LOCUS       sequence-320814-        9229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of Flag-Polß(D256A) protein with puromycin 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Fang Q, Inanc B, Schamus S, Wang XH, Wei L, Brown AR, Svilar D, 
            Sugrue KF, Goellner EM, Zeng X, Yates NA, Lan L, Vens C, Sobol RW
  TITLE     HSP90 regulates DNA repair via the interaction between XRCC1 and DNA
            polymerase beta.
  JOURNAL   Nat Commun. 2014 Nov 26;5:5513. doi: 10.1038/ncomms6513.
  PUBMED    25423885
REFERENCE   2  (bases 1 to 9229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-11-26"; volume: "5:5513. doi"; pages: " 
            10.1038/ncomms6513"
COMMENT     Sequence Label: pLVX-Flag-Polß(D256A)-IRES-puro
FEATURES             Location/Qualifiers
     source          1..9229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(8..582,583..585,586..594)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(175..192)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     402..421
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             614..1213
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(614..633)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1110..1130
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1227..1815
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1280..1300)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1698..1709)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2022..2655
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2783..2799)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2783..2799)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2796..2818)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2807..2823
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2831..2837,2838..2855,2856..2861))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2876..2897
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3014..3031)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3185..3773)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3265..3284)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3944..4735,4736..4804))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4567..4586
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4805..4909)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    4957..5091
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4994..5013)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5048..5067
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5259..5892
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5939..6064
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6561..6794
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6979..7023
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7286..7401
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        7459..7762
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7763..7966
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7917..7937
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7963..7987
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    8082..8106
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8130..8153
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(9181..9205)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gatcccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
       61 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
      121 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
      181 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
      241 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
      301 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
      361 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
      421 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
      481 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
      541 accacgggga cgtggttttc ctttgaaaaa cacgatgata agcttgccac aacccacaag
      601 gagacgacct tccatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
      661 cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
      721 cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
      781 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
      841 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
      901 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
      961 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     1021 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     1081 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     1141 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     1201 gcccggtgcc tagacgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat
     1261 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     1321 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     1381 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     1441 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     1501 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     1561 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     1621 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     1681 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     1741 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     1801 gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat
     1861 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     1921 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     1981 agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc
     2041 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     2101 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     2161 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     2221 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     2281 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     2341 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     2401 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     2461 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     2521 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     2581 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     2641 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     2701 agaaatgaat atcagagagt gagaggcctt gacattgcta gcgtttaccg tcgacctcta
     2761 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     2821 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     2881 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     2941 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     3001 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     3061 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     3121 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3181 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3241 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3301 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3361 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3421 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3481 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3541 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3601 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     3661 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3721 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3781 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     3841 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     3901 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     3961 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     4021 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     4081 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4141 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4201 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     4261 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4321 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4381 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4441 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4501 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4561 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4621 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4681 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     4741 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     4801 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     4861 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     4921 gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc agcttataat
     4981 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5041 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
     5101 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
     5161 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
     5221 aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca
     5281 aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta
     5341 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa
     5401 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt
     5461 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag
     5521 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa
     5581 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg
     5641 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt
     5701 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     5761 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     5821 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     5881 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     5941 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     6001 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     6061 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     6121 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     6181 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     6241 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     6301 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     6361 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc
     6421 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     6481 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     6541 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     6601 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     6661 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     6721 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     6781 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     6841 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     6901 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     6961 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     7021 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     7081 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     7141 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     7201 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     7261 gtgaacggat ctcgacggta tcgcctttaa aagaaaaggg gggattgggg ggtacagtgc
     7321 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     7381 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttatcg
     7441 ataagcttgg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     7501 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     7561 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     7621 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     7681 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     7741 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     7801 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     7861 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     7921 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
     7981 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta
     8041 ctagaggatc tatttccggt gaattcctcg agactagatc aacaagtttg tacaaaaaag
     8101 caggctccgc ggccgccccc ttcaccatgg actacaaaga cgatgacgat aaaggcatga
     8161 gcaaacggaa ggcgccgcag gagactctca acgggggaat caccgacatg ctcacagaac
     8221 tcgcaaactt tgagaagaac gtgagccaag ctatccacaa gtacaatgct tacagaaaag
     8281 cagcatctgt tatagcaaaa tacccacaca aaataaagag tggagctgaa gctaagaaat
     8341 tgcctggagt aggaacaaaa attgctgaaa agattgatga gtttttagca actggaaaat
     8401 tacgtaaact ggaaaagatt cggcaggatg atacgagttc atccatcaat ttcctgactc
     8461 gagttagtgg cattggtcca tctgctgcaa ggaagtttgt agatgaagga attaaaacac
     8521 tagaagatct cagaaaaaat gaagataaat tgaaccatca tcagcgaatt gggctgaaat
     8581 attttgggga ctttgaaaaa agaattcctc gtgaagagat gttacaaatg caagatattg
     8641 tactaaatga agttaaaaaa gtggattctg aatacattgc tacagtctgt ggcagtttca
     8701 gaagaggtgc agagtccagt ggtgacatgg atgttctcct gacccatccc agcttcactt
     8761 cagaatcaac caaacagcca aaactgttac atcaggttgt ggagcagtta caaaaggttc
     8821 attttatcac agataccctg tcaaagggtg agacaaagtt catgggtgtt tgccagcttc
     8881 ccagtaaaaa tgatgaaaaa gaatatccac acagaagaat tgctatcagg ttgataccca
     8941 aagatcagta ttactgtggt gttctctatt tcactgggag tgatattttc aataagaata
     9001 tgagggctca tgccctagaa aagggtttca caatcaatga gtacaccatc cgtcccttgg
     9061 gagtcactgg agttgcagga gaacccctgc cagtggatag tgaaaaagac atctttgatt
     9121 acatccagtg gaaataccgg gaacccaagg accggagcga atgaaagggt gggcgcgccg
     9181 acccagcttt cttgtacaaa gtggttgatc tagttctaga gcggccgcg
//
