LOCUS       sequence-320829-       14972 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SEC plasmid containing LG1 homology arms and 
            vha-8p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of 
            TIR1 and nuclear BFP reporter in C. elegans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14972)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 14972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pJH126
FEATURES             Location/Qualifiers
     source          1..14972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1903..1968
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             2824..2955
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             2956..2982
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     terminator      2991..3314
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(3347..3363)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3347..3363)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(4836..4856)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     rep_origin      complement(4993..5581)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5073..5092)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5752..6543,6544..6612))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6375..6394
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6613..6717)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6785..6803
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6841..6863)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    7186..7206
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(8713..8746)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(8746..8757)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(10179..10502)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    12420..12444
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             12447..13472
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             12447..13472
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(13473..13497)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(13850..13883)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     14252..14269
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14253..14269
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(14371..14382)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atgcaaaaga gaatcgcctt gtcgtttcca gaagaagtac tcgagcacgt cttctccttc
       61 atccaactcg acaaggaccg taactccgtc tccctcgtct gcaagtcctg gtacgagatc
      121 gagcgttggt gccgtcgtaa ggtcttcatc ggaaactgct acgccgtctc cccagccacc
      181 gtcatccgtc gtttcccaaa ggtccgttcc gtcgagctca agggaaagcc acacttcgcc
      241 gacttcaacc tcgtcccaga cggatgggga ggatacgtct acccatggat cgaggccatg
      301 tcctcctcct acacctggct cgaggagatc cgtctcaagc gtatggtcgt caccgacgac
      361 tgcctcgagc tcatcgccaa gtccttcaag aacttcaagg tcctcgtcct ctcctcctgc
      421 gagggattct ccaccgacgg actcgccgcc atcgccgcca cctgccgtaa cctcaaggta
      481 agtttaaaca tatatatact aactaaccct gattatttaa attttcagga gctcgacctc
      541 cgtgagtccg acgtcgacga ggtctccgga cactggctct cccacttccc agacacctac
      601 acctccctcg tctccctcaa catctcctgc ctcgcctccg aggtctcctt ctccgccctc
      661 gagcgtctcg tcacccgttg cccaaacctc aagtccctca agctcaaccg tgccgtccca
      721 ctcgagaagc tcgccaccct cctccaacgt gccccacaac tcgaggagct cggaaccgga
      781 ggatacaccg ccgaggtccg tccagacgtc tactccggac tctccgtcgc cctctccgga
      841 tgcaaggtaa gtttaaacag ttcggtacta actaaccata catatttaaa ttttcaggag
      901 ctccgttgcc tctccggatt ctgggacgcc gtcccagcct acctcccagc cgtctactcc
      961 gtctgctccc gtctcaccac cctcaacctc tcctacgcca ccgtccaatc ctacgacctc
     1021 gtcaagctcc tctgccaatg cccaaagctc caacgtctct gggtcctcga ctacatcgag
     1081 gacgccggac tcgaggtcct cgcctccacc tgcaaggacc tccgtgagct ccgtgtcttc
     1141 ccatccgagc cattcgtcat ggagccaaac gtcgccctca ccgagcaagg actcgtctcc
     1201 gtctccatgg gatgcccaaa gctcgagtcc gtcctctact tctgccgtca aatgaccaac
     1261 gccgccctca tcaccatcgc ccgtaaccgt ccaaacatga cccgtttccg tctctgcatc
     1321 atcgagccaa aggccccaga ctacctcacc ctcgagccac tcgacatcgg attcggagcc
     1381 atcgtcgagc actgcaagga cctccgtcgt ctctccctct ccggactcct caccgacaag
     1441 gtcttcgagt acatcggaac ctacgccaag aagatggaga tgctctccgt cgccttcgcc
     1501 ggagactccg acctcggact ccaccacgtc ctctccggat gcgactccct ccgtaagctc
     1561 gagatccgtg actgcccatt cggagacaag gccctcctcg ccaacgcctc caagctcgag
     1621 accatgcgtt ccctctggat gtcctcctgc tccgtctcct tcggagcctg caagctcctc
     1681 ggacaaaaga tgccaaagct caacgtcgag gtcatcgacg agcgtggagc cccagactcc
     1741 cgtccagagt cctgcccagt cgagcgtgtc ttcatctacc gtaccgtcgc cggaccacgt
     1801 ttcgacatgc caggattcgt ctggaacatg gaccaagact ccaccatgcg tttctcccgt
     1861 caaatcatca ccaccaacgg actcggagga ggaggatccc cagtcaagca aaccctcaac
     1921 ttcgacctcc tcaagctcgc cggagacgtc gagtccaacc caggaccaat ggtttcggaa
     1981 ttgattaagg aaaatatgca catgaagctc tacatggagg gaaccgtcga caaccaccac
     2041 ttcaagtgca cctccgaggg agagggaaag ccatacgagg gaacccaaac catgcgtatc
     2101 aaggtcgtcg agggaggacc actcccattc gccttcgaca tcctcgccac ctccttcctc
     2161 tacggatcca aggtaagttt aaacatatat atactaacta accctgatta tttaaatttt
     2221 cagaccttca tcaaccacac ccaaggaatc ccagacttct tcaagcaatc cttcccagag
     2281 ggattcacct gggagcgtgt caccacctac gaggacggag gagtcctcac cgccacccaa
     2341 gacacctccc tccaagacgg atgcctcatc tacaacgtca aggtaagttt aaacagttcg
     2401 gtactaacta accatacata tttaaatttt cagatccgtg gagtcaactt cacctccaac
     2461 ggaccagtca tgcaaaagaa gaccctcgga tgggaggcct tcaccgagac cctctaccca
     2521 gccgacggag gactcgaggg acgtaacgac atggccctca agctcgtcgg aggatcccac
     2581 ctcatcgcca acgccaaggt aagtttaaac atgattttac taactaacta atctgattta
     2641 aattttcaga ccacctaccg ttccaagaag ccagccaaga acctcaagat gccaggagtc
     2701 tactacgtcg actaccgtct cgagcgtatc aaggaggcca acaacgagac ctacgtcgag
     2761 caacacgagg tcgccgtcgc ccgttactgc gacctcccat ccaagctcgg acacaagctc
     2821 aaccctaaag atccagccaa acctccggcc aaggcacaag ttgtgggatg gccaccggtg
     2881 agatcatacc ggaagaacgt gatggtttcc tgccaaaaat caagcggtgg cccggaggcg
     2941 gcggcgttcg tgaaaccagc cgccaagcgt gtcaagcttg actaagataa atgcaaaatc
     3001 ctttcaagca ttcccttctt ctctatcact cttctttctt tttgtcaaaa aattctctcg
     3061 ctaatttatt tgctttttta atgttattat tttatgactt tttatagtca ctgaaaagtt
     3121 tgcatctgag tgaagtgaat gctatcaaaa tgtgattctg tctgatgtac tttcacaatc
     3181 tctcttcaat tccattttga agtgctttaa acccgaaagg ttgagaaaaa tgcgagcgct
     3241 caaatatttg tattgtgttc gttgagtgac ccaacaaaaa gaggaaactt tattgtgccg
     3301 ccaagaaaaa agtctcacgt acggtgcgcg cgatgcccta ggcatggtca tagctgtttc
     3361 ctgcgcaagt cggcgatttc tttgaagttt tgtcaaaaga agagacaagg acttgataga
     3421 tacgctcatc agagtgagat tcattgcaaa tagtgtgtaa atcgtggaga gacgagagaa
     3481 aaggtgtgag aagcttagaa gataaagaat aggaagaaga acatatctca tttcctattt
     3541 tctctaattt ttcgttcatt tttctagaca ttacccattt gatggttttt caatatggtt
     3601 atcagggtac tcattttggt agttttcaaa gcaatttgga gttttttttt aaattgtcaa
     3661 ctacaaaaac gcagcttaaa ggtccaaata tcatagtata aaaattcttt aaaaaattcc
     3721 aaattttata tgattttttg aaaattggaa gaatgttagt ttttgccatt tttttggaag
     3781 tttttctccg gattttatgg tacttatatt tcaaatataa atttttgtag gaattcgagt
     3841 atgcgagaga gctatagttt actaaaaaaa agtggcaaaa actcagtaat tgccactttt
     3901 ttcaaaaatg ttttgaaaaa ttttactatg atatttggcc attttggcac catatgtacg
     3961 ttacttttta cgaaacactc taatttttga aatttgagtt tcatgtttaa tcttttgtat
     4021 aaaattttta tggtcctttt gtgacaggat aaaaaattgc aaaaaaatag aaaactttgt
     4081 ttcgtgaaaa cacctagttt aaaaattgtg ggccacttag cgattctagt caaaaatcag
     4141 acaaatgtca gctgccgtaa ctttttccgg atcagggtta tcaaatttta attttaattg
     4201 taaatgtaga aaaatgatcg gcaacatttt gctaattgaa aatattttga aaattgctat
     4261 agtacatatt aatctagtat taatatagta ttaatgttat aatatcagtt atgtgtttga
     4321 ataactcaag cgtaagtttc cgtaaaatat ctttaaattt tggtaatttt ttacggtttg
     4381 gtataaattt ttgcattttt tgattttcta cacttttttt cgatttcgca ttttccgaat
     4441 ttctctgaaa taaatcgaac aatcggaaaa ttttcgaggg cacccttagc catgcagctc
     4501 aggaatttga ctttatgttc ccattcaaat ttttttaaaa ttaaataaaa tgctaattgt
     4561 aagataaatt acaaaaatta ttaaatctca tcatccttat gatgatagtg cagtgcatga
     4621 atatattttt ccaaatagga tatgattcgt cgagtagctt cacgtaatcc gttttcttca
     4681 atcaccattt cgaactgcaa tttttttttt ggatttttgt aacaatattg tcataaattg
     4741 tgattttgat attttgcgta gagcatcaga acctttctgc tttgaatttt aatgggatta
     4801 aaaattataa aaaacttgca ccctaattaa agcgccaact ttattataca tagttgataa
     4861 ttcgatggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     4921 cgcaggaaag aacatgtgag caaaaggcca gcaaaagacc aggaaccgta aaaaggccgc
     4981 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5041 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     5101 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     5161 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     5221 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     5281 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     5341 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     5401 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     5461 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     5521 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     5581 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     5641 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     5701 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     5761 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     5821 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctgacc ccagtgctgc
     5881 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     5941 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     6001 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     6061 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     6121 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     6181 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     6241 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     6301 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     6361 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     6421 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     6481 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     6541 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     6601 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     6661 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     6721 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     6781 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     6841 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     6901 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     6961 tgcggcatca gagcagattg cactgagagt gcaccatatg cggtgtgaaa taccgcacag
     7021 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
     7081 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     7141 tgcaaggcga ttaagttggg taacgccagg gatccgccaa gctatcaact ttgtatagaa
     7201 aagttgcaag tggggatcag gaagaagttg ataaagtgat tcaaaaactc gaggagctca
     7261 acacagcgat gaacccaatt ttgagagcaa cactgacgaa gaactatgac gcgagggtag
     7321 gcactacctt gaagctatcc acctgcctgc ctgccgccta tttccatgtg cctactgcgc
     7381 atggaaatag gcggtagtca ggcaggtaga tagcctcata aaattcaata cttccttttc
     7441 agaaagaatg ctggaatgta ttcaaagagg agtcactctt cccattgctc agagaatatg
     7501 aagagttgct cggcgagcaa aagttcatga ttggagatcg atattcatgg gctgacatcg
     7561 ctgtcgtcga gtttctaact cggtgtcagc agtgctacga tagcttctat ctggctcatt
     7621 tcgagaggct ccgaactttt tgccagactt ttgagtctct tccgcacatt cgtccctaca
     7681 ttcagggacg tgttgattca tttatctaat tttttaaaat ggatattttt cgttttttat
     7741 ttgtttgttt tgctgataat tcaataaagt tacgcaacga attttagaca tttatgaaaa
     7801 ccgaaaatcg acaaaaatat tttaagaaaa atagcagcaa aattcaatat tttatcaaat
     7861 tttgtttatg gtaatgagaa agaaaacata caatattttt ctaagaagaa gaaaaatgac
     7921 cagaatttta agatttttct cgaatatacg aaaaattgat aaactaaaat tttcgatttt
     7981 tccatttatt ttgaaaaaaa aactcttgac ggttttcacc atgcgaagag gtgagaaata
     8041 ttctgaaagt tatgcatttt gaaaaaattg caaaaaaata cgttaaaatt ttttgatcta
     8101 ttgttttttt ttgaaaaccg gaatttaaaa tggaattttt tttcaaaaat taaaattaaa
     8161 atttaaatat ttctagtgtt tggatagttt ttcaaaagct agatttaacc cgcgagatgt
     8221 cgaccgctag tgtagcttac agatggctga agacttcgca aaaatcttat cttagaaacc
     8281 cgcgaggtgt cggccgctaa aactttgtaa acctgccaac aaaatgcttc gcattgtctg
     8341 aacagatgaa accttgaaac aaaacatata caattgaaat cccgtaaagt gtcgggcgca
     8401 agaactgcga ttagttgtat aattggacta gctcaaattt gaccaaaatc tcatttttag
     8461 acctctaaga tgtcggccac tataacttta ttcaacttga aactgcaaaa aaacttatca
     8521 actgtataga ttcaagtatt atgtcaagta ttggttttag agctacagta cactctgata
     8581 acaaaatctg cagaaatacc tccctgtcaa ttcccaaaat acctcccctc atctcaatta
     8641 tcccgaaaat cggttagtca ttgccatcag aaaatcgaat taggtaccat gatggtagca
     8701 aactcacttc gtataacttc gtatagtata ccttatacga agttatctgc cctcgattgt
     8761 ctatcttcca ttctcaccaa gtttcaacaa ctttattcag tcattcttct ctggttcatt
     8821 tcccaaccaa tcttcccgaa taaagcccat ctgtcctttc ggccacattc ctcacctaca
     8881 tccgtactcc ttatctcccg ttcttatcca gtcttttatc gtctctgcgt ctcgcacact
     8941 gtctcgtcgg taatgtaaaa aaaaaacaaa aaaaacttga aaacttgcgg aaagatttcc
     9001 taataaaagg agacgaatcc ggtaggaaat cgacaagtct ctgcttctcg aagactaggt
     9061 gagttacatt tctttggccg aattattttg aaatcctaaa tttgataatt tcagagatgt
     9121 ctgtaaaact tgcgtgttat gtgacggctt cggtcactgt cgccactctt atggtatgct
     9181 tcatgaccat gtcaaccatc tactcggagg ttgatggatt cagagagaag cttgataccg
     9241 agatgaatgt gttcagagtg agttaaatta tattttgaat tttaaataat ttttaatttt
     9301 ctagcaatct accaatggat tgtggaagga catagttgtc atcggaagat ctagcaagcg
     9361 tgtccgttgt caatatgaag agaccaacgc taccccaact ccacatgctg atggatcccc
     9421 atctgctcca ccaggtcaac caccagcagt tccaccagtc ttcaaccagc caaagactcc
     9481 aaatggagcc aatggaaatg gaccaacctg caactgcaat gctgataaca agtgcccagc
     9541 tggaccatcc ggaccaaagg gagttccagg agttccagga ctcgacggag ttccaggact
     9601 tgacggtgtt ccaggagttg gagctgatga tatcgctcca caacgcgagt ctgtcggatg
     9661 cttcacttgc ccacaaggac cagttggacc accaggagct cttggaagac caggaccacg
     9721 tggacttcca ggaccaagag gacaaaatgg aaacccagga agagatggac aaccaggaca
     9781 tccaggagag caaggatcat ccggtcaaat cggaaagatc ggagagccag gaccaccagg
     9841 agagaaggga cgcgacgccg agcatccaat cggaagacca ggaccaaagg gaccaagagg
     9901 agatcaagga ccaacaggac cagctggaca gaacggtctt cacggaccac caggagagcc
     9961 aggaaccgtt ggaccagaag gaccatctgg aaagcaagga cgtcaaggac cagacggaac
    10021 ccagggagag actggaccag acggaagacc aggaaaggat gccgagtact gccagtgccc
    10081 agacaagtct ccaccatcag aggctgtcaa cgccaaccgt ggatacagaa atatctaaat
    10141 tgttggtgtt ttctaataaa atatttgaga ttcagtgaga cttttttctt ggcggcacaa
    10201 taaagtttcc tctttttgtt gggtcactca acgaacacaa tacaaatatt tgagcgctcg
    10261 catttttctc aacctttcgg gtttaaagca cttcaaaatg gaattgaaga gagattgtga
    10321 aagtacatca gacagaatca cattttgata gcattcactt cactcagatg caaacttttc
    10381 agtgactata aaaagtcata aaataataac attaaaaaag caaataaatt agcgagagaa
    10441 ttttttgaca aaaagaaaga atagtgatag agaagaaggg aatgcttgaa aggattttgc
    10501 atttatctta atcaccgtct tcgagaagac gaaccatggc gccagtttca gaatccaagt
    10561 ttctaatata gttcatcaca atgttgacgt tggtccaacc tccggcttgc ataatctctg
    10621 ggatggaaac tccagcgcga gccatgtcac gggcagctcc gacacgggcg gaatgtccag
    10681 accaagccaa gtaacgttgt ccggaatcat ccttggctcc gtagatgaga cggtgggtag
    10741 cttcgaagat tcctgaaaat ttattttcag cttattctta gctaatatgt tgtttaaact
    10801 taccttcaag agctcgagtc gagagttgtg atgttgcact cggggcagca actccgttct
    10861 tgcgaacacg acagaacagg tagttgtttg gatcatcggc gactccggaa accgaaatcc
    10921 agcgctcaac gagcttggta actccaaggg agagcgcttt ttcgactccg gcagtcgaga
    10981 ccagcgtttt tgtacgtccg atgtggatga gcattcttcc tccgtctgtg cgactaatat
    11041 ccttgacgcg aatgcgggca atttcggcga tacggagaag tgtgttgtaa gcaattccga
    11101 ggaaggcgag gtttcggatg tcctggcaac gatcggaatt ttccataaga gaacggacct
    11161 gatcgaagtc ggttctgaaa atttcgtcaa gtcttaggat tgaatacatt aatatttaaa
    11221 cttacctttc gaaggcgagg gcttgcttgg ctctctctcc agcatcaaca ttttccttgc
    11281 ggatacgtct cattacgaga gatacggcat tagaatctga tgggcgtgga agtccagatc
    11341 ggcgatggag catattgagc tgtccgagat gttgttggat cgtcttcacc gccaatccac
    11401 gagcttggag gtagagcaag tagtcacgaa cgtcttctgg ctcggctggg aaccacttgc
    11461 ggttgttaag cttgcaccag gcagcccaag aacggcacac tgagagaagc atcttccagg
    11521 tgtgctcgga gaaggcttgt ctgtcacgga acatatccat gaggttcttg cgaacttcat
    11581 cacttgtcgc gtcaactgga agtgctggaa gattttgatg aacagtgaga agatttgaca
    11641 tattttcgaa gttttttaga tgcactagaa caaagcgtgt tggcttcctc tgagcccgct
    11701 ttccttatat acccgcattc tgcagcctta cagaatgttc tagaaggtcc tagatgcatt
    11761 cgtttgaaaa tactcccggt gggtgcaaag agacgcagac ggaaaatgta tctgggtctc
    11821 tttattgtgt acactacttt tccatgtacc gaatgtgagt cgccctcctt ttgcaacaag
    11881 cagctcgaat gttctagaaa aaggtggaaa atagtataaa taccgttgaa aataaatacc
    11941 gaacaacatt tgctctaatt gtgaaattag aaatcttcaa actataatca tgtcacttta
    12001 ccactatttc cgtccagctc aacgttccgt ttttggtgat catttttgct ttcgtcgtaa
    12061 atctacacac gcgtctcttc cgtgcgagag tccaagccag cagccaaatt cgttgactga
    12121 gtattcaacg tttatacgtt gtcggcaacg agaaatagga aaatgcatcg ggaaatgttc
    12181 ttttttcgat tttttccaag gttttgacaa attttaccac gaattttgct atgttttcaa
    12241 ttaaaaaata tgttattcaa ctgtttctat gaggaaaata aggctttgca tgtaattttc
    12301 ttattcagca taatttttaa ttaatttgaa ttttctgtcc taacgtttat tttgttttct
    12361 tggttatgac tgatctgaaa ttaatttttg aattttaagg taatatgtca ggcggtgccg
    12421 caagtttgta caaaaaagca ggctccatga aaaagcctga actcaccgcg acgtctgtcg
    12481 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
    12541 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
    12601 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
    12661 tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct
    12721 cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
    12781 tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg
    12841 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
    12901 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
    12961 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
    13021 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
    13081 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
    13141 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
    13201 ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg
    13261 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc
    13321 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
    13381 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
    13441 gccccagcac tcgtccgagg gcaaaggaat agacccagct ttcttgtaca aagtgggtcc
    13501 aattactctt caacatccct acatgctctt tctccctgtg ctcccacccc ctatttttgt
    13561 tattatcaaa aaacttctct taatttcttt gttttttagc ttcttttaag tcacctctaa
    13621 caatgaaatt gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt
    13681 gtgctccctc cccccattaa taataattct atcccaaaat ctacacaatg ttctgtgtac
    13741 acttcttatg ttttttactt ctgataaatt tttttgaaac atcatagaaa aaaccgcaca
    13801 caaaatacct tatcatatgt tacgtttcag tttatgaccg caatttttaa taacttcgta
    13861 tagtatacct tatacgaagt tatactagta tatattttta tgttttcttt taaaccttcc
    13921 tccatatttc tgaattgaat gttgttgcat attgtgtgtc cttatttcat ttccatcatc
    13981 cttttgtgtg tttccccctt ccaccaattc cccccacgtc ctaatgaaat gaaagcgaga
    14041 agataaataa acttgcacat tgtgttcaga tgagaggagc ggaatacgaa ttgggagacg
    14101 gaaagagaaa aaaaaatgtt tagatttgga ttgaattaat tttttagaaa gcaagaaaaa
    14161 aaacacaaag agaataaata aataatagga aaaaaaacaa atattcagta tttaaaattt
    14221 gaaaattcaa cgacgttggc gtcgatcatc ctgtaaaacg acggccagtg ccaccgcgtt
    14281 ttgcttattg ctgtatttat gaaaaaaaaa acaatcaata ttggtcaagt aaataagaaa
    14341 aaattaacga atctctatct gacaccagat gcgaccctct attccacttc tctgttcatc
    14401 tgctgcttct tttgtttaac cagataaatc tccctcgggg aaaaccgtca aaaaaaggca
    14461 aactaaatgc aaacacgctc tatagacaaa atgtgtttgg tctcgtcacg aatggtgaga
    14521 gagaattggc ctccgccgca gagatcgctt gattattggc ctccagtggg caatgtcggg
    14581 gaaaaccaaa ctattgatga gaggtatcga cgaaaaatca acaatgacca actttttgtt
    14641 acagttttgt tataaatatg agttttggat attccattgc gtatttttct tttctacttt
    14701 caaaaaatct gctccaacct ttaatggctt ttcctgtctt gtcaaaatct ggatttttga
    14761 atatataatt tttaaaacca tcaaattcag cgaaatgaaa tcatgtaata caatttttta
    14821 ttttttccga ctgttgtgta ttccatcaaa ctattcaaaa aatcaatata atgatttttt
    14881 tttcattttt cgcgattttt tattattttg tcgtctgaaa acctttttac taataaaata
    14941 atttacaggg aaaaccacta acgactgtag cc
//
