LOCUS       sequence-320838-       15233 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SEC plasmid containing LG1 homology arms and 
            myo-2p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of 
            TIR1 and nuclear BFP reporter in C. elegans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15233)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 15233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pJH127
FEATURES             Location/Qualifiers
     source          1..15233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2865..2930
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             3786..3917
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             3918..3944
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     terminator      3953..4276
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(4309..4325)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4309..4325)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(5798..5818)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     rep_origin      complement(5955..6543)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6035..6054)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6714..7505,7506..7574))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7337..7356
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7575..7679)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7747..7765
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7803..7825)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    8148..8168
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(9675..9708)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(9708..9719)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(11141..11464)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    13382..13406
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             13409..14434
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             13409..14434
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(14435..14459)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(14812..14845)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     15214..15231
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15215..15231
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 attttatatc tgagtagtat cctttgcttt aaatgtccat aaaactaatt ttataatcaa
       61 taaaacaacg tttgtaaatc aactgagttt acaagtagag acattgaggg atactttcac
      121 tatgctaaag tgataatcga ccaaataata acttcacttt ggtatttatt cctgtcttat
      181 aaatgttatg tatgaattaa attcatatgc atatggctca ctctgacaat ttttaataat
      241 cttccagatc aatattgact accgatgcgg gtggtctttt gctttgaatt ctgctgaact
      301 ttacaccccg aacagcaatg tgtgcttcag cctaaaaaaa agtaagtgtg ttaatcagtg
      361 cccccgattc ttcatttttt gcccctctct cccgtttcgt cggcaaaaga agagaaaata
      421 aagataagtc tcaagatagg ttggtaatcg ctaaagtggt tgtgtggata agagtagcaa
      481 aatggcagga agagcacttt gcgcgcacac actgtactca ttgttctgga taaaattctc
      541 tcgttgtttg ccgtcggatg tctgcctctc tgcattgagc cggcttcttc actatcttta
      601 gttaacctaa aatgccgttt cttttctcgt atccccacta tcccgttgag gttctctgct
      661 ctcttcgctc cctaccgcca gcgagcaact atccgtgggg gcgccttgct cggaagatgg
      721 gggggaagaa agaagatttt tgctatttgc acttgagaaa gagacttttc ctgcgtcgat
      781 ggtcagagaa cagtgtgcag acacttttca gctacctaga attacaattg gatatccccg
      841 cctcccaatc cacccaccca gggaaaaaga agggctcgcc gaaaatcaaa gttatctcca
      901 ggctcgcgca tcccaccgag cggttgactt ctctccacca cttttcattt ttaccctcga
      961 ccatgcaaaa gagaatcgcc ttgtcgtttc cagaagaagt actcgagcac gtcttctcct
     1021 tcatccaact cgacaaggac cgtaactccg tctccctcgt ctgcaagtcc tggtacgaga
     1081 tcgagcgttg gtgccgtcgt aaggtcttca tcggaaactg ctacgccgtc tccccagcca
     1141 ccgtcatccg tcgtttccca aaggtccgtt ccgtcgagct caagggaaag ccacacttcg
     1201 ccgacttcaa cctcgtccca gacggatggg gaggatacgt ctacccatgg atcgaggcca
     1261 tgtcctcctc ctacacctgg ctcgaggaga tccgtctcaa gcgtatggtc gtcaccgacg
     1321 actgcctcga gctcatcgcc aagtccttca agaacttcaa ggtcctcgtc ctctcctcct
     1381 gcgagggatt ctccaccgac ggactcgccg ccatcgccgc cacctgccgt aacctcaagg
     1441 taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag gagctcgacc
     1501 tccgtgagtc cgacgtcgac gaggtctccg gacactggct ctcccacttc ccagacacct
     1561 acacctccct cgtctccctc aacatctcct gcctcgcctc cgaggtctcc ttctccgccc
     1621 tcgagcgtct cgtcacccgt tgcccaaacc tcaagtccct caagctcaac cgtgccgtcc
     1681 cactcgagaa gctcgccacc ctcctccaac gtgccccaca actcgaggag ctcggaaccg
     1741 gaggatacac cgccgaggtc cgtccagacg tctactccgg actctccgtc gccctctccg
     1801 gatgcaaggt aagtttaaac agttcggtac taactaacca tacatattta aattttcagg
     1861 agctccgttg cctctccgga ttctgggacg ccgtcccagc ctacctccca gccgtctact
     1921 ccgtctgctc ccgtctcacc accctcaacc tctcctacgc caccgtccaa tcctacgacc
     1981 tcgtcaagct cctctgccaa tgcccaaagc tccaacgtct ctgggtcctc gactacatcg
     2041 aggacgccgg actcgaggtc ctcgcctcca cctgcaagga cctccgtgag ctccgtgtct
     2101 tcccatccga gccattcgtc atggagccaa acgtcgccct caccgagcaa ggactcgtct
     2161 ccgtctccat gggatgccca aagctcgagt ccgtcctcta cttctgccgt caaatgacca
     2221 acgccgccct catcaccatc gcccgtaacc gtccaaacat gacccgtttc cgtctctgca
     2281 tcatcgagcc aaaggcccca gactacctca ccctcgagcc actcgacatc ggattcggag
     2341 ccatcgtcga gcactgcaag gacctccgtc gtctctccct ctccggactc ctcaccgaca
     2401 aggtcttcga gtacatcgga acctacgcca agaagatgga gatgctctcc gtcgccttcg
     2461 ccggagactc cgacctcgga ctccaccacg tcctctccgg atgcgactcc ctccgtaagc
     2521 tcgagatccg tgactgccca ttcggagaca aggccctcct cgccaacgcc tccaagctcg
     2581 agaccatgcg ttccctctgg atgtcctcct gctccgtctc cttcggagcc tgcaagctcc
     2641 tcggacaaaa gatgccaaag ctcaacgtcg aggtcatcga cgagcgtgga gccccagact
     2701 cccgtccaga gtcctgccca gtcgagcgtg tcttcatcta ccgtaccgtc gccggaccac
     2761 gtttcgacat gccaggattc gtctggaaca tggaccaaga ctccaccatg cgtttctccc
     2821 gtcaaatcat caccaccaac ggactcggag gaggaggatc cccagtcaag caaaccctca
     2881 acttcgacct cctcaagctc gccggagacg tcgagtccaa cccaggacca atggtttcgg
     2941 aattgattaa ggaaaatatg cacatgaagc tctacatgga gggaaccgtc gacaaccacc
     3001 acttcaagtg cacctccgag ggagagggaa agccatacga gggaacccaa accatgcgta
     3061 tcaaggtcgt cgagggagga ccactcccat tcgccttcga catcctcgcc acctccttcc
     3121 tctacggatc caaggtaagt ttaaacatat atatactaac taaccctgat tatttaaatt
     3181 ttcagacctt catcaaccac acccaaggaa tcccagactt cttcaagcaa tccttcccag
     3241 agggattcac ctgggagcgt gtcaccacct acgaggacgg aggagtcctc accgccaccc
     3301 aagacacctc cctccaagac ggatgcctca tctacaacgt caaggtaagt ttaaacagtt
     3361 cggtactaac taaccataca tatttaaatt ttcagatccg tggagtcaac ttcacctcca
     3421 acggaccagt catgcaaaag aagaccctcg gatgggaggc cttcaccgag accctctacc
     3481 cagccgacgg aggactcgag ggacgtaacg acatggccct caagctcgtc ggaggatccc
     3541 acctcatcgc caacgccaag gtaagtttaa acatgatttt actaactaac taatctgatt
     3601 taaattttca gaccacctac cgttccaaga agccagccaa gaacctcaag atgccaggag
     3661 tctactacgt cgactaccgt ctcgagcgta tcaaggaggc caacaacgag acctacgtcg
     3721 agcaacacga ggtcgccgtc gcccgttact gcgacctccc atccaagctc ggacacaagc
     3781 tcaaccctaa agatccagcc aaacctccgg ccaaggcaca agttgtggga tggccaccgg
     3841 tgagatcata ccggaagaac gtgatggttt cctgccaaaa atcaagcggt ggcccggagg
     3901 cggcggcgtt cgtgaaacca gccgccaagc gtgtcaagct cgactaagat aaatgcaaaa
     3961 tcctttcaag cattcccttc ttctctatca ctcttctttc tttttgtcaa aaaattctct
     4021 cgctaattta tttgcttttt taatgttatt attttatgac tttttatagt cactgaaaag
     4081 tttgcatctg agtgaagtga atgctatcaa aatgtgattc tgtctgatgt actttcacaa
     4141 tctctcttca attccatttt gaagtgcttt aaacccgaaa ggttgagaaa aatgcgagcg
     4201 ctcaaatatt tgtattgtgt tcgttgagtg acccaacaaa aagaggaaac tttattgtgc
     4261 cgccaagaaa aaagtctcac gtacggtgcg cgcgatgccc taggcatggt catagctgtt
     4321 tcctgcgcaa gtcggcgatt tctttgaagt tttgtcaaaa gaagagacaa ggacttgata
     4381 gatacgctca tcagagtgag attcattgca aatagtgtgt aaatcgtgga gagacgagag
     4441 aaaaggtgtg agaagcttag aagataaaga ataggaagaa gaacatatct catttcctat
     4501 tttctctaat ttttcgttca tttttctaga cattacccat ttgatggttt ttcaatatgg
     4561 ttatcagggt actcattttg gtagttttca aagcaatttg gagttttttt ttaaattgtc
     4621 aactacaaaa acgcagctta aaggtccaaa tatcatagta taaaaattct ttaaaaaatt
     4681 ccaaatttta tatgattttt tgaaaattgg aagaatgtta gtttttgcca tttttttgga
     4741 agtttttctc cggattttat ggtacttata tttcaaatat aaatttttgt aggaattcga
     4801 gtatgcgaga gagctatagt ttactaaaaa aaagtggcaa aaactcagta attgccactt
     4861 ttttcaaaaa tgttttgaaa aattttacta tgatatttgg ccattttggc accatatgta
     4921 cgttactttt tacgaaacac tctaattttt gaaatttgag tttcatgttt aatcttttgt
     4981 ataaaatttt tatggtcctt ttgtgacagg ataaaaaatt gcaaaaaaat agaaaacttt
     5041 gtttcgtgaa aacacctagt ttaaaaattg tgggccactt agcgattcta gtcaaaaatc
     5101 agacaaatgt cagctgccgt aactttttcc ggatcagggt tatcaaattt taattttaat
     5161 tgtaaatgta gaaaaatgat cggcaacatt ttgctaattg aaaatatttt gaaaattgct
     5221 atagtacata ttaatctagt attaatatag tattaatgtt ataatatcag ttatgtgttt
     5281 gaataactca agcgtaagtt tccgtaaaat atctttaaat tttggtaatt ttttacggtt
     5341 tggtataaat ttttgcattt tttgattttc tacacttttt ttcgatttcg cattttccga
     5401 atttctctga aataaatcga acaatcggaa aattttcgag ggcaccctta gccatgcagc
     5461 tcaggaattt gactttatgt tcccattcaa atttttttaa aattaaataa aatgctaatt
     5521 gtaagataaa ttacaaaaat tattaaatct catcatcctt atgatgatag tgcagtgcat
     5581 gaatatattt ttccaaatag gatatgattc gtcgagtagc ttcacgtaat ccgttttctt
     5641 caatcaccat ttcgaactgc aatttttttt ttggattttt gtaacaatat tgtcataaat
     5701 tgtgattttg atattttgcg tagagcatca gaacctttct gctttgaatt ttaatgggat
     5761 taaaaattat aaaaaacttg caccctaatt aaagcgccaa ctttattata catagttgat
     5821 aattcgatgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5881 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaaga ccaggaaccg taaaaaggcc
     5941 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6001 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6061 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6121 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6181 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6241 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6301 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6361 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     6421 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6481 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6541 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6601 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6661 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     6721 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     6781 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctga ccccagtgct
     6841 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     6901 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     6961 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7021 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7081 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7141 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7201 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7261 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7321 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7381 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7441 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7501 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7561 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7621 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7681 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     7741 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     7801 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     7861 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     7921 tatgcggcat cagagcagat tgcactgaga gtgcaccata tgcggtgtga aataccgcac
     7981 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
     8041 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     8101 gctgcaaggc gattaagttg ggtaacgcca gggatccgcc aagctatcaa ctttgtatag
     8161 aaaagttgca agtggggatc aggaagaagt tgataaagtg attcaaaaac tcgaggagct
     8221 caacacagcg atgaacccaa ttttgagagc aacactgacg aagaactatg acgcgagggt
     8281 aggcactacc ttgaagctat ccacctgcct gcctgccgcc tatttccatg tgcctactgc
     8341 gcatggaaat aggcggtagt caggcaggta gatagcctca taaaattcaa tacttccttt
     8401 tcagaaagaa tgctggaatg tattcaaaga ggagtcactc ttcccattgc tcagagaata
     8461 tgaagagttg ctcggcgagc aaaagttcat gattggagat cgatattcat gggctgacat
     8521 cgctgtcgtc gagtttctaa ctcggtgtca gcagtgctac gatagcttct atctggctca
     8581 tttcgagagg ctccgaactt tttgccagac ttttgagtct cttccgcaca ttcgtcccta
     8641 cattcaggga cgtgttgatt catttatcta attttttaaa atggatattt ttcgtttttt
     8701 atttgtttgt tttgctgata attcaataaa gttacgcaac gaattttaga catttatgaa
     8761 aaccgaaaat cgacaaaaat attttaagaa aaatagcagc aaaattcaat attttatcaa
     8821 attttgttta tggtaatgag aaagaaaaca tacaatattt ttctaagaag aagaaaaatg
     8881 accagaattt taagattttt ctcgaatata cgaaaaattg ataaactaaa attttcgatt
     8941 tttccattta ttttgaaaaa aaaactcttg acggttttca ccatgcgaag aggtgagaaa
     9001 tattctgaaa gttatgcatt ttgaaaaaat tgcaaaaaaa tacgttaaaa ttttttgatc
     9061 tattgttttt ttttgaaaac cggaatttaa aatggaattt tttttcaaaa attaaaatta
     9121 aaatttaaat atttctagtg tttggatagt ttttcaaaag ctagatttaa cccgcgagat
     9181 gtcgaccgct agtgtagctt acagatggct gaagacttcg caaaaatctt atcttagaaa
     9241 cccgcgaggt gtcggccgct aaaactttgt aaacctgcca acaaaatgct tcgcattgtc
     9301 tgaacagatg aaaccttgaa acaaaacata tacaattgaa atcccgtaaa gtgtcgggcg
     9361 caagaactgc gattagttgt ataattggac tagctcaaat ttgaccaaaa tctcattttt
     9421 agacctctaa gatgtcggcc actataactt tattcaactt gaaactgcaa aaaaacttat
     9481 caactgtata gattcaagta ttatgtcaag tattggtttt agagctacag tacactctga
     9541 taacaaaatc tgcagaaata cctccctgtc aattcccaaa atacctcccc tcatctcaat
     9601 tatcccgaaa atcggttagt cattgccatc agaaaatcga attaggtacc atgatggtag
     9661 caaactcact tcgtataact tcgtatagta taccttatac gaagttatct gccctcgatt
     9721 gtctatcttc cattctcacc aagtttcaac aactttattc agtcattctt ctctggttca
     9781 tttcccaacc aatcttcccg aataaagccc atctgtcctt tcggccacat tcctcaccta
     9841 catccgtact ccttatctcc cgttcttatc cagtctttta tcgtctctgc gtctcgcaca
     9901 ctgtctcgtc ggtaatgtaa aaaaaaaaca aaaaaaactt gaaaacttgc ggaaagattt
     9961 cctaataaaa ggagacgaat ccggtaggaa atcgacaagt ctctgcttct cgaagactag
    10021 gtgagttaca tttctttggc cgaattattt tgaaatccta aatttgataa tttcagagat
    10081 gtctgtaaaa cttgcgtgtt atgtgacggc ttcggtcact gtcgccactc ttatggtatg
    10141 cttcatgacc atgtcaacca tctactcgga ggttgatgga ttcagagaga agcttgatac
    10201 cgagatgaat gtgttcagag tgagttaaat tatattttga attttaaata atttttaatt
    10261 ttctagcaat ctaccaatgg attgtggaag gacatagttg tcatcggaag atctagcaag
    10321 cgtgtccgtt gtcaatatga agagaccaac gctaccccaa ctccacatgc tgatggatcc
    10381 ccatctgctc caccaggtca accaccagca gttccaccag tcttcaacca gccaaagact
    10441 ccaaatggag ccaatggaaa tggaccaacc tgcaactgca atgctgataa caagtgccca
    10501 gctggaccat ccggaccaaa gggagttcca ggagttccag gactcgacgg agttccagga
    10561 cttgacggtg ttccaggagt tggagctgat gatatcgctc cacaacgcga gtctgtcgga
    10621 tgcttcactt gcccacaagg accagttgga ccaccaggag ctcttggaag accaggacca
    10681 cgtggacttc caggaccaag aggacaaaat ggaaacccag gaagagatgg acaaccagga
    10741 catccaggag agcaaggatc atccggtcaa atcggaaaga tcggagagcc aggaccacca
    10801 ggagagaagg gacgcgacgc cgagcatcca atcggaagac caggaccaaa gggaccaaga
    10861 ggagatcaag gaccaacagg accagctgga cagaacggtc ttcacggacc accaggagag
    10921 ccaggaaccg ttggaccaga aggaccatct ggaaagcaag gacgtcaagg accagacgga
    10981 acccagggag agactggacc agacggaaga ccaggaaagg atgccgagta ctgccagtgc
    11041 ccagacaagt ctccaccatc agaggctgtc aacgccaacc gtggatacag aaatatctaa
    11101 attgttggtg ttttctaata aaatatttga gattcagtga gacttttttc ttggcggcac
    11161 aataaagttt cctctttttg ttgggtcact caacgaacac aatacaaata tttgagcgct
    11221 cgcatttttc tcaacctttc gggtttaaag cacttcaaaa tggaattgaa gagagattgt
    11281 gaaagtacat cagacagaat cacattttga tagcattcac ttcactcaga tgcaaacttt
    11341 tcagtgacta taaaaagtca taaaataata acattaaaaa agcaaataaa ttagcgagag
    11401 aattttttga caaaaagaaa gaatagtgat agagaagaag ggaatgcttg aaaggatttt
    11461 gcatttatct taatcaccgt cttcgagaag acgaaccatg gcgccagttt cagaatccaa
    11521 gtttctaata tagttcatca caatgttgac gttggtccaa cctccggctt gcataatctc
    11581 tgggatggaa actccagcgc gagccatgtc acgggcagct ccgacacggg cggaatgtcc
    11641 agaccaagcc aagtaacgtt gtccggaatc atccttggct ccgtagatga gacggtgggt
    11701 agcttcgaag attcctgaaa atttattttc agcttattct tagctaatat gttgtttaaa
    11761 cttaccttca agagctcgag tcgagagttg tgatgttgca ctcggggcag caactccgtt
    11821 cttgcgaaca cgacagaaca ggtagttgtt tggatcatcg gcgactccgg aaaccgaaat
    11881 ccagcgctca acgagcttgg taactccaag ggagagcgct ttttcgactc cggcagtcga
    11941 gaccagcgtt tttgtacgtc cgatgtggat gagcattctt cctccgtctg tgcgactaat
    12001 atccttgacg cgaatgcggg caatttcggc gatacggaga agtgtgttgt aagcaattcc
    12061 gaggaaggcg aggtttcgga tgtcctggca acgatcggaa ttttccataa gagaacggac
    12121 ctgatcgaag tcggttctga aaatttcgtc aagtcttagg attgaataca ttaatattta
    12181 aacttacctt tcgaaggcga gggcttgctt ggctctctct ccagcatcaa cattttcctt
    12241 gcggatacgt ctcattacga gagatacggc attagaatct gatgggcgtg gaagtccaga
    12301 tcggcgatgg agcatattga gctgtccgag atgttgttgg atcgtcttca ccgccaatcc
    12361 acgagcttgg aggtagagca agtagtcacg aacgtcttct ggctcggctg ggaaccactt
    12421 gcggttgtta agcttgcacc aggcagccca agaacggcac actgagagaa gcatcttcca
    12481 ggtgtgctcg gagaaggctt gtctgtcacg gaacatatcc atgaggttct tgcgaacttc
    12541 atcacttgtc gcgtcaactg gaagtgctgg aagattttga tgaacagtga gaagatttga
    12601 catattttcg aagtttttta gatgcactag aacaaagcgt gttggcttcc tctgagcccg
    12661 ctttccttat atacccgcat tctgcagcct tacagaatgt tctagaaggt cctagatgca
    12721 ttcgtttgaa aatactcccg gtgggtgcaa agagacgcag acggaaaatg tatctgggtc
    12781 tctttattgt gtacactact tttccatgta ccgaatgtga gtcgccctcc ttttgcaaca
    12841 agcagctcga atgttctaga aaaaggtgga aaatagtata aataccgttg aaaataaata
    12901 ccgaacaaca tttgctctaa ttgtgaaatt agaaatcttc aaactataat catgtcactt
    12961 taccactatt tccgtccagc tcaacgttcc gtttttggtg atcatttttg ctttcgtcgt
    13021 aaatctacac acgcgtctct tccgtgcgag agtccaagcc agcagccaaa ttcgttgact
    13081 gagtattcaa cgtttatacg ttgtcggcaa cgagaaatag gaaaatgcat cgggaaatgt
    13141 tcttttttcg attttttcca aggttttgac aaattttacc acgaattttg ctatgttttc
    13201 aattaaaaaa tatgttattc aactgtttct atgaggaaaa taaggctttg catgtaattt
    13261 tcttattcag cataattttt aattaatttg aattttctgt cctaacgttt attttgtttt
    13321 cttggttatg actgatctga aattaatttt tgaattttaa ggtaatatgt caggcggtgc
    13381 cgcaagtttg tacaaaaaag caggctccat gaaaaagcct gaactcaccg cgacgtctgt
    13441 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
    13501 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
    13561 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
    13621 gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat
    13681 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
    13741 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag
    13801 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
    13861 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
    13921 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
    13981 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
    14041 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa
    14101 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg
    14161 gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct
    14221 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg
    14281 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg
    14341 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg
    14401 acgccccagc actcgtccga gggcaaagga atagacccag ctttcttgta caaagtgggt
    14461 ccaattactc ttcaacatcc ctacatgctc tttctccctg tgctcccacc ccctattttt
    14521 gttattatca aaaaacttct cttaatttct ttgtttttta gcttctttta agtcacctct
    14581 aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa
    14641 ttgtgctccc tccccccatt aataataatt ctatcccaaa atctacacaa tgttctgtgt
    14701 acacttctta tgttttttac ttctgataaa tttttttgaa acatcataga aaaaaccgca
    14761 cacaaaatac cttatcatat gttacgtttc agtttatgac cgcaattttt aataacttcg
    14821 tatagtatac cttatacgaa gttatactag tatatatttt tatgttttct tttaaacctt
    14881 cctccatatt tctgaattga atgttgttgc atattgtgtg tccttatttc atttccatca
    14941 tccttttgtg tgtttccccc ttccaccaat tccccccacg tcctaatgaa atgaaagcga
    15001 gaagataaat aaacttgcac attgtgttca gatgagagga gcggaatacg aattgggaga
    15061 cggaaagaga aaaaaaaatg tttagatttg gattgaatta attttttaga aagcaagaaa
    15121 aaaaacacaa agagaataaa taaataatag gaaaaaaaac aaatattcag tatttaaaat
    15181 ttgaaaattc aacgacgttg gcgtcgatca tcctgtaaaa cgacggccag tgc
//
