LOCUS       sequence-32080-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  TITLE     Other Lawson Lab plasmids
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 458 p5E-uasE1b
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    24..119
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        121..139
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     164..180
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     165..181
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(531..547)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(531..547)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(584..602)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    603..726
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(799..817)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(822..838)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(822..838)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gagttacgct accatggagc tccaaataat gattttattt tgactgatag tgacctgttc
       61 gttgcaacaa attgataagc aatgcttttt tataatgcca actttgtata gaaaagttgg
      121 taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt cgacggtatc
      181 gataagctta ggcctccaag gcggagtact gtcctccggg ctggcggagt actgtcctcc
      241 ggcaaggtcg gagtactgtc ctccgacact agaggtcgga gtactgtcct ccgacgcaag
      301 gcggagtact gtcctccggg ctgcggagta ctgtcctccg gcaaggtcgg agtactgtcc
      361 tccgacacta gaggtcggag tactgtcctc cgacgcaagg cggagtactg tcctccgggc
      421 tggcggagta ctgtcctccg gcaagggtcg actctagagg gtatataatg gatcccatcg
      481 cgtctcagcc tcactttgag ctcctccaca cgaattcctg cagcccgggg gatccactag
      541 ttctagagcg gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa
      601 ttcaagtttg tacaaaaaag ttgaacgaga aacgtaaaat gatataaata tcaatatatt
      661 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatgcagtc
      721 actatgaacc aactacttag atggtattag tgacctgtag aattcgagct ctagagctgc
      781 agggcggccg cgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc
      841 agctctgg
//
