LOCUS       sequence-320818-       10216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing dCas9 and sgRNA containg MTSa targeting telomeres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10216)
  AUTHORS   Wu X, Mao S, Yang Y, Rushdi MN, Krueger CJ, Chen AK
  TITLE     A CRISPR/molecular beacon hybrid system for live-cell genomic 
            imaging.
  JOURNAL   Nucleic Acids Res. 2018 Jul 27;46(13):e80. doi: 10.1093/nar/gky304.
  PUBMED    29718399
REFERENCE   2  (bases 1 to 10216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-07-27"; volume: "46(13):e80. doi"; pages: "
            10.1093/nar/gky304"
COMMENT     Sequence Label: SL2-sgTelo-MTSa/EGFP/pdCas9-C1
FEATURES             Location/Qualifiers
     source          1..10216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        108..411
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        412..615
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    638..718
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      720..729
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(726..728,729..731,732..1445)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(771..792)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1032..1051)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1379..1400
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1568..1689
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1703..1951
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1703..1723
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1874..1893
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        2205..2508
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2509..2712
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             2752..2772
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2773..2796,2797..2799,2800..5286,5287..5289,
                     5290..6873)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6883..6903
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7100..7221
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(7228..7683)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7365..7386)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7577..7596
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7710..7814
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7816..8173
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7816..8007
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7839..7859)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8024..8159
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8208..9002
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8208..9002
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8262..8281)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8872..8891
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9190..9209)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9234..9281
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9610..10198
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10099..10118
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
       61 cctgattctg tggataaccg tattaccgcc atgcattagt tattacgcgt tacataactt
      121 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      181 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      241 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      301 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      361 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      421 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      481 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      541 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      601 tatataagca gagctggttt agtgaaccgt cagatccgct agcgctaccg gactcagatc
      661 tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg
      721 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
      781 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
      841 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
      901 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
      961 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     1021 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     1081 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     1141 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     1201 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     1261 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     1321 accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca
     1381 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     1441 agtaaagcgg ccgcgactct agatcataat cagccatacc acatttgtag aggttttact
     1501 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     1561 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     1621 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     1681 tgtatcttat taatagttat tagagggcct atttcccatg attccttcat atttgcatat
     1741 acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt
     1801 agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt
     1861 atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc
     1921 tttatatatc ttgtggaaag gacgaaacac cgttagggtt agggttaggg ttagtttgag
     1981 agctatgctg gaaacagcat agcaagttca aataaggcta gtccgttatc aacttggccc
     2041 cggagcagaa cgacaggagt tgtgtttgtg gacgaagagc ctgcagtctg ctccggggcc
     2101 agtggcaccg agtcggtgct ttttttagag acggagtcac tgccaaccga ttaatagtaa
     2161 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     2221 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     2281 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     2341 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     2401 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     2461 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     2521 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     2581 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     2641 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     2701 ataagcagag ctggtttagt gaaccgtcag atccgctagc ccaccatggt gcccaaaaag
     2761 aagaggaaag tggacaagaa gtattctatc ggactggcca tcgggactaa tagcgtcggg
     2821 tgggccgtga tcactgacga gtacaaggtg ccctctaaga agttcaaggt gctcgggaac
     2881 accgaccggc attccatcaa gaaaaatctg atcggagctc tcctctttga ttcaggggag
     2941 accgctgaag caacccgcct caagcggact gctagacggc ggtacaccag gaggaagaac
     3001 cggatttgtt accttcaaga gatattctcc aacgaaatgg caaaggtcga cgacagcttc
     3061 ttccataggc tggaagaatc attcctcgtg gaagaggata agaagcatga acggcatccc
     3121 atcttcggta atatcgtcga cgaggtggcc tatcacgaga aatacccaac catctaccat
     3181 cttcgcaaaa agctggtgga ctcaaccgac aaggcagacc tccggcttat ctacctggcc
     3241 ctggcccaca tgatcaagtt cagaggccac ttcctgatcg agggcgacct caatcctgac
     3301 aatagcgatg tggataaact gttcatccag ctggtgcaga cttacaacca gctctttgaa
     3361 gagaacccca tcaatgcaag cggagtcgat gccaaggcca ttctgtcagc ccggctgtca
     3421 aagagccgca gacttgagaa tcttatcgct cagctgccgg gtgaaaagaa aaatggactg
     3481 ttcgggaacc tgattgctct ttcacttggg ctgactccca atttcaagtc taatttcgac
     3541 ctggcagagg atgccaagct gcaactgtcc aaggacacct atgatgacga tctcgacaac
     3601 ctcctggccc agatcggtga ccaatacgcc gaccttttcc ttgctgctaa gaatctttct
     3661 gacgccatcc tgctgtctga cattctccgc gtgaacactg aaatcaccaa ggcccctctt
     3721 tcagcttcaa tgattaagcg gtatgatgag caccaccagg acctgaccct gcttaaggca
     3781 ctcgtccggc agcagcttcc ggagaagtac aaggaaatct tctttgacca gtcaaagaat
     3841 ggatacgccg gctacatcga cggaggtgcc tcccaagagg aattttataa gtttatcaaa
     3901 cctatccttg agaagatgga cggcaccgaa gagctcctcg tgaaactgaa tcgggaggat
     3961 ctgctgcgga agcagcgcac tttcgacaat gggagcattc cccaccagat ccatcttggg
     4021 gagcttcacg ccatccttcg gcgccaagag gacttctacc cctttcttaa ggacaacagg
     4081 gagaagattg agaaaattct cactttccgc atcccctact acgtgggacc cctcgccaga
     4141 ggaaatagcc ggtttgcttg gatgaccaga aagtcagaag aaactatcac tccctggaac
     4201 ttcgaagagg tggtggacaa gggagccagc gctcagtcat tcatcgaacg gatgactaac
     4261 ttcgataaga acctccccaa tgagaaggtc ctgccgaaac attccctgct ctacgagtac
     4321 tttaccgtgt acaacgagct gaccaaggtg aaatatgtca ccgaagggat gaggaagccc
     4381 gcattcctgt caggcgaaca aaagaaggca attgtggacc ttctgttcaa gaccaataga
     4441 aaggtgaccg tgaagcagct gaaggaggac tatttcaaga aaattgaatg cttcgactct
     4501 gtggagatta gcggggtcga agatcggttc aacgcaagcc tgggtaccta ccatgatctg
     4561 cttaagatca tcaaggacaa ggattttctg gacaatgagg agaacgagga catccttgag
     4621 gacattgtcc tgactctcac tctgttcgag gaccgggaaa tgatcgagga gaggcttaag
     4681 acctacgccc atctgttcga cgataaagtg atgaagcaac ttaaacggag aagatatacc
     4741 ggatggggac gccttagccg caaactcatc aacggaatcc gggacaaaca gagcggaaag
     4801 accattcttg atttccttaa gagcgacgga ttcgctaatc gcaacttcat gcaacttatc
     4861 catgatgatt ccctgacctt taaggaggac atccagaagg cccaagtgtc tggacaaggt
     4921 gactcactgc acgagcatat cgcaaatctg gctggttcac ccgctattaa gaagggtatt
     4981 ctccagaccg tgaaagtcgt ggacgagctg gtcaaggtga tgggtcgcca taaaccagag
     5041 aacattgtca tcgagatggc cagggaaaac cagactaccc agaagggaca gaagaacagc
     5101 agggagcgga tgaaaagaat tgaggaaggg attaaggagc tcgggtcaca gatccttaaa
     5161 gagcacccgg tggaaaacac ccagcttcag aatgagaagc tctatctgta ctaccttcaa
     5221 aatggacgcg atatgtatgt ggaccaagag cttgatatca acaggctctc agactacgac
     5281 gtggacgcca tcgtccctca gagcttcctc aaagacgact caattgacaa taaggtgctg
     5341 actcgctcag acaagaaccg gggaaagtca gataacgtgc cctcagagga agtcgtgaaa
     5401 aagatgaaga actattggcg ccagcttctg aacgcaaagc tgatcactca gcggaagttc
     5461 gacaatctca ctaaggctga gaggggcgga ctgagcgaac tggacaaagc aggattcatt
     5521 aaacggcaac ttgtggagac tcggcagatt actaaacatg tcgcccaaat ccttgactca
     5581 cgcatgaata ccaagtacga cgaaaacgac aaacttatcc gcgaggtgaa ggtgattacc
     5641 ctgaagtcca agctggtcag cgatttcaga aaggactttc aattctacaa agtgcgggag
     5701 atcaataact atcatcatgc tcatgacgca tatctgaatg ccgtggtggg aaccgccctg
     5761 atcaagaagt acccaaagct ggaaagcgag ttcgtgtacg gagactacaa ggtctacgac
     5821 gtgcgcaaga tgattgccaa atctgagcag gagatcggaa aggccaccgc aaagtacttc
     5881 ttctacagca acatcatgaa tttcttcaag accgaaatca cccttgcaaa cggtgagatc
     5941 cggaagaggc cgctcatcga gactaatggg gagactggcg aaatcgtgtg ggacaagggc
     6001 agagatttcg ctaccgtgcg caaagtgctt tctatgcctc aagtgaacat cgtgaagaaa
     6061 accgaggtgc aaaccggagg cttttctaag gaatcaatcc tccccaagcg caactccgac
     6121 aagctcattg caaggaagaa ggattgggac cctaagaagt acggcggatt cgattcacca
     6181 actgtggctt attctgtcct ggtcgtggct aaggtggaaa aaggaaagtc taagaagctc
     6241 aagagcgtga aggaactgct gggtatcacc attatggagc gcagctcctt cgagaagaac
     6301 ccaattgact ttctcgaagc caaaggttac aaggaagtca agaaggacct tatcatcaag
     6361 ctcccaaagt atagcctgtt cgaactggag aatgggcgga agcggatgct cgcctccgct
     6421 ggcgaacttc agaagggtaa tgagctggct ctcccctcca agtacgtgaa tttcctctac
     6481 cttgcaagcc attacgagaa gctgaagggg agccccgagg acaacgagca aaagcaactg
     6541 tttgtggagc agcataagca ttatctggac gagatcattg agcagatttc cgagttttct
     6601 aaacgcgtca ttctcgctga tgccaacctc gataaagtcc ttagcgcata caataagcac
     6661 agagacaaac caattcggga gcaggctgag aatatcatcc acctgttcac cctcaccaat
     6721 cttggtgccc ctgccgcatt caagtacttc gacaccacca tcgaccggaa acgctatacc
     6781 tccaccaaag aagtgctgga cgccaccctc atccaccaga gcatcaccgg actttacgaa
     6841 actcggattg acctctcaca gctcggaggg gatgagggag ctcccaagaa aaagcgcaag
     6901 gtaggtagtt ccgtgagcaa gggcgaggag ctgttctaag aattctgcag tcgacggtac
     6961 cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata ccacatttgt
     7021 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     7081 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     7141 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7201 caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg
     7261 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     7321 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     7381 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     7441 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     7501 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     7561 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     7621 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     7681 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7741 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7801 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     7861 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7921 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7981 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     8041 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     8101 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     8161 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     8221 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     8281 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     8341 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     8401 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     8461 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     8521 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     8581 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     8641 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     8701 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
     8761 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     8821 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     8881 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     8941 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     9001 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     9061 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     9121 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
     9181 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     9241 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
     9301 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
     9361 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     9421 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
     9481 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     9541 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     9601 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     9661 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     9721 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     9781 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     9841 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     9901 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     9961 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    10021 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    10081 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    10141 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    10201 cgccagcaac gcggcc
//
