LOCUS       sequence-320797-        8825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for stable expression of UL32 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8825)
  AUTHORS   Didychuk AL, Gates SN, Gardner MR, Strong LM, Martin A, Glaunsinger 
            BA
  TITLE     A pentameric protein ring with novel architecture is required for 
            herpesviral packaging.
  JOURNAL   Elife. 2021 Feb 8;10. pii: 62261. doi: 10.7554/eLife.62261.
  PUBMED    33554858
REFERENCE   2  (bases 1 to 8825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-02-8"; volume: "10. pii: 62261. doi"; pages: " 
            10.7554/eLife.62261"
COMMENT     Sequence Label: pLJM1-zeo-UL32
FEATURES             Location/Qualifiers
     source          1..8825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..334
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    2202..2319
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2368..2878
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2797..2815
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2887..3261
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     LTR             3333..3566
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3638..3759
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3675..3694)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3729..3748
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3799..3934
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3954..3973)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3955..3973)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3983..4000)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3983..3999)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3992..4014)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4141..4596
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4228..4247)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4438..4459
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4622..4726
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4727..4795,4796..5587)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4945..4964)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5758..6346
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6247..6266
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6500..6517
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6634..6655
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6670..6675,6676..6693,6694..6700)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6708..6724
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6713..6735
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6732..6748
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6732..6748
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6767..6787
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6769..6787
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6815..7041
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7042..7222
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7269..7394
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7887..8120
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8305..8349
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     8647..8667
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      361 gcgctaccgg atggcaactt cgccccccgg ggtcctggcg agcgtcgcgg tgtgcgaaga
      421 gtcccccggc agcagctgga aggcgggggc gttcgagcgc gcgtatgtgg ccttcgatcc
      481 cagcctcctg gctcttaacg aggctctctg cgccgagctc ctggcggcca gtcacgtgat
      541 aggtgtgccg cccgtcggta cgctcgacga ggacgtcgcc gccgacgtgg tcaccgcccc
      601 ctcaagggcc cgcggggggg cgggcgacgg agggggttcg ggcgggcgcg gaggaccccg
      661 caacccgccc ccggatccct gtggggaggg gcttttggac accgggccct tttccgcggc
      721 ggccatcgac accttcgcgc tggaccggcc ctgtctggtc tgccgcacca tcgagctgta
      781 caagcaaacc taccgcctct cgccccagtg ggtggccgat tacgcgtttc tctgtgccaa
      841 atgcctgggg gcgccccact gcgccgctag catcttcgtg gccgcgttcg agtttgtgta
      901 cgtcatggac cgccactttc tgcgcaccaa gaaggcgacc ctggtcggct ccttcgcacg
      961 ctttgccctc accataaacg acatccaccg gcactttttt ctccactgct gcttccgtac
     1021 cgacggcggg gtgcccgggc ggcatgccca gaagcaaccc aagccctcgc cctccccggg
     1081 agccgccaag gtgcagtact ccaactactc gttcctggcg cagtctgcca cccgggccct
     1141 catcggaacc ttggcctccg ggggcgagga gggggcgggg tcggcggcgg gatccggcac
     1201 gcagccgtcg ctcaccaccg cgctgatgaa ctggaaggat tgcgcccgtc tcctggactg
     1261 cacggagggt cggcgcgggg gcggggacag ctgctgtacg cgcgccgccg ctcgcaacgg
     1321 ggaattcgag acggtcgccg gggaccgcga gccggaggag tcgccggata cgtgggcgta
     1381 cgcggatctg gtcctgctgc tcctcgccgg cacccccgcc gtctgggagt cggggcccca
     1441 gctgcgcgct gccgcggagg cccgtcgcgc cacggtccgc cagtcctggg aagcgcatcg
     1501 cggggcacgc acgcgcgacg tggctccgcg ctttgcgcag tttaccgaac ccgatgccca
     1561 gcccgacctc gacctgggcc ctctgatggc caccgtgctg aaacacggcc gggggcgcgg
     1621 gcgcaccggc ggagaatgcc tgctgtgtaa cctgttactg gtgcgggctt actggctggc
     1681 cctgcgtaga ctccgggcct ccgtggtccg ctactcggaa aacaacacga gcctcttcga
     1741 ctgtatcgta cccgtcgtcg accagctcga ggcggacccc gagacgcagc ccggggacgg
     1801 gggccggttt gtgagcctgc tgcgggccgc ggggcccgag gccatcttta agcacatgtt
     1861 ctgtgacccc atgtgcgcca tcacggagat ggaggttgac ccctgggttc tgttcggcca
     1921 tccccccgcc acccatcccg acgagctgct gctccacaag gccaagctgg cctgcgggaa
     1981 cgagttcgag gggcgtgtct gcatagcgtt gcgtgccctc atctacacct tcaagacgta
     2041 tcaggtattt gtaccaaagc ccaccgcgct cgccacattt gtccgtgagg cgggcgcgct
     2101 gcttagacgc cactcgatct cgctcctgtc cctggagcac accctgtgta cctatgtatg
     2161 aaattctcga cctcgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
     2221 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     2281 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     2341 agagatccac tttggccgcg gctcgagggg gttggggttg cgccttttcc aaggcagccc
     2401 tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc gggaaacgca gcggcgccga
     2461 ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc acccggatct tcgccgctac
     2521 ccttgtgggc cccccggcga cgcttcctgc tccgccccta agtcgggaag gttccttgcg
     2581 gttcgcggcg tgccggacgt gacaaacgga agccgcacgt ctcactagta ccctcgcaga
     2641 cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg
     2701 ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg
     2761 tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc
     2821 ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc accgacctct ctccccaggg
     2881 ggatccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga
     2941 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc
     3001 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg
     3061 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg
     3121 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc
     3181 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc
     3241 gtggccgagg agcaggactg aggtaccttt aagaccaatg acttacaagg cagctgtaga
     3301 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     3361 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     3421 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     3481 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     3541 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     3601 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     3661 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     3721 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     3781 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     3841 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     3901 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     3961 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     4021 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     4081 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     4141 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4201 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4261 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     4321 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     4381 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4441 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     4501 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4561 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     4621 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     4681 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     4741 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     4801 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     4861 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     4921 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     4981 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5041 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5101 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5161 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5221 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5281 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5341 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5401 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5461 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5521 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5581 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     5641 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     5701 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5761 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5821 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5881 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     5941 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6001 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6061 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6121 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6181 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6241 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6301 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6361 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6421 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6481 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     6541 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     6601 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     6661 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     6721 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     6781 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     6841 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     6901 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
     6961 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
     7021 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
     7081 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7141 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7201 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     7261 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     7321 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     7381 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     7441 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     7501 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     7561 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     7621 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     7681 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     7741 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     7801 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     7861 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     7921 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     7981 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     8041 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     8101 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     8161 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     8221 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     8281 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     8341 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     8401 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     8461 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     8521 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     8581 cgattagtga acggatctcg acggtatcga tcacgagact agcctcgagc ggccgccccc
     8641 ttcaccgagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt
     8701 agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
     8761 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
     8821 tatca
//
