LOCUS       sequence-320833-        9229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of Flag-Polß(KTM: K41R/K61R/K81R) protein with 
            puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Fang Q, Inanc B, Schamus S, Wang XH, Wei L, Brown AR, Svilar D, 
            Sugrue KF, Goellner EM, Zeng X, Yates NA, Lan L, Vens C, Sobol RW
  TITLE     HSP90 regulates DNA repair via the interaction between XRCC1 and DNA
            polymerase beta.
  JOURNAL   Nat Commun. 2014 Nov 26;5:5513. doi: 10.1038/ncomms6513.
  PUBMED    25423885
REFERENCE   2  (bases 1 to 9229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-11-26"; volume: "5:5513. doi"; pages: " 
            10.1038/ncomms6513"
COMMENT     Sequence Label: pLVX-Flag-Polß(KTM)-IRES-puro
FEATURES             Location/Qualifiers
     source          1..9229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..234
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             419..463
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    726..841
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        899..1202
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1203..1406
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1357..1377
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1403..1427
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1522..1546
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1570..1593
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(2621..2645)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(2677..3251,3252..3254,3255..3263)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2844..2861)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3071..3090
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3283..3882
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3283..3302)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3779..3799
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3896..4484
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3949..3969)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4367..4378)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4691..5324
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5452..5468)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5452..5468)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5465..5487)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5476..5492
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5500..5506,5507..5524,5525..5530))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5545..5566
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5683..5700)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5854..6442)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5934..5953)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6613..7404,7405..7473))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7236..7255
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7474..7578)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7626..7760
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7663..7682)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7717..7736
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7928..8561
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8608..8733
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
       61 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
      121 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
      181 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
      241 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
      301 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
      361 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
      421 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
      481 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
      541 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
      601 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
      661 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
      721 tcgcctttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
      781 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
      841 ttcgggttta ttacagggac agcagagatc cagtttatcg ataagcttgg gagttccgcg
      901 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      961 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     1021 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     1081 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     1141 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     1201 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     1261 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     1321 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     1381 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc
     1441 atccacgctg ttttgacctc catagaagac accgactcta ctagaggatc tatttccggt
     1501 gaattcctcg agactagatc aacaagtttg tacaaaaaag caggctccgc ggccgccccc
     1561 ttcaccatgg actacaaaga cgatgacgat aaaggcatga gcaaacggaa ggcgccgcag
     1621 gagactctca acgggggaat caccgacatg ctcacagaac tcgcaaactt tgagaagaac
     1681 gtgagccaag ctatccacaa gtacaatgct tacagaagag cagcatctgt tatagcaaaa
     1741 tacccacaca aaataaagag tggagctgaa gctaagagat tgcctggagt aggaacaaaa
     1801 attgctgaaa agattgatga gtttttagca actggaagat tacgtaaact ggaaaagatt
     1861 cggcaggatg atacgagttc atccatcaat ttcctgactc gagttagtgg cattggtcca
     1921 tctgctgcaa ggaagtttgt agatgaagga attaaaacac tagaagatct cagaaaaaat
     1981 gaagataaat tgaaccatca tcagcgaatt gggctgaaat attttgggga ctttgaaaaa
     2041 agaattcctc gtgaagagat gttacaaatg caagatattg tactaaatga agttaaaaaa
     2101 gtggattctg aatacattgc tacagtctgt ggcagtttca gaagaggtgc agagtccagt
     2161 ggtgacatgg atgttctcct gacccatccc agcttcactt cagaatcaac caaacagcca
     2221 aaactgttac atcaggttgt ggagcagtta caaaaggttc attttatcac agataccctg
     2281 tcaaagggtg agacaaagtt catgggtgtt tgccagcttc ccagtaaaaa tgatgaaaaa
     2341 gaatatccac acagaagaat tgatatcagg ttgataccca aagatcagta ttactgtggt
     2401 gttctctatt tcactgggag tgatattttc aataagaata tgagggctca tgccctagaa
     2461 aagggtttca caatcaatga gtacaccatc cgtcccttgg gagtcactgg agttgcagga
     2521 gaacccctgc cagtggatag tgaaaaagac atctttgatt acatccagtg gaaataccgg
     2581 gaacccaagg accggagcga atgaaagggt gggcgcgccg acccagcttt cttgtacaaa
     2641 gtggttgatc tagttctaga gcggccgcgg atcccgcccc tctccctccc ccccccctaa
     2701 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     2761 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     2821 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     2881 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     2941 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     3001 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     3061 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     3121 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     3181 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     3241 acgatgataa gcttgccaca acccacaagg agacgacctt ccatgaccga gtacaagccc
     3301 acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg
     3361 ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc
     3421 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     3481 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     3541 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     3601 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     3661 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     3721 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     3781 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     3841 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct agacgcgtct ggaacaatca
     3901 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     3961 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     4021 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     4081 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     4141 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     4201 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     4261 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     4321 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     4381 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     4441 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc
     4501 agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     4561 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     4621 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     4681 aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
     4741 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
     4801 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
     4861 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
     4921 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
     4981 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
     5041 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
     5101 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
     5161 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     5221 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     5281 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     5341 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg
     5401 acattgctag cgtttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     5461 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     5521 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     5581 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     5641 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     5701 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     5761 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     5821 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5881 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5941 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6001 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6061 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6121 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6181 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6241 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     6301 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6361 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6421 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6481 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6541 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     6601 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     6661 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     6721 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     6781 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     6841 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     6901 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     6961 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7021 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7081 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7141 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     7201 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     7261 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     7321 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7381 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     7441 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     7501 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     7561 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     7621 agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     7681 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     7741 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
     7801 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg
     7861 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
     7921 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
     7981 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
     8041 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
     8101 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     8161 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     8221 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
     8281 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
     8341 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
     8401 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8461 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8521 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     8581 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     8641 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     8701 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     8761 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     8821 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     8881 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     8941 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     9001 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     9061 gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     9121 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     9181 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaat
//
