6181 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
     6241 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
     6301 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6361 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6421 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     6481 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca
     6541 ttgctagcgt ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     6601 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6661 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6721 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6781 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6841 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6901 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6961 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7021 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7081 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7141 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7201 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7261 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7321 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7381 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     7441 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7501 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7561 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7621 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7681 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7741 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7801 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7861 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7921 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7981 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     8041 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     8101 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     8161 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     8221 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8281 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8341 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     8401 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8461 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8521 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     8581 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     8641 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8701 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     8761 tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8821 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8881 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
     8941 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
     9001 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
     9061 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
     9121 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
     9181 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagata
//
