LOCUS       sequence-32083-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Quillien A, Abdalla M, Yu J, Ou J, Zhu LJ, Lawson ND
  TITLE     Robust Identification of Developmentally Active Endothelial 
            Enhancers in Zebrafish Using FANS-Assisted ATAC-Seq.
  JOURNAL   Cell Rep. 2017 Jul 18;20(3):709-720. doi: 
            10.1016/j.celrep.2017.06.070.
  PUBMED    28723572
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.06"; journalName: "Cell Rep"; date: 
            "2017-07-18- 18"; volume: "20"; issue: "3"; pages: "709-720"
COMMENT     Sequence Label: 476 p5E-mcs
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    22..117
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        119..137
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    146..253
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     162..178
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     163..179
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(213..229)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(213..229)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(266..284)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    285..408
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(481..499)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(504..520)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(504..520)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(622..641)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(700..719)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gttacgctac catggagctc caaataatga ttttattttg actgatagtg acctgttcgt
       61 tgcaacaaat tgataagcaa tgctttttta taatgccaac tttgtataga aaagttggta
      121 atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggtcg acggtatcga
      181 taagcttgat atcgaattcc tgcagcccgg gggatccact agttctagag cggccgccac
      241 cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattcaagtt tgtacaaaaa
      301 agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
      361 aaaacagact acataatact gtaaaacaca acatatgcag tcactatgaa ccaactactt
      421 agatggtatt agtgacctgt agaattcgag ctctagagct gcagggcggc cgcgatatcc
      481 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
      541 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt
      601 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga
      661 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
      721 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt
      781 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
      841 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
      901 atgatgcatg gttactcacc actgcgatcc ccgg
//
