LOCUS       sequence-32084-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Villefranc JA, Nicoli S, Bentley K, Jeltsch M, Zarkada G, Moore JC, 
            Gerhardt H, Alitalo K, Lawson ND
  TITLE     A truncation allele in vascular endothelial growth factor c reveals 
            distinct modes of signaling during lymphatic and vascular 
            development.
  JOURNAL   Development. 2013 Apr;140(7):1497-506. doi: 10.1242/dev.084152. Epub
            2013 Mar 5.
  PUBMED    23462469
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.084152"; journalName: "Development"; date: "2013-04"; 
            volume: "140"; issue: "7"; pages: "1497-506"
COMMENT     Sequence Label: 543-p3E-2A-mcherrypA
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(54..177)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             189..245
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(391..409)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     659..678
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cagctctaga gctcgaattc tacaggtcac taataccatc taagtagttg gttcatagtg
       61 actgcatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
      121 atatattgat atttatatca ttttacgttt ctcgttcaac tttcttgtac aaagtggggg
      181 gatccggagc cacgaacttc tctctgttaa agcaagcagg agacgtggaa gaaaaccccg
      241 gtcctatggt gagcaagggc gaggaggaca acatggccat catcaaggag ttcatgcgct
      301 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
      361 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc
      421 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
      481 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
      541 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
      601 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
      661 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
      721 acggcgccct gaa
//
