LOCUS       sequence-320820-       10208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing dCas9 and sgRNA containg MTSa targeting telomeres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10208)
  AUTHORS   Wu X, Mao S, Yang Y, Rushdi MN, Krueger CJ, Chen AK
  TITLE     A CRISPR/molecular beacon hybrid system for live-cell genomic 
            imaging.
  JOURNAL   Nucleic Acids Res. 2018 Jul 27;46(13):e80. doi: 10.1093/nar/gky304.
  PUBMED    29718399
REFERENCE   2  (bases 1 to 10208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-07-27"; volume: "46(13):e80. doi"; pages: "
            10.1093/nar/gky304"
COMMENT     Sequence Label: SL2-sgTelo-MTSa/BFP/pdCas9-C1
FEATURES             Location/Qualifiers
     source          1..10208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(58..77)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    895..942
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     967..986
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(1174..1968)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(1174..1968)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1285..1304)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1895..1914
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2003..2360)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2017..2152
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        2169..2360
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     2317..2337
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(2362..2466)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2493..2948
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2580..2599)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2790..2811
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    2955..3076
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3273..3293)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(3303..4886,4887..4889,4890..7376,7377..7379,
                     7380..7403))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(7404..7424)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(7464..7667)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        7668..7971
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(8225..8473)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(8283..8302)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(8453..8473)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     polyA_signal    8487..8608
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    8732..8797
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(8798..9508)
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     regulatory      9505..9514
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9553..9756)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        9757..10060
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(join(10186..10208,1..566))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
       61 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      121 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
      181 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
      241 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
      301 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
      361 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
      421 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
      481 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
      541 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
      601 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
      661 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
      721 aacctgaggc tatggcaggg cctgccgccc cgacgttggc tgcgagccct gggccttcac
      781 ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg ggcgtattgg ccccaatggg
      841 gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa
      901 acgacccaac accgtgcgtt ttattctgtc tttttattgc cgtcatagcg cgggttcctt
      961 ccggtattgt ctccttccgt gtttcagtta gcctccccct agggtgggcg aagaactcca
     1021 gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc
     1081 ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg
     1141 cttggtcggt catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata
     1201 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
     1261 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
     1321 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
     1381 gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgct
     1441 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc
     1501 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc
     1561 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc
     1621 catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac
     1681 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca
     1741 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgca gttcattcag
     1801 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa
     1861 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc
     1921 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc
     1981 tcatcctgtc tcttgatcga tctttgcaaa agcctaggcc tccaaaaaag cctcctcact
     2041 acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta
     2101 gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag
     2161 ttaggggcgg gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
     2221 ctggggagcc tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca
     2281 tacttctgcc tgctggggag cctggggact ttccacaccc taactgacac acattccaca
     2341 gctggttctt tccgcctcag gactcttcct ttttcaatat tattgaagca tttatcaggg
     2401 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     2461 tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc
     2521 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     2581 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     2641 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     2701 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     2761 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     2821 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     2881 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     2941 tacaatttac gcgttaagat acattgatga gtttggacaa accacaacta gaatgcagtg
     3001 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     3061 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     3121 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
     3181 tcagttatct agatccggtg gatcccgggc ccgcggtacc gtcgactgca gaattcttag
     3241 aacagctcct cgcccttgct cacggaacta cctaccttgc gctttttctt gggagctccc
     3301 tcatcccctc cgagctgtga gaggtcaatc cgagtttcgt aaagtccggt gatgctctgg
     3361 tggatgaggg tggcgtccag cacttctttg gtggaggtat agcgtttccg gtcgatggtg
     3421 gtgtcgaagt acttgaatgc ggcaggggca ccaagattgg tgagggtgaa caggtggatg
     3481 atattctcag cctgctcccg aattggtttg tctctgtgct tattgtatgc gctaaggact
     3541 ttatcgaggt tggcatcagc gagaatgacg cgtttagaaa actcggaaat ctgctcaatg
     3601 atctcgtcca gataatgctt atgctgctcc acaaacagtt gcttttgctc gttgtcctcg
     3661 gggctcccct tcagcttctc gtaatggctt gcaaggtaga ggaaattcac gtacttggag
     3721 gggagagcca gctcattacc cttctgaagt tcgccagcgg aggcgagcat ccgcttccgc
     3781 ccattctcca gttcgaacag gctatacttt gggagcttga tgataaggtc cttcttgact
     3841 tccttgtaac ctttggcttc gagaaagtca attgggttct tctcgaagga gctgcgctcc
     3901 ataatggtga tacccagcag ttccttcacg ctcttgagct tcttagactt tcctttttcc
     3961 accttagcca cgaccaggac agaataagcc acagttggtg aatcgaatcc gccgtacttc
     4021 ttagggtccc aatccttctt ccttgcaatg agcttgtcgg agttgcgctt ggggaggatt
     4081 gattccttag aaaagcctcc ggtttgcacc tcggttttct tcacgatgtt cacttgaggc
     4141 atagaaagca ctttgcgcac ggtagcgaaa tctctgccct tgtcccacac gatttcgcca
     4201 gtctccccat tagtctcgat gagcggcctc ttccggatct caccgtttgc aagggtgatt
     4261 tcggtcttga agaaattcat gatgttgctg tagaagaagt actttgcggt ggcctttccg
     4321 atctcctgct cagatttggc aatcatcttg cgcacgtcgt agaccttgta gtctccgtac
     4381 acgaactcgc tttccagctt tgggtacttc ttgatcaggg cggttcccac cacggcattc
     4441 agatatgcgt catgagcatg atgatagtta ttgatctccc gcactttgta gaattgaaag
     4501 tcctttctga aatcgctgac cagcttggac ttcagggtaa tcaccttcac ctcgcggata
     4561 agtttgtcgt tttcgtcgta cttggtattc atgcgtgagt caaggatttg ggcgacatgt
     4621 ttagtaatct gccgagtctc cacaagttgc cgtttaatga atcctgcttt gtccagttcg
     4681 ctcagtccgc ccctctcagc cttagtgaga ttgtcgaact tccgctgagt gatcagcttt
     4741 gcgttcagaa gctggcgcca atagttcttc atctttttca cgacttcctc tgagggcacg
     4801 ttatctgact ttccccggtt cttgtctgag cgagtcagca ccttattgtc aattgagtcg
     4861 tctttgagga agctctgagg gacgatggcg tccacgtcgt agtctgagag cctgttgata
     4921 tcaagctctt ggtccacata catatcgcgt ccattttgaa ggtagtacag atagagcttc
     4981 tcattctgaa gctgggtgtt ttccaccggg tgctctttaa ggatctgtga cccgagctcc
     5041 ttaatccctt cctcaattct tttcatccgc tccctgctgt tcttctgtcc cttctgggta
     5101 gtctggtttt ccctggccat ctcgatgaca atgttctctg gtttatggcg acccatcacc
     5161 ttgaccagct cgtccacgac tttcacggtc tggagaatac ccttcttaat agcgggtgaa
     5221 ccagccagat ttgcgatatg ctcgtgcagt gagtcacctt gtccagacac ttgggccttc
     5281 tggatgtcct ccttaaaggt cagggaatca tcatggataa gttgcatgaa gttgcgatta
     5341 gcgaatccgt cgctcttaag gaaatcaaga atggtctttc cgctctgttt gtcccggatt
     5401 ccgttgatga gtttgcggct aaggcgtccc catccggtat atcttctccg tttaagttgc
     5461 ttcatcactt tatcgtcgaa cagatgggcg taggtcttaa gcctctcctc gatcatttcc
     5521 cggtcctcga acagagtgag agtcaggaca atgtcctcaa ggatgtcctc gttctcctca
     5581 ttgtccagaa aatccttgtc cttgatgatc ttaagcagat catggtaggt acccaggctt
     5641 gcgttgaacc gatcttcgac cccgctaatc tccacagagt cgaagcattc aattttcttg
     5701 aaatagtcct ccttcagctg cttcacggtc acctttctat tggtcttgaa cagaaggtcc
     5761 acaattgcct tcttttgttc gcctgacagg aatgcgggct tcctcatccc ttcggtgaca
     5821 tatttcacct tggtcagctc gttgtacacg gtaaagtact cgtagagcag ggaatgtttc
     5881 ggcaggacct tctcattggg gaggttctta tcgaagttag tcatccgttc gatgaatgac
     5941 tgagcgctgg ctcccttgtc caccacctct tcgaagttcc agggagtgat agtttcttct
     6001 gactttctgg tcatccaagc aaaccggcta tttcctctgg cgaggggtcc cacgtagtag
     6061 gggatgcgga aagtgagaat tttctcaatc ttctccctgt tgtccttaag aaaggggtag
     6121 aagtcctctt ggcgccgaag gatggcgtga agctccccaa gatggatctg gtggggaatg
     6181 ctcccattgt cgaaagtgcg ctgcttccgc agcagatcct cccgattcag tttcacgagg
     6241 agctcttcgg tgccgtccat cttctcaagg ataggtttga taaacttata aaattcctct
     6301 tgggaggcac ctccgtcgat gtagccggcg tatccattct ttgactggtc aaagaagatt
     6361 tccttgtact tctccggaag ctgctgccgg acgagtgcct taagcagggt caggtcctgg
     6421 tggtgctcat cataccgctt aatcattgaa gctgaaagag gggccttggt gatttcagtg
     6481 ttcacgcgga gaatgtcaga cagcaggatg gcgtcagaaa gattcttagc agcaaggaaa
     6541 aggtcggcgt attggtcacc gatctgggcc aggaggttgt cgagatcgtc atcataggtg
     6601 tccttggaca gttgcagctt ggcatcctct gccaggtcga aattagactt gaaattggga
     6661 gtcagcccaa gtgaaagagc aatcaggttc ccgaacagtc catttttctt ttcacccggc
     6721 agctgagcga taagattctc aagtctgcgg ctctttgaca gccgggctga cagaatggcc
     6781 ttggcatcga ctccgcttgc attgatgggg ttctcttcaa agagctggtt gtaagtctgc
     6841 accagctgga tgaacagttt atccacatcg ctattgtcag gattgaggtc gccctcgatc
     6901 aggaagtggc ctctgaactt gatcatgtgg gccagggcca ggtagataag ccggaggtct
     6961 gccttgtcgg ttgagtccac cagctttttg cgaagatggt agatggttgg gtatttctcg
     7021 tgataggcca cctcgtcgac gatattaccg aagatgggat gccgttcatg cttcttatcc
     7081 tcttccacga ggaatgattc ttccagccta tggaagaagc tgtcgtcgac ctttgccatt
     7141 tcgttggaga atatctcttg aaggtaacaa atccggttct tcctcctggt gtaccgccgt
     7201 ctagcagtcc gcttgaggcg ggttgcttca gcggtctccc ctgaatcaaa gaggagagct
     7261 ccgatcagat ttttcttgat ggaatgccgg tcggtgttcc cgagcacctt gaacttctta
     7321 gagggcacct tgtactcgtc agtgatcacg gcccacccga cgctattagt cccgatggcc
     7381 agtccgatag aatacttctt gtccactttc ctcttctttt tgggcaccat ggtgggctag
     7441 cggatctgac ggttcactaa accagctctg cttatataga cctcccaccg tacacgccta
     7501 ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt
     7561 tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag
     7621 tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat ggtaatagcg
     7681 atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca
     7741 taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga
     7801 tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa
     7861 tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg
     7921 gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc
     7981 atatatgggc tatgaactaa tgaccccgta attgattact attaatcggt tggcagtgac
     8041 tccgtctcta aaaaaagcac cgactcggtg ccactggccc cggagcagac tgcaggctct
     8101 tcgtccacaa acacaactcc tgtcgttctg ctccggggcc aagttgataa cggactagcc
     8161 ttatttgaac ttgctatgct gtttccagca tagctctcaa actaacccta accctaaccc
     8221 taacggtgtt tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag
     8281 ttacggtaag catatgatag tccattttaa aacataattt taaaactgca aactacccaa
     8341 gaaattatta ctttctacgt cacgtatttt gtactaatat ctttgtgttt acagtcaaat
     8401 taattccaat tatctctcta acagccttgt atcgtatatg caaatatgaa ggaatcatgg
     8461 gaaataggcc ctctaataac tattaataag atacattgat gagtttggac aaaccacaac
     8521 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     8581 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     8641 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
     8701 ggctgattat gatcagttat ctagatccgg tggatcccgg gcccgcggta ccgtcgactg
     8761 cagaattcga agcttgagct cgagatctga gtccggaatt aagcttgtgc cccagtttgc
     8821 tagggaggtc gcagtatctg gccactgcca cctcgtgctg ctcgacgtag gtctcgttgt
     8881 tggcctcctt gattctttcc agtctgtagt ccacatagta gacgccaggc atcttgaggt
     8941 tcttagcggg tttcttggat ctatatgtgg tcttggcgtt tgcgatcaga tggctcccgc
     9001 ccacgagctt cagggccatg tcgtttctgc cttccaggcc gccgtcagcg gggtacagcg
     9061 tctcggtgaa ggcctcccag ccgagtgttt tcttctgcat cacagggccg ttggatgtga
     9121 agttcacccc tctgatcttg acgttgtaga tgaggcagcc gtcctggagg ctggtgtcct
     9181 gggtagcggt cagcacgccc ccgtcttcgt atgtggtgac tctctcccat gtgaagccct
     9241 cagggaagga ctgcttgaag aagtcgggga tgccctgggt gtggttgatg aaggtcttgc
     9301 tgccgtagag gaagctagta gccaggatgt cgaaggcgaa ggggagaggg ccgccctcga
     9361 ccaccttgat tctcatggtc tgggtgccct cgtagggctt gccttcgccc tcggatgtgc
     9421 acttgaagtg atggttgtcc acggtgccct ccatgtacag cttcatgtgc atgttctcct
     9481 taatcagctc ttcgccctta gacaccatgg tggcgaccgg tagcgctagc ggatctgacg
     9541 gttcactaaa ccagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg
     9601 cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa
     9661 caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta
     9721 tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac
     9781 gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc
     9841 gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg
     9901 tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc
     9961 tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg
    10021 cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cgtaataact aatgcatggc
    10081 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    10141 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    10201 cccccctg
//
