LOCUS       sequence-320776-        9404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of Flag-Polß(TM-KTM: 
            L301R/V303R/V306R-K41R/K61R/K81R) protein with neomycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9404)
  AUTHORS   Fang Q, Inanc B, Schamus S, Wang XH, Wei L, Brown AR, Svilar D, 
            Sugrue KF, Goellner EM, Zeng X, Yates NA, Lan L, Vens C, Sobol RW
  TITLE     HSP90 regulates DNA repair via the interaction between XRCC1 and DNA
            polymerase beta.
  JOURNAL   Nat Commun. 2014 Nov 26;5:5513. doi: 10.1038/ncomms6513.
  PUBMED    25423885
REFERENCE   2  (bases 1 to 9404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-11-26"; volume: "5:5513. doi"; pages: " 
            10.1038/ncomms6513"
COMMENT     Sequence Label: pLVX-Flag-Polß(TM-KTM)-IRES-neo
FEATURES             Location/Qualifiers
     source          1..9404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(8..582,583..585,586..594)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(175..192)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     402..421
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             595..1389
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(649..668)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1259..1278
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    1403..1991
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1456..1476)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1874..1885)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2198..2831
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2959..2975)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2959..2975)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2972..2994)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2983..2999
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3007..3013,3014..3031,3032..3037))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3052..3073
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3190..3207)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3361..3949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3441..3460)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4120..4911,4912..4980))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4743..4762
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4981..5085)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    5133..5267
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5170..5189)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5224..5243
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5435..6068
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6115..6240
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6737..6970
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7155..7199
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7462..7577
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        7635..7938
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7939..8142
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8093..8113
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8139..8163
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    8257..8281
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8305..8328
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(9356..9380)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gatcccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
       61 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
      121 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
      181 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
      241 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
      301 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
      361 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
      421 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
      481 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
      541 accacgggga cgtggttttc ctttgaaaaa cacgatgata agcttgccac aaccatggct
      601 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
      661 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
      721 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
      781 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
      841 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
      901 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
      961 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     1021 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     1081 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     1141 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     1201 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     1261 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     1321 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     1381 ttcttctgaa cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac
     1441 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     1501 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     1561 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     1621 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     1681 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     1741 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     1801 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     1861 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     1921 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     1981 cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca
     2041 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     2101 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     2161 gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa
     2221 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
     2281 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
     2341 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
     2401 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
     2461 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
     2521 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
     2581 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
     2641 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     2701 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     2761 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     2821 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     2881 atgaatatca gagagtgaga ggccttgaca ttgctagcgt ttaccgtcga cctctagcta
     2941 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     3001 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     3061 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     3121 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     3181 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3241 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3301 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3361 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3421 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3481 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3541 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3601 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3661 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3721 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3781 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     3841 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3901 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3961 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4021 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4081 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     4141 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4201 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4261 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     4321 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4381 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     4441 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     4501 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4561 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     4621 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4681 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     4741 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     4801 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     4861 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     4921 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     4981 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     5041 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     5101 agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt
     5161 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5221 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc
     5281 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
     5341 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
     5401 gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga
     5461 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag
     5521 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
     5581 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta
     5641 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt
     5701 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac
     5761 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc
     5821 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
     5881 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     5941 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     6001 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     6061 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     6121 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     6181 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     6241 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     6301 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     6361 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     6421 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     6481 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     6541 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac
     6601 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     6661 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     6721 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     6781 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     6841 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     6901 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     6961 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     7021 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     7081 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     7141 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     7201 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     7261 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     7321 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     7381 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     7441 acggatctcg acggtatcgc ctttaaaaga aaagggggga ttggggggta cagtgcaggg
     7501 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     7561 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttatcgataa
     7621 gcttgggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     7681 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     7741 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     7801 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     7861 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     7921 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     7981 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     8041 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     8101 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
     8161 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg actctactag
     8221 aggatctatt tccggtgaat tcctcgagac taatcaacaa gtttgtacaa aaaagcaggc
     8281 tccgcggccg cccccttcac catggactac aaagacgatg acgataaagg catgagcaaa
     8341 cggaaggcgc cgcaggagac tctcaacggg ggaatcaccg acatgctcac agaactcgca
     8401 aactttgaga agaacgtgag ccaagctatc cacaagtaca atgcttacag aagagcagca
     8461 tctgttatag caaaataccc acacaaaata aagagtggag ctgaagctaa gagattgcct
     8521 ggagtaggaa caaaaattgc tgaaaagatt gatgagtttt tagcaactgg aagattacgt
     8581 aaactggaaa agattcggca ggatgatacg agttcatcca tcaatttcct gactcgagtt
     8641 agtggcattg gtccatctgc tgcaaggaag tttgtagatg aaggaattaa aacactagaa
     8701 gatctcagaa aaaatgaaga taaattgaac catcatcagc gaattgggct gaaatatttt
     8761 ggggactttg aaaaaagaat tcctcgtgaa gagatgttac aaatgcaaga tattgtacta
     8821 aatgaagtta aaaaagtgga ttctgaatac attgctacag tctgtggcag tttcagaaga
     8881 ggtgcagagt ccagtggtga catggatgtt ctcctgaccc atcccagctt cacttcagaa
     8941 tcaaccaaac agccaaaact gttacatcag gttgtggagc agttacaaaa ggttcatttt
     9001 atcacagata ccctgtcaaa gggtgagaca aagttcatgg gtgtttgcca gcttcccagt
     9061 aaaaatgatg aaaaagaata tccacacaga agaattgata tcaggttgat acccaaagat
     9121 cagtattact gtggtgttct ctatttcact gggagtgata ttttcaataa gaatatgagg
     9181 gctcatgccc tagaaaaggg tttcacaatc aatgagtaca ccatccgtcc ccggggacgc
     9241 actggacgtg caggagaacc cctgccagtg gatagtgaaa aagacatctt tgattacatc
     9301 cagtggaaat accgggaacc caaggaccgg agcgaatgaa agggtgggcg cgccgaccca
     9361 gctttcttgt acaaagtggt tgatctagtt ctagagcggc cgcg
//
