LOCUS       sequence-320815-        9164 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of copGFP-Polß(L301R/V303R/V306R: TM) protein 
            with puromcyin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9164)
  AUTHORS   Fang Q, Inanc B, Schamus S, Wang XH, Wei L, Brown AR, Svilar D, 
            Sugrue KF, Goellner EM, Zeng X, Yates NA, Lan L, Vens C, Sobol RW
  TITLE     HSP90 regulates DNA repair via the interaction between XRCC1 and DNA
            polymerase beta.
  JOURNAL   Nat Commun. 2014 Nov 26;5:5513. doi: 10.1038/ncomms6513.
  PUBMED    25423885
REFERENCE   2  (bases 1 to 9164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-11-26"; volume: "5:5513. doi"; pages: " 
            10.1038/ncomms6513"
COMMENT     Sequence Label: pCT-CMV-copGFP-Polß(L301R/V303R/V306R)-puro
FEATURES             Location/Qualifiers
     source          1..9164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    104..337
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             522..566
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    833..949
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1039..1242
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1193..1213
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1239..1263
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1308..1317
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1338..2000
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             2079..2102
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        3144..3355
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3368..3636
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             3663..4262
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3663..3682)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4159..4179
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4269..4857
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4322..4342)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4740..4751)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4931..5164
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5236..5357
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5273..5292)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5327..5346
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5397..5532
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5565..5581)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5565..5581)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5578..5600)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5589..5605
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5613..5619,5620..5637,5638..5643))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5658..5679
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5796..5813)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5967..6555)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(6726..7517,7518..7586))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7349..7368
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7587..7691)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7759..7777
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7815..7837)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7937..7956
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8150..8172
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8164..8181
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8165..8181
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8196..8422
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8423..8603
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8650..8775
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
       61 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
      121 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
      181 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
      241 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
      301 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
      361 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
      421 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
      481 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
      541 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
      601 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
      661 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
      721 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
      781 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa
      841 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
      901 acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta tcgatactag
      961 tattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     1021 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     1081 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     1141 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     1201 ggcggtaggc gtgtacggtg ggaggtttat ataagcagag ctcgtttagt gaaccgtcag
     1261 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagattgcc accatggaga
     1321 gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc accctgaacg
     1381 gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc cgcatgacca
     1441 acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg agccacgtga
     1501 tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac cccttcctgc
     1561 acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag gacggcggcg
     1621 tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc gacttcaagg
     1681 tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc atccgcagca
     1741 acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc agcttcgccc
     1801 gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc cacatgcact
     1861 tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc gccttccgcc
     1921 gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag cacgccttca
     1981 agacccccat tgccttcgcc agatcccgcg ctcagtcgtc caattctgcc gtggacggca
     2041 ccgccggacc cggctccacc ggatctcgct ctagaatgga ctacaaagac gatgacgata
     2101 aaggcatgag caaacggaag gcgccgcagg agactctcaa cgggggaatc accgacatgc
     2161 tcacagaact cgcaaacttt gagaagaacg tgagccaagc tatccacaag tacaatgctt
     2221 acagaaaagc agcatctgtt atagcaaaat acccacacaa aataaagagt ggagctgaag
     2281 ctaagaaatt gcctggagta ggaacaaaaa ttgctgaaaa gattgatgag tttttagcaa
     2341 ctggaaaatt acgtaaactg gaaaagattc ggcaggatga tacgagttca tccatcaatt
     2401 tcctgactcg agttagtggc attggtccat ctgctgcaag gaagtttgta gatgaaggaa
     2461 ttaaaacact agaagatctc agaaaaaatg aagataaatt gaaccatcat cagcgaattg
     2521 ggctgaaata ttttggggac tttgaaaaaa gaattcctcg tgaagagatg ttacaaatgc
     2581 aagatattgt actaaatgaa gttaaaaaag tggattctga atacattgct acagtctgtg
     2641 gcagtttcag aagaggtgca gagtccagtg gtgacatgga tgttctcctg acccatccca
     2701 gcttcacttc agaatcaacc aaacagccaa aactgttaca tcaggttgtg gagcagttac
     2761 aaaaggttca ttttatcaca gataccctgt caaagggtga gacaaagttc atgggtgttt
     2821 gccagcttcc cagtaaaaat gatgaaaaag aatatccaca cagaagaatt gatatcaggt
     2881 tgatacccaa agatcagtat tactgtggtg ttctctattt cactgggagt gatattttca
     2941 ataagaatat gagggctcat gccctagaaa agggtttcac aatcaatgag tacaccatcc
     3001 gtccccgggg acgcactgga cgtgcaggag aacccctgcc agtggatagt gaaaaagaca
     3061 tctttgatta catccagtgg aaataccggg aacccaagga ccggagcgaa tgaggatctg
     3121 cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     3181 ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga
     3241 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     3301 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa
     3361 gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc
     3421 acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc
     3481 cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag
     3541 cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt
     3601 ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct
     3661 agatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     3721 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     3781 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     3841 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     3901 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     3961 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     4021 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     4081 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     4141 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     4201 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     4261 gagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     4321 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4381 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4441 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     4501 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     4561 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     4621 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     4681 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     4741 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     4801 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     4861 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     4921 aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct ttttgcttgt
     4981 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     5041 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     5101 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     5161 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     5221 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     5281 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5341 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     5401 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     5461 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     5521 tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata gctgtttcct
     5581 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     5641 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     5701 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     5761 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5821 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5881 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5941 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6001 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6061 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6121 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6181 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6241 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6301 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6361 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6421 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6481 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6541 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6601 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6661 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6721 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6781 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6841 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6901 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6961 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7021 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7081 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7141 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7201 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7261 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7321 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7381 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7441 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7501 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7561 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7621 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7681 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     7741 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     7801 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     7861 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     7921 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     7981 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
     8041 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     8101 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     8161 cgttgtaaaa cgacggccag tgccaagctg acgcgtgtag tcttatgcaa tactcttgta
     8221 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     8281 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     8341 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     8401 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     8461 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     8521 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     8581 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     8641 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     8701 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     8761 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     8821 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     8881 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     8941 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     9001 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     9061 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccact
     9121 gatcttcaga cctggaggag gagatatgag ggacaattgg agaa
//
