LOCUS       sequence-320791-        7285 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Blue-light dependent heterodimerization system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7285)
  AUTHORS   Benedetti L, Marvin JS, Falahati H, Guillen-Samander A, Looger LL, 
            De Camilli P
  TITLE     Optimized Vivid-derived Magnets photodimerizers for subcellular 
            optogenetics in mammalian cells.
  JOURNAL   Elife. 2020 Nov 11;9. pii: 63230. doi: 10.7554/eLife.63230.
  PUBMED    33174843
REFERENCE   2  (bases 1 to 7285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-11-11"; volume: "9. pii: 63230. doi"; pages: " 
            10.7554/eLife.63230"
COMMENT     Sequence Label: ER-EGFP-eMagA
FEATURES             Location/Qualifiers
     source          1..7285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1780..1789
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1930..1932,1933..1935,1936..2646)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     intron          3207..3339
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3322..3341
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    3348..3936
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3401..3421)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3819..3830)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3963..3980)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3969..4080
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4176..4196)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4176..4194)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4307..4326)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4372..4427
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4426..4445)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4788..4804)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4801..4823)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4812..4828
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4836..4842,4843..4860,4861..4866))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4881..4902
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4961..5156
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5007..5142
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5162..5296
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5199..5218)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5253..5272
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5364..5381)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5535..6123)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5615..5634)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6294..7085,7086..7154))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6917..6936
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7155..7259)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggggcgc gccggggggg gggggggggg gggggggggg
      541 gggggggggg gggggggggg ggggggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg ggggcggcag gggggggggc cgggcggggc
     1261 ggggccgcct cgggccgggg ggggctcggg gggggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcggtaccgc
     1741 gggcccggga tccaccggaa gccacctgca tgcggccgcg ccaccatgga tcctgtggtg
     1801 gtgctggggc tctgtctctc ctgtttgctt ctcctttcac tctggaaaca gagctatggg
     1861 ggagggttgg atccaccggt cggcggctcc ggaggcagcg gcggttctgg aggttccggt
     1921 ggcggctcca tggtgtcgaa gggagaggag cttttcaccg gcgtggtgcc gattcttgtg
     1981 gaactggacg gcgacgtgaa cggacataag ttcagcgtgt caggagaagg agaaggggac
     2041 gcgacttacg gaaagctgac cctgaagttc atctgtacca ccgggaagct cccggtgccc
     2101 tggccgaccc tcgtgactac tctgacctac ggagtgcagt gcttctcccg ctacccggac
     2161 catatgaagc agcacgattt cttcaagagc gctatgcccg agggttacgt gcaagaacgg
     2221 accatcttct tcaaggacga cggaaactac aagaccagag ccgaagtgaa attcgaagga
     2281 gacaccctcg tcaatcgcat cgagctgaag ggaatcgact ttaaggaaga tgggaatatc
     2341 ctgggtcaca agctggagta caactacaac tcccacaatg tgtacatcat ggcggataag
     2401 caaaagaacg gcattaaggt caacttcaag atccggcata acatagaaga tggctccgtg
     2461 caactggcgg accactacca gcagaacacc cccattgggg acggtccggt gctgctccct
     2521 gataaccact acctgtcaac ccaaagcgca ctgtccaagg accccaacga gaagagggac
     2581 cacatggtgc tgctggagtt cgtcactgcc gctggcatca ctctcggaat ggacgagctg
     2641 tacaaggggg gcggctccgg aggcagcggc ggttctggag gttccggtgg cggctccacg
     2701 cgtatgggac acactcttta cgcccctgga ggatacgaca ttatgggata tttggatcag
     2761 attgcgaacc gcccaaaccc tcaggtcgaa ctggggcctg tggacctgtc atgtgccctg
     2821 atcctgtgcg atctgaagca aaaggacact ccgatcgtct acgcctcgga agccttcttg
     2881 gagatgaccg gatacaacag acatgaggtg ctcggcagga actgcagatt cctgcagtcc
     2941 cccgacggga tggtgaaacc aaagtcgact cgcaaatatg tggactcgaa cacgatctac
     3001 accatcaaga aggccatcga ccggaacgcc gaggtccagg tggaggtggt caactttaag
     3061 aagaacggcc agcggttcgt gaactttctg accatcattc cggtccggga tgaaaccgga
     3121 gagtacagat actccatcgg attccagtgc gaaaccgaat gagttaacct cgaggtaccg
     3181 atgaatagct aaggtcgagg ccgcaggtaa gtatcaaggt tacaagacag gtttaaggag
     3241 accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta
     3301 ttggtcttac tgacatccac tttgcctttc tctccacagg tgtcgacaat caacctctgg
     3361 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     3421 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     3481 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     3541 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     3601 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     3661 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     3721 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     3781 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     3841 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     3901 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattcgagct cggtacgatc
     3961 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     4021 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     4081 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     4141 ggaggattgg gaagacaata gcccagcttt tgttcccttt agtgagggtt aattgcgcgc
     4201 ttggcgtaat catggtcata gctgtttcca ctcctcaggt gcaggctgcc tatcagaagg
     4261 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg
     4321 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
     4381 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     4441 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
     4501 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
     4561 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
     4621 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
     4681 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
     4741 tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt
     4801 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     4861 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     4921 cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa
     4981 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     5041 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     5101 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     5161 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5221 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5281 ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg
     5341 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5401 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5461 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5521 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5581 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     5641 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     5701 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     5761 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     5821 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     5881 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     5941 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     6001 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6061 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6121 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6181 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6241 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     6301 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     6361 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     6421 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     6481 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     6541 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     6601 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     6661 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     6721 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     6781 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     6841 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     6901 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     6961 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7021 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7081 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7141 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7201 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7261 acatttcccc gaaaagtgcc acctg
//
