LOCUS       sequence-320834-       16728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SEC plasmid containing LG1 homology arms and 
            myo-3p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of 
            TIR1 and nuclear BFP reporter in C. elegans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16728)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 16728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pJH130
FEATURES             Location/Qualifiers
     source          1..16728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4360..4425
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             5281..5412
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             5413..5439
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     terminator      5448..5771
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(5804..5820)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5804..5820)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(7293..7313)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     rep_origin      complement(7450..8038)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7530..7549)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8209..9000,9001..9069))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8832..8851
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9070..9174)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9242..9260
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9298..9320)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    9643..9663
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(11170..11203)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(11203..11214)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(12636..12959)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    14877..14901
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             14904..15929
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             14904..15929
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(15930..15954)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(16307..16340)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     16709..16726
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     16710..16726
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ataaggctgc aacaaagatc aggttgacat atattttcag taatttattt taacctgtac
       61 tctatcactg ccggctataa taagttcttg aataaaataa ttttcccgac aaaacatgag
      121 tatttctttc gaaaataaaa gtgcaggcta attagagatt attctgtaat taactgcata
      181 atttgtcacg tgccatagtt ttacattcca ctacgtcata gttcttaaaa tactaatctc
      241 ctgaaaatag aagtaggtga agaaagttta attatcagtt ctaaaatgac aattgatctt
      301 tggaatatgt tctgaaacta ccgatcattg aacagatgct atttgaatga tatagaattg
      361 tatatttgca atttctgaaa cgcgttctta aaggcacaca gattaattca aaagggtctg
      421 gccgcaaaaa ggtttatggt ggccgatttt gagttttgtg tgtgattgct ttttcacaat
      481 cagtgttttc aggattatgt gatgaactag atcttcaagt ttcgttacat ttcatatgtt
      541 ttcggaactc acgaagtaca tattgggtat tgtgctcaaa aaattcagca atcagcttcg
      601 ctccgctgac tttagaaccc aaaaaaatag tatggccaaa ctgactgtgt tacgatcatt
      661 tcaatttttc aatacatatt taagatttct aagagtaaga aggtcaaaaa ctgttctgga
      721 atacatatat atttttcagg ttacaattag tcaaaaagtg cactgaaata tacgttttaa
      781 tttcacgaat aacccaatta gttcaatgta tttttggtca accaacgtta aagtttggct
      841 tccaaccaat tatcatttct gatcaaccac aatgtttttt ctttatctgc aagttaattt
      901 tttattttta tccagatgtt tggcatattt ttcaattctt cactagcgcc cacttcttgc
      961 acttccggcg ccctgaatct aatgcatctg ttgcaagaat tgaaagacca atcaacacat
     1021 tgttttcttc acgagatact gaagaaaatg aataaaaaca gagaaaaaga gccatgtgat
     1081 tagtgacaac tgttgctaac agataccata gcttggactt ggtacgtgat ggcaacgtat
     1141 gggtcaacaa aaatgattgc agagggggtg caaaacagtc aagtcgagaa aatatgaaaa
     1201 acagaaaaca aagaacagaa aaatgggttt gagagtcagt ataatttata aaagaaaaat
     1261 tgtacataga aattaaccat ttttgtagaa gaagttattt ttcaagcatc gttaaaaatt
     1321 attcaaagca ccttatttca tatttaattt taaacatggt taaatgaaca acacggtgcg
     1381 caatcaggaa aacttgaaat ctgaaactgt tgttgtgatc ttcttcgcaa ctgttcagat
     1441 agcactagtg taatgttaag agtgcgcgaa tataatggaa tataatggat cacacctcct
     1501 gccatcaggt aaacgtctct gttatcacat atttccaact attaaatttt taccttttac
     1561 agttttacat ttttttgaaa aaagtaactt tttgtcttca aaatccctga cgaaaatatc
     1621 aaatatttta atcgagactg cagaggaacc gattgatgat ttggaaaatc cagctttacc
     1681 tgtgtaagaa ctgaaaagtt tcataaccct agggtattcc cagttacatt ccccactggc
     1741 taacaatagc acccagtttt tcatcacctt tcttcaaatt tctcggcgat ttgttaaaaa
     1801 caaaatttgt gtcccttctc tgatatctct atgtctctaa acacaagttc atcggaaaac
     1861 gaaggagggt aggtgttggt tgggctcccg aagtgaaaat agaagagcaa gaatagaata
     1921 ttagagagag agtgcagaga gggcgggata gctcccggga ttccgttttc ttcttcttta
     1981 tcttcaacga tgatgtgtgt gcgtgttgta tagattctgt tgctccccca caactcgctc
     2041 cgaaggctca atacaattca attgatattg gaggagagcc taccggagtg ggaggataag
     2101 aagaaacata agaagaagaa gaagaagaag catgcttctg gtttttgatg ctatgaaaac
     2161 ggcacaaaaa gatgattgag gtcccttttc aataccttct ctcatctttc aaatcccatt
     2221 gaaacctaaa acttctcacc acgctttacc attgttctcc aaaaacttat agcaatgtct
     2281 ataacttttt tatctctgaa aagcagtgtt ccatttttct ttttcctatt ttatttcaat
     2341 tgtttctcac atttcgtttg gattctttgc ttgtcaacca gcttcttctt ccacttttac
     2401 cgtctaattt tcagggcagg gagccatcaa acccacgacc actagatcca tctagaaatg
     2461 caaaagagaa tcgccttgtc gtttccagaa gaagtactcg agcacgtctt ctccttcatc
     2521 caactcgaca aggaccgtaa ctccgtctcc ctcgtctgca agtcctggta cgagatcgag
     2581 cgttggtgcc gtcgtaaggt cttcatcgga aactgctacg ccgtctcccc agccaccgtc
     2641 atccgtcgtt tcccaaaggt ccgttccgtc gagctcaagg gaaagccaca cttcgccgac
     2701 ttcaacctcg tcccagacgg atggggagga tacgtctacc catggatcga ggccatgtcc
     2761 tcctcctaca cctggctcga ggagatccgt ctcaagcgta tggtcgtcac cgacgactgc
     2821 ctcgagctca tcgccaagtc cttcaagaac ttcaaggtcc tcgtcctctc ctcctgcgag
     2881 ggattctcca ccgacggact cgccgccatc gccgccacct gccgtaacct caaggtaagt
     2941 ttaaacatat atatactaac taaccctgat tatttaaatt ttcaggagct cgacctccgt
     3001 gagtccgacg tcgacgaggt ctccggacac tggctctccc acttcccaga cacctacacc
     3061 tccctcgtct ccctcaacat ctcctgcctc gcctccgagg tctccttctc cgccctcgag
     3121 cgtctcgtca cccgttgccc aaacctcaag tccctcaagc tcaaccgtgc cgtcccactc
     3181 gagaagctcg ccaccctcct ccaacgtgcc ccacaactcg aggagctcgg aaccggagga
     3241 tacaccgccg aggtccgtcc agacgtctac tccggactct ccgtcgccct ctccggatgc
     3301 aaggtaagtt taaacagttc ggtactaact aaccatacat atttaaattt tcaggagctc
     3361 cgttgcctct ccggattctg ggacgccgtc ccagcctacc tcccagccgt ctactccgtc
     3421 tgctcccgtc tcaccaccct caacctctcc tacgccaccg tccaatccta cgacctcgtc
     3481 aagctcctct gccaatgccc aaagctccaa cgtctctggg tcctcgacta catcgaggac
     3541 gccggactcg aggtcctcgc ctccacctgc aaggacctcc gtgagctccg tgtcttccca
     3601 tccgagccat tcgtcatgga gccaaacgtc gccctcaccg agcaaggact cgtctccgtc
     3661 tccatgggat gcccaaagct cgagtccgtc ctctacttct gccgtcaaat gaccaacgcc
     3721 gccctcatca ccatcgcccg taaccgtcca aacatgaccc gtttccgtct ctgcatcatc
     3781 gagccaaagg ccccagacta cctcaccctc gagccactcg acatcggatt cggagccatc
     3841 gtcgagcact gcaaggacct ccgtcgtctc tccctctccg gactcctcac cgacaaggtc
     3901 ttcgagtaca tcggaaccta cgccaagaag atggagatgc tctccgtcgc cttcgccgga
     3961 gactccgacc tcggactcca ccacgtcctc tccggatgcg actccctccg taagctcgag
     4021 atccgtgact gcccattcgg agacaaggcc ctcctcgcca acgcctccaa gctcgagacc
     4081 atgcgttccc tctggatgtc ctcctgctcc gtctccttcg gagcctgcaa gctcctcgga
     4141 caaaagatgc caaagctcaa cgtcgaggtc atcgacgagc gtggagcccc agactcccgt
     4201 ccagagtcct gcccagtcga gcgtgtcttc atctaccgta ccgtcgccgg accacgtttc
     4261 gacatgccag gattcgtctg gaacatggac caagactcca ccatgcgttt ctcccgtcaa
     4321 atcatcacca ccaacggact cggaggagga ggatccccag tcaagcaaac cctcaacttc
     4381 gacctcctca agctcgccgg agacgtcgag tccaacccag gaccaatggt ttcggaattg
     4441 attaaggaaa atatgcacat gaagctctac atggagggaa ccgtcgacaa ccaccacttc
     4501 aagtgcacct ccgagggaga gggaaagcca tacgagggaa cccaaaccat gcgtatcaag
     4561 gtcgtcgagg gaggaccact cccattcgcc ttcgacatcc tcgccacctc cttcctctac
     4621 ggatccaagg taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag
     4681 accttcatca accacaccca aggaatccca gacttcttca agcaatcctt cccagaggga
     4741 ttcacctggg agcgtgtcac cacctacgag gacggaggag tcctcaccgc cacccaagac
     4801 acctccctcc aagacggatg cctcatctac aacgtcaagg taagtttaaa cagttcggta
     4861 ctaactaacc atacatattt aaattttcag atccgtggag tcaacttcac ctccaacgga
     4921 ccagtcatgc aaaagaagac cctcggatgg gaggccttca ccgagaccct ctacccagcc
     4981 gacggaggac tcgagggacg taacgacatg gccctcaagc tcgtcggagg atcccacctc
     5041 atcgccaacg ccaaggtaag tttaaacatg attttactaa ctaactaatc tgatttaaat
     5101 tttcagacca cctaccgttc caagaagcca gccaagaacc tcaagatgcc aggagtctac
     5161 tacgtcgact accgtctcga gcgtatcaag gaggccaaca acgagaccta cgtcgagcaa
     5221 cacgaggtcg ccgtcgcccg ttactgcgac ctcccatcca agctcggaca caagctcaac
     5281 cctaaagatc cagccaaacc tccggccaag gcacaagttg tgggatggcc accggtgaga
     5341 tcataccgga agaacgtgat ggtttcctgc caaaaatcaa gcggtggccc ggaggcggcg
     5401 gcgttcgtga aaccagccgc caagcgtgtc aagctcgact aagataaatg caaaatcctt
     5461 tcaagcattc ccttcttctc tatcactctt ctttcttttt gtcaaaaaat tctctcgcta
     5521 atttatttgc ttttttaatg ttattatttt atgacttttt atagtcactg aaaagtttgc
     5581 atctgagtga agtgaatgct atcaaaatgt gattctgtct gatgtacttt cacaatctct
     5641 cttcaattcc attttgaagt gctttaaacc cgaaaggttg agaaaaatgc gagcgctcaa
     5701 atatttgtat tgtgttcgtt gagtgaccca acaaaaagag gaaactttat tgtgccgcca
     5761 agaaaaaagt ctcacgtacg gtgcgcgcga tgccctaggc atggtcatag ctgtttcctg
     5821 cgcaagtcgg cgatttcttt gaagttttgt caaaagaaga gacaaggact tgatagatac
     5881 gctcatcaga gtgagattca ttgcaaatag tgtgtaaatc gtggagagac gagagaaaag
     5941 gtgtgagaag cttagaagat aaagaatagg aagaagaaca tatctcattt cctattttct
     6001 ctaatttttc gttcattttt ctagacatta cccatttgat ggtttttcaa tatggttatc
     6061 agggtactca ttttggtagt tttcaaagca atttggagtt tttttttaaa ttgtcaacta
     6121 caaaaacgca gcttaaaggt ccaaatatca tagtataaaa attctttaaa aaattccaaa
     6181 ttttatatga ttttttgaaa attggaagaa tgttagtttt tgccattttt ttggaagttt
     6241 ttctccggat tttatggtac ttatatttca aatataaatt tttgtaggaa ttcgagtatg
     6301 cgagagagct atagtttact aaaaaaaagt ggcaaaaact cagtaattgc cacttttttc
     6361 aaaaatgttt tgaaaaattt tactatgata tttggccatt ttggcaccat atgtacgtta
     6421 ctttttacga aacactctaa tttttgaaat ttgagtttca tgtttaatct tttgtataaa
     6481 atttttatgg tccttttgtg acaggataaa aaattgcaaa aaaatagaaa actttgtttc
     6541 gtgaaaacac ctagtttaaa aattgtgggc cacttagcga ttctagtcaa aaatcagaca
     6601 aatgtcagct gccgtaactt tttccggatc agggttatca aattttaatt ttaattgtaa
     6661 atgtagaaaa atgatcggca acattttgct aattgaaaat attttgaaaa ttgctatagt
     6721 acatattaat ctagtattaa tatagtatta atgttataat atcagttatg tgtttgaata
     6781 actcaagcgt aagtttccgt aaaatatctt taaattttgg taatttttta cggtttggta
     6841 taaatttttg cattttttga ttttctacac tttttttcga tttcgcattt tccgaatttc
     6901 tctgaaataa atcgaacaat cggaaaattt tcgagggcac ccttagccat gcagctcagg
     6961 aatttgactt tatgttccca ttcaaatttt tttaaaatta aataaaatgc taattgtaag
     7021 ataaattaca aaaattatta aatctcatca tccttatgat gatagtgcag tgcatgaata
     7081 tatttttcca aataggatat gattcgtcga gtagcttcac gtaatccgtt ttcttcaatc
     7141 accatttcga actgcaattt tttttttgga tttttgtaac aatattgtca taaattgtga
     7201 ttttgatatt ttgcgtagag catcagaacc tttctgcttt gaattttaat gggattaaaa
     7261 attataaaaa acttgcaccc taattaaagc gccaacttta ttatacatag ttgataattc
     7321 gatggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7381 aggaaagaac atgtgagcaa aaggccagca aaagaccagg aaccgtaaaa aggccgcgtt
     7441 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7501 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7561 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7621 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7681 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7741 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7801 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7861 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7921 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7981 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8041 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8101 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8161 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8221 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8281 ccccgtcgtg tagataacta cgatacggga gggcttacca tctgacccca gtgctgcaat
     8341 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8401 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8461 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8521 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8581 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8641 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8701 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8761 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8821 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8881 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8941 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9001 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9061 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9121 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9181 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     9241 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     9301 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     9361 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     9421 ggcatcagag cagattgcac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     9481 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     9541 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     9601 aaggcgatta agttgggtaa cgccagggat ccgccaagct atcaactttg tatagaaaag
     9661 ttgcaagtgg ggatcaggaa gaagttgata aagtgattca aaaactcgag gagctcaaca
     9721 cagcgatgaa cccaattttg agagcaacac tgacgaagaa ctatgacgcg agggtaggca
     9781 ctaccttgaa gctatccacc tgcctgcctg ccgcctattt ccatgtgcct actgcgcatg
     9841 gaaataggcg gtagtcaggc aggtagatag cctcataaaa ttcaatactt ccttttcaga
     9901 aagaatgctg gaatgtattc aaagaggagt cactcttccc attgctcaga gaatatgaag
     9961 agttgctcgg cgagcaaaag ttcatgattg gagatcgata ttcatgggct gacatcgctg
    10021 tcgtcgagtt tctaactcgg tgtcagcagt gctacgatag cttctatctg gctcatttcg
    10081 agaggctccg aactttttgc cagacttttg agtctcttcc gcacattcgt ccctacattc
    10141 agggacgtgt tgattcattt atctaatttt ttaaaatgga tatttttcgt tttttatttg
    10201 tttgttttgc tgataattca ataaagttac gcaacgaatt ttagacattt atgaaaaccg
    10261 aaaatcgaca aaaatatttt aagaaaaata gcagcaaaat tcaatatttt atcaaatttt
    10321 gtttatggta atgagaaaga aaacatacaa tatttttcta agaagaagaa aaatgaccag
    10381 aattttaaga tttttctcga atatacgaaa aattgataaa ctaaaatttt cgatttttcc
    10441 atttattttg aaaaaaaaac tcttgacggt tttcaccatg cgaagaggtg agaaatattc
    10501 tgaaagttat gcattttgaa aaaattgcaa aaaaatacgt taaaattttt tgatctattg
    10561 tttttttttg aaaaccggaa tttaaaatgg aatttttttt caaaaattaa aattaaaatt
    10621 taaatatttc tagtgtttgg atagtttttc aaaagctaga tttaacccgc gagatgtcga
    10681 ccgctagtgt agcttacaga tggctgaaga cttcgcaaaa atcttatctt agaaacccgc
    10741 gaggtgtcgg ccgctaaaac tttgtaaacc tgccaacaaa atgcttcgca ttgtctgaac
    10801 agatgaaacc ttgaaacaaa acatatacaa ttgaaatccc gtaaagtgtc gggcgcaaga
    10861 actgcgatta gttgtataat tggactagct caaatttgac caaaatctca tttttagacc
    10921 tctaagatgt cggccactat aactttattc aacttgaaac tgcaaaaaaa cttatcaact
    10981 gtatagattc aagtattatg tcaagtattg gttttagagc tacagtacac tctgataaca
    11041 aaatctgcag aaatacctcc ctgtcaattc ccaaaatacc tcccctcatc tcaattatcc
    11101 cgaaaatcgg ttagtcattg ccatcagaaa atcgaattag gtaccatgat ggtagcaaac
    11161 tcacttcgta taacttcgta tagtatacct tatacgaagt tatctgccct cgattgtcta
    11221 tcttccattc tcaccaagtt tcaacaactt tattcagtca ttcttctctg gttcatttcc
    11281 caaccaatct tcccgaataa agcccatctg tcctttcggc cacattcctc acctacatcc
    11341 gtactcctta tctcccgttc ttatccagtc ttttatcgtc tctgcgtctc gcacactgtc
    11401 tcgtcggtaa tgtaaaaaaa aaacaaaaaa aacttgaaaa cttgcggaaa gatttcctaa
    11461 taaaaggaga cgaatccggt aggaaatcga caagtctctg cttctcgaag actaggtgag
    11521 ttacatttct ttggccgaat tattttgaaa tcctaaattt gataatttca gagatgtctg
    11581 taaaacttgc gtgttatgtg acggcttcgg tcactgtcgc cactcttatg gtatgcttca
    11641 tgaccatgtc aaccatctac tcggaggttg atggattcag agagaagctt gataccgaga
    11701 tgaatgtgtt cagagtgagt taaattatat tttgaatttt aaataatttt taattttcta
    11761 gcaatctacc aatggattgt ggaaggacat agttgtcatc ggaagatcta gcaagcgtgt
    11821 ccgttgtcaa tatgaagaga ccaacgctac cccaactcca catgctgatg gatccccatc
    11881 tgctccacca ggtcaaccac cagcagttcc accagtcttc aaccagccaa agactccaaa
    11941 tggagccaat ggaaatggac caacctgcaa ctgcaatgct gataacaagt gcccagctgg
    12001 accatccgga ccaaagggag ttccaggagt tccaggactc gacggagttc caggacttga
    12061 cggtgttcca ggagttggag ctgatgatat cgctccacaa cgcgagtctg tcggatgctt
    12121 cacttgccca caaggaccag ttggaccacc aggagctctt ggaagaccag gaccacgtgg
    12181 acttccagga ccaagaggac aaaatggaaa cccaggaaga gatggacaac caggacatcc
    12241 aggagagcaa ggatcatccg gtcaaatcgg aaagatcgga gagccaggac caccaggaga
    12301 gaagggacgc gacgccgagc atccaatcgg aagaccagga ccaaagggac caagaggaga
    12361 tcaaggacca acaggaccag ctggacagaa cggtcttcac ggaccaccag gagagccagg
    12421 aaccgttgga ccagaaggac catctggaaa gcaaggacgt caaggaccag acggaaccca
    12481 gggagagact ggaccagacg gaagaccagg aaaggatgcc gagtactgcc agtgcccaga
    12541 caagtctcca ccatcagagg ctgtcaacgc caaccgtgga tacagaaata tctaaattgt
    12601 tggtgttttc taataaaata tttgagattc agtgagactt ttttcttggc ggcacaataa
    12661 agtttcctct ttttgttggg tcactcaacg aacacaatac aaatatttga gcgctcgcat
    12721 ttttctcaac ctttcgggtt taaagcactt caaaatggaa ttgaagagag attgtgaaag
    12781 tacatcagac agaatcacat tttgatagca ttcacttcac tcagatgcaa acttttcagt
    12841 gactataaaa agtcataaaa taataacatt aaaaaagcaa ataaattagc gagagaattt
    12901 tttgacaaaa agaaagaata gtgatagaga agaagggaat gcttgaaagg attttgcatt
    12961 tatcttaatc accgtcttcg agaagacgaa ccatggcgcc agtttcagaa tccaagtttc
    13021 taatatagtt catcacaatg ttgacgttgg tccaacctcc ggcttgcata atctctggga
    13081 tggaaactcc agcgcgagcc atgtcacggg cagctccgac acgggcggaa tgtccagacc
    13141 aagccaagta acgttgtccg gaatcatcct tggctccgta gatgagacgg tgggtagctt
    13201 cgaagattcc tgaaaattta ttttcagctt attcttagct aatatgttgt ttaaacttac
    13261 cttcaagagc tcgagtcgag agttgtgatg ttgcactcgg ggcagcaact ccgttcttgc
    13321 gaacacgaca gaacaggtag ttgtttggat catcggcgac tccggaaacc gaaatccagc
    13381 gctcaacgag cttggtaact ccaagggaga gcgctttttc gactccggca gtcgagacca
    13441 gcgtttttgt acgtccgatg tggatgagca ttcttcctcc gtctgtgcga ctaatatcct
    13501 tgacgcgaat gcgggcaatt tcggcgatac ggagaagtgt gttgtaagca attccgagga
    13561 aggcgaggtt tcggatgtcc tggcaacgat cggaattttc cataagagaa cggacctgat
    13621 cgaagtcggt tctgaaaatt tcgtcaagtc ttaggattga atacattaat atttaaactt
    13681 acctttcgaa ggcgagggct tgcttggctc tctctccagc atcaacattt tccttgcgga
    13741 tacgtctcat tacgagagat acggcattag aatctgatgg gcgtggaagt ccagatcggc
    13801 gatggagcat attgagctgt ccgagatgtt gttggatcgt cttcaccgcc aatccacgag
    13861 cttggaggta gagcaagtag tcacgaacgt cttctggctc ggctgggaac cacttgcggt
    13921 tgttaagctt gcaccaggca gcccaagaac ggcacactga gagaagcatc ttccaggtgt
    13981 gctcggagaa ggcttgtctg tcacggaaca tatccatgag gttcttgcga acttcatcac
    14041 ttgtcgcgtc aactggaagt gctggaagat tttgatgaac agtgagaaga tttgacatat
    14101 tttcgaagtt ttttagatgc actagaacaa agcgtgttgg cttcctctga gcccgctttc
    14161 cttatatacc cgcattctgc agccttacag aatgttctag aaggtcctag atgcattcgt
    14221 ttgaaaatac tcccggtggg tgcaaagaga cgcagacgga aaatgtatct gggtctcttt
    14281 attgtgtaca ctacttttcc atgtaccgaa tgtgagtcgc cctccttttg caacaagcag
    14341 ctcgaatgtt ctagaaaaag gtggaaaata gtataaatac cgttgaaaat aaataccgaa
    14401 caacatttgc tctaattgtg aaattagaaa tcttcaaact ataatcatgt cactttacca
    14461 ctatttccgt ccagctcaac gttccgtttt tggtgatcat ttttgctttc gtcgtaaatc
    14521 tacacacgcg tctcttccgt gcgagagtcc aagccagcag ccaaattcgt tgactgagta
    14581 ttcaacgttt atacgttgtc ggcaacgaga aataggaaaa tgcatcggga aatgttcttt
    14641 tttcgatttt ttccaaggtt ttgacaaatt ttaccacgaa ttttgctatg ttttcaatta
    14701 aaaaatatgt tattcaactg tttctatgag gaaaataagg ctttgcatgt aattttctta
    14761 ttcagcataa tttttaatta atttgaattt tctgtcctaa cgtttatttt gttttcttgg
    14821 ttatgactga tctgaaatta atttttgaat tttaaggtaa tatgtcaggc ggtgccgcaa
    14881 gtttgtacaa aaaagcaggc tccatgaaaa agcctgaact caccgcgacg tctgtcgaga
    14941 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
    15001 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
    15061 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
    15121 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
    15181 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
    15241 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
    15301 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
    15361 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
    15421 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
    15481 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
    15541 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
    15601 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
    15661 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
    15721 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
    15781 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
    15841 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
    15901 ccagcactcg tccgagggca aaggaataga cccagctttc ttgtacaaag tgggtccaat
    15961 tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta tttttgttat
    16021 tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc ttttaagtca cctctaacaa
    16081 tgaaattgtg tagattcaaa aatagaatta attcgtaata aaaagtcgaa aaaaattgtg
    16141 ctccctcccc ccattaataa taattctatc ccaaaatcta cacaatgttc tgtgtacact
    16201 tcttatgttt tttacttctg ataaattttt ttgaaacatc atagaaaaaa ccgcacacaa
    16261 aataccttat catatgttac gtttcagttt atgaccgcaa tttttaataa cttcgtatag
    16321 tataccttat acgaagttat actagtatat atttttatgt tttcttttaa accttcctcc
    16381 atatttctga attgaatgtt gttgcatatt gtgtgtcctt atttcatttc catcatcctt
    16441 ttgtgtgttt cccccttcca ccaattcccc ccacgtccta atgaaatgaa agcgagaaga
    16501 taaataaact tgcacattgt gttcagatga gaggagcgga atacgaattg ggagacggaa
    16561 agagaaaaaa aaatgtttag atttggattg aattaatttt ttagaaagca agaaaaaaaa
    16621 cacaaagaga ataaataaat aataggaaaa aaaacaaata ttcagtattt aaaatttgaa
    16681 aattcaacga cgttggcgtc gatcatcctg taaaacgacg gccagtgc
//
