LOCUS       sequence-320845-       13805 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium
            smegmatis, expresses FnCpf1, NHEJ machinery of M. marinum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13805)
  AUTHORS   Yan MY, Li SS, Ding XY, Guo XP, Jin Q, Sun YC
  TITLE     A CRISPR-Assisted Nonhomologous End-Joining Strategy for Efficient 
            Genome Editing in Mycobacterium tuberculosis.
  JOURNAL   mBio. 2020 Jan 28;11(1). pii: mBio.02364-19. doi: 
            10.1128/mBio.02364-19.
  PUBMED    31992616
REFERENCE   2  (bases 1 to 13805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2020-01-28"; volume: "11(1). pii: mBio.02364-19. doi"; pages:
            " 10.1128/mBio.02364-19"
COMMENT     Sequence Label: pNHEJ-Cas12a
FEATURES             Location/Qualifiers
     source          1..13805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..34
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(51..674)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     582..601
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    5022..5055
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      5121..5709
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5610..5629
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8923..8934
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12569..12588)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             12580..13395
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(12653..12672)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      complement(13696..13742)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ataacttcgt ataatgtatg ctatacgaag ttattaattg cccatggtca ttaagaccca
       61 ctttcacatt taagttgttt ttctaatccg cagatgatca attcaaggcc gaataagaag
      121 gctggctctg caccttggtg atcaaataat tcgatagctt gtcgtaataa tggcggcata
      181 ctatcagtag taggtgtttc cctttcttct ttagcgactt gatgctcttg atcttccaat
      241 acgcaaccta aggtaaaatg ccccacagcg ctgagtgcat ataacgcgtt ctctagtgaa
      301 aaaccttgtt ggcataaaaa ggctaattga ttttcgagag tttcatactg tttttctgta
      361 ggccgtgtac ctaaatgtac ttttgctcca tcgcgatgac ttagtaaagc acatctaaaa
      421 cttttagcgt tattacgcaa aaaatcttgc cagctttccc cttctaaagg gcaaaagtga
      481 gtatggtgcc tatctaacat ctcaatggct aaggcgtcga gcagggctcg cttatttttt
      541 acatgccaat acaatgtagg ctgctctaca ccaagtttct gggcgagttt acgggttgtt
      601 aaaccttcga ttccgacctc attaagcagc tctaatgcgc tgttaatcac tttactttta
      661 tctaatctag acattgcgaa gtgattcctc cgatccgtgg caggagggtc gcctgcgtcg
      721 gcaactcgcc caggctacac gagagccctc gactgcgaaa atgccgacca ggcaccaccg
      781 cactccaggc ttaacccggt gcgacgatcc aatattaata ttggatcgtc ggcaccgtca
      841 cggccgtggg aggcggcacg atccgcgacg tgatgatcgg ccgcatcccc acggtgctgc
      901 gcagtgagct ctacgccatc ccggcgttga tctgtgcgtt cgcacgcaca ggcccggtgt
      961 gagaagggtc tctgacgagc gggagaactc cctatcagtg atagagtttg tcctccctat
     1021 cagtgataga taggctctgg gagtacccgt gtgtacgacc agcacggcat acatcatttc
     1081 gacgccgaga gattcgccgc ccgaaatgag cacgatccat gtcgatctac caagagttcg
     1141 tgaataagta tagcctgagc aagaccttgc ggttcgagtt gatcccgcag ggcaaaacct
     1201 tggaaaacat taaggcgcgc gggctgatcc tggatgatga gaagcgggcc aaggactata
     1261 aaaaggccaa gcagattatc gataaatatc atcagttctt catcgaggaa atcctgtcga
     1321 gcgtgtgcat cagcgaagac ttgttgcaga actatagcga cgtgtacttc aaattgaaaa
     1381 agtcggacga cgacaatctg caaaaagact tcaagtcggc caaagacacc atcaagaaac
     1441 agatttcgga gtacatcaag gactcggaaa agttcaaaaa cctgttcaac cagaacttga
     1501 tcgacgcgaa aaagggccag gaaagcgatt tgatcttgtg gctgaagcag tcgaaggata
     1561 atggcattga gttgttcaag gccaactcgg acattacgga cattgacgag gccctggaaa
     1621 tcattaaatc gtttaagggc tggaccacgt acttcaaggg cttccatgag aaccggaaaa
     1681 acgtgtattc gtcgaacgac attcccacgt cgatcatcta ccggatcgtc gatgacaact
     1741 tgcccaagtt cttggaaaat aaagcgaaat acgagagctt gaaggacaaa gcgccggagg
     1801 ccattaacta tgagcagatc aagaaggacc tggccgaaga gctgaccttt gacattgact
     1861 acaagaccag cgaggtcaat cagcgggtct tctcgttgga tgaggtcttt gaaatcgcca
     1921 atttcaacaa ctatttgaac cagagcggca tcaccaaatt taataccatt atcggcggga
     1981 agttcgtcaa cggcgagaac acgaagcgga aggggatcaa cgaatacatc aatctgtact
     2041 cgcagcagat caacgacaag accctgaaaa agtacaagat gagcgtgctg ttcaagcaaa
     2101 ttctgagcga caccgagagc aagtcgttcg tcattgacaa gctggaggat gacagcgatg
     2161 tggtcaccac gatgcagtcg ttttacgagc aaatcgcggc cttcaagacc gtggaggaaa
     2221 agagcatcaa ggaaaccctg agcctgctgt tcgatgatct gaaggcgcag aagttggatc
     2281 tgtcgaaaat ctacttcaag aacgataagt cgctgaccga cctgagccaa caggtcttcg
     2341 atgattacag cgtcatcggg accgccgtcc tggagtacat cacccagcag attgcgccca
     2401 aaaacctgga caatccctcg aaaaaagagc aggagctgat tgccaagaag accgagaagg
     2461 cgaagtactt gtcgctggaa accatcaaac tggcgctgga ggagttcaac aagcaccgcg
     2521 acattgataa acaatgccgg ttcgaggaga ttctggccaa ttttgccgcc attcccatga
     2581 tcttcgatga gatcgcgcag aataaagaca acctggcgca aatctcgatc aaatatcaga
     2641 atcagggcaa aaaagacctg ttacaagcct cggccgaaga cgacgtgaaa gccatcaagg
     2701 acctgttgga tcagacgaac aacttgttgc acaagctgaa aattttccac atcagccaga
     2761 gcgaggacaa ggccaacatc ttggacaaag acgagcactt ctacttggtg ttcgaagagt
     2821 gctacttcga attggccaac atcgtccccc tgtacaataa gattcgcaat tatattacgc
     2881 agaagcccta tagcgacgag aaattcaagt tgaattttga aaattcgacc ctggcgaatg
     2941 gctgggacaa gaacaaagag cccgacaaca ccgccatctt gttcatcaag gatgataaat
     3001 attatctggg ggtgatgaac aaaaagaata acaagatctt tgatgacaag gccatcaagg
     3061 agaataaggg cgaggggtac aagaaaatcg tctacaaact gttgcccggc gccaacaaga
     3121 tgttgccgaa ggtcttcttc agcgcgaaga gcattaaatt ctataacccc agcgaggaca
     3181 tcctgcgcat ccggaatcat tcgacccaca cgaagaatgg cagcccgcaa aagggctacg
     3241 agaagttcga gtttaacatc gaggattgtc ggaaattcat cgacttctat aaacagtcga
     3301 tcagcaagca ccccgagtgg aaggatttcg gcttccgctt cagcgatacc caacgctaca
     3361 acagcatcga cgagttttat cgggaagtgg aaaaccaggg gtacaagttg accttcgaga
     3421 acatttcgga gtcgtacatt gactcggtcg tcaaccaagg gaaactgtac ctgttccaaa
     3481 tctacaataa ggacttcagc gcgtactcga aagggcggcc caacttgcac accctgtatt
     3541 ggaaggcctt gtttgacgag cggaacttac aagacgtcgt ctataaactg aacggggagg
     3601 cggagctgtt ttaccgcaag cagtcgatcc cgaaaaagat tacccacccg gcgaaggagg
     3661 cgatcgccaa caaaaacaag gacaacccga agaaagagtc ggtcttcgaa tacgatctga
     3721 tcaaagacaa gcgctttacc gaggacaagt tctttttcca ttgtccgatc accatcaatt
     3781 ttaagagcag cggcgcgaac aagttcaacg acgagatcaa cctgctgttg aaagaaaagg
     3841 ccaatgatgt gcacatcctg tcgatcgacc ggggggaacg gcacctggcc tactacaccc
     3901 tggtggacgg caagggcaac atcatcaagc aggatacctt caatatcatt gggaacgacc
     3961 ggatgaaaac gaactaccac gacaagctgg cggccatcga aaaggatcgc gacagcgccc
     4021 gcaaggactg gaagaagatt aacaatatca aggagatgaa ggaggggtac ttgagccagg
     4081 tcgtgcacga gatcgccaag ctggtcattg agtacaacgc gattgtggtc tttgaagacc
     4141 tgaactttgg cttcaaacgg gggcggttca aggtcgagaa gcaggtgtat cagaagctgg
     4201 aaaaaatgtt gatcgagaag ctgaactatc tggtgttcaa agacaacgag tttgacaaaa
     4261 cggggggcgt cctgcgggcc taccagttga ccgccccctt cgaaaccttc aagaaaatgg
     4321 gcaagcagac gggcatcatc tactacgtcc cggcgggctt tacgagcaag atctgtccgg
     4381 tgaccggctt cgtcaaccag ctgtatccga agtatgagag cgtgtcgaaa agccaggagt
     4441 ttttctcgaa gttcgataaa atctgttata acctggataa ggggtacttc gaattttcgt
     4501 tcgattacaa aaacttcggg gacaaggcgg ccaaggggaa atggacgatc gccagcttcg
     4561 gctcgcgcct gattaacttt cgcaactcgg acaagaacca taactgggac acccgggaag
     4621 tgtacccgac caaagaattg gaaaaactgt tgaaggacta tagcatcgag tacgggcacg
     4681 gggagtgcat caaagccgcc atctgcgggg aaagcgacaa gaagtttttt gcgaagctga
     4741 ccagcgtgct gaatacgatc ttacaaatgc gtaattctaa gaccggcacc gagctggact
     4801 acctgatctc gccggtggcg gatgtcaacg gcaacttctt cgacagccgc caggccccga
     4861 agaacatgcc gcaggacgcg gatgcgaatg gcgcgtacca catcgggctg aaagggctga
     4921 tgctgctggg gcggatcaag aacaaccagg aggggaagaa gctgaatctg gtgattaaga
     4981 acgaggaata tttcgagttc gtccaaaatc gcaacaattg aataacttcg tataatgtat
     5041 gctatacgaa gttattaatt ctgcagagtc ctactagttc catgagcgtc agaccccgta
     5101 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5161 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5221 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     5281 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5341 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5401 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5461 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5521 agcgccacgc ttcccgaggg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5581 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5641 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5701 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5761 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     5821 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcaacgcgtg agcccaccag
     5881 ctccgtaagt tcgggcgctg tgtggctcgt acccgcgcat tcaggcggca gggggtctaa
     5941 cgggtctaag gcggcgtgta cggccgccac agcggctctc agcggcccgg aaacgtcctc
     6001 gaaacgacgc atgtgttcct cctggttggt acaggtggtt gggggtgctc ggctgtcgct
     6061 ggtgttccac caccagggct cgacgggaga gcgggggagt gtgcagttgt ggggtggccc
     6121 ctcagcgaaa tatctgactt ggagctcgtg tcggaccata caccggtgat taatcgtggt
     6181 ctactaccaa gcgtgagcca cgtcgccgac gaatttgagc agctctggct gccgtactgg
     6241 ccgctggcaa gcgacgatct gctcgagggg atctaccgcc aaagccgcgc gtcggcccta
     6301 ggccgccggt acatcgaggc gaacccaaca gcgctggcaa acctgctggt cgtggacgta
     6361 gaccatccag acgcagcgct ccgagcgctc agcgcccggg ggtcccatcc gctgcccaac
     6421 gcgatcgtgg gcaatcgcgc caacggccac gcacacgcag tgtgggcact caacgcccct
     6481 gttccacgca ccgaatacgc gcggcgtaag ccgctcgcat acatggcggc gtgcgccgaa
     6541 ggccttcggc gcgccgtcga tggcgaccgc agttactcag gcctcatgac caaaaacccc
     6601 ggccacatcg cctgggaaac ggaatggctc cactcagatc tctacacact cagccacatc
     6661 gaggccgagc tcggcgcgaa catgccaccg ccgcgctggc gtcagcagac cacgtacaaa
     6721 gcggctccga cgccgctagg gcggaattgc gcactgttcg attccgtcag gttgtgggcc
     6781 tatcgtcccg ccctcatgcg gatctacctg ccgacccgga acgtggacgg actcggccgc
     6841 gcgatctatg ccgagtgcca cgcgcgaaac gccgaatttc cgtgcaacga cgtgtgtccc
     6901 ggaccgctac cggacagcga ggtccgcgcc atcgccaaca gcatttggcg ttggatcaca
     6961 accaagtcgc gcatttgggc ggacgggatc gtggtctacg aggccacact cagtgcgcgc
     7021 cagtcggcca tctcgcggaa gggcgcagca gcgcgcacgg cggcgagcac agttgcgcgg
     7081 cgcgcaaagt ccgcgtcagc catggaggca ttgctatgag cgacggctac agcgacggct
     7141 acaaccggca gccgactgtc cgcaaaaagc ggcgcgtgac cgccgccgaa ggcgctcgaa
     7201 tcaccggact atccgaacgc cacgtcgtcc ggctcgtggc gcaggaacgc agcgagtggc
     7261 tcgccgagca ggctgcacgc cgcgaacgca tccgcgccta tcacgacgac gagggccact
     7321 cttggccgca aacggccaaa catttcgggc tgcatctgga caccgttaag cgactcggct
     7381 atcgggcgag gaaagagcgt gcggcagaac aggaagcggc tcaaaaggcc cacaacgaag
     7441 ccgacaatcc accgctgttc taacgcaatt ggggagcggg tgtcgcgggg gttccgtggg
     7501 gggttccgtt gcaacgggtc ggacaggtaa aagtcctggt agacgctagt tttctggttt
     7561 gggccatgcc tgtctcgttg cgtgtttcgt tgcgcccgtt ttgaatacca gccagacgag
     7621 acggggttct acgaatcttg gtcgatacca agccatttcc gctgaatatc ggggagctca
     7681 ccgccagaat cggtggttgt ggtgatgtac gtggcgaact ccgttgtagt gcctgtggtg
     7741 gcatccgtgg cgcggccgcg gtacccaaga agcacagtga gtcgcggatc gatggcgggc
     7801 gcggtatcgg cgccgccaac gtcacctcgt cgagagccac cacctcactc ggtgagcgca
     7861 agacgttttc accggcttcg cgtaatccgt cggcgccgcg cttgatcaaa tcgagcaggg
     7921 cgactcccgg cgccatggcg tagatcgaac cgtcggacaa caccccgaca cgctcaccgt
     7981 ccgcacttcg atacgtcacc catttcattc gcgcaccacc tcactcgggt ttttgtccgg
     8041 gcgcaaccca cgccagctgg attgccgcaa tcggtcatcg ggggtccact cgctatagcg
     8101 cacctccccg accagcgtcg gcgcgacgaa cgtaacaccc ttggcctcgc gcgccggaag
     8161 cgcagcgtcg aatggggatt cgtcggtatg cagcggcgcc agcgtcttct tgaggttggc
     8221 caggtcacgt tcggtgaagc cggttccaac acggccggcg aagtgcaaac cgctcgggcc
     8281 gggaatgccc atgagcaacg atccaatccc gctgctgcgc ccaccctccc caactcgcca
     8341 accgccgatg acgacttcct gggtgttcca gtgtttgtcc ttgatccacg ccgacgagcg
     8401 ccgcccgggt tgatagccgg agtcgcggcg cttggcaatg actccctccc aaccgcgctt
     8461 gtgcgagtag gccagcgcct cggcgccgtc accgggcaac aactccggaa cgatcagatt
     8521 gcccgcactg cccagggttt ccagtagctt gcgccggtcg cggtatctgg cccgcagtag
     8581 cgagcgaccg tccagaaaga gcaagtcgaa cgcccagaac tcgatgcggg tggcgcgcac
     8641 ccggttctgc atctcgttga agctgggcac tccggaggag tcgagggcca ccaattcccc
     8701 gtcgagcacc acgtggtgat cagcgagatc ggcggccaat gaacgtaatt gggggtattc
     8761 cgcggtgacg tcacgtccgc tgcgggagcg cagtcgcagc gcgccatggt cggcttcgac
     8821 cagcagccgg tagccgtccc acttgccttc gaacgcccac tgacccgact cgagacctgc
     8881 caccgaaccg tgggaggtga gcatgggcgc gagggtgtcg aaatcgaagg ccgtctggtc
     8941 cttcatgcga tgcgccagcc attggtcccc gttggtctga atcagcgcgt accgccccga
     9001 gattcgactg ccgtgcaggt tgacaatcac ctcccccttt tccgaaccgt ttctcgagtc
     9061 gtcgcggaac ttctcggtgt cataggtgcc ggaatcccag atgatcacct tgccggcgcc
     9121 gtactcccct ttcgggatgt tgccctcgaa cgtcgcgtat tccagcggat gatcttcggt
     9181 gtgcacggcc aggtggttca ccgacgtggt ttccggcagg tttttcggca ccgcccatga
     9241 caccagtacc ccatcacgct ccagcctgaa gtcgtaatgc agacggcgcg cgtgatgctc
     9301 ttggatcacg aaggtattac cctgcccgac aacgggtctg gctcggggca ccggctccgg
     9361 cgttttcgac gcgttgcgca tgctgcgata tttgagcagc cggtcgtcgc cggggaggtc
     9421 cgcgtccagc gccgcaatca gatcgccatc gcgggtgagg cgggcaagca cctcgtcgta
     9481 ggtcagctgg cgcagccccg ggtcgtcgag ctcctcccag ctgcgtggtg cggcgacggt
     9541 cggttgttcg cgcccacgca gcgaatacgg cgcgatggtg gtcttcgaac cgctgttttg
     9601 gctccagtcc aggaacactt tcccggcccg cagactcttg gtcatggtcg cggtgaccaa
     9661 cgccggcatc accgtttcca gttgctgcgc gacccgtttg gccaacaccg tggcgccctt
     9721 gctgctcacc ggctcgtcga gcggggtgta gaggtgcagg cccttgctac cgctggtgag
     9781 cgggagggta ctcaggccga tgtcggcgag cagatcgcgc accgcccgcg ccacctgcgc
     9841 gagttgggcc atcgtcacgc cctcgcccgg gtccaggtcg aacaccaatc gggtggccgg
     9901 cccgggcgtg aggatttcgc ctttcgacgt ccactcggca acgaaccgcc actgcgggac
     9961 gtgcacctcg agggcggcct gttgcgcgat ccaggccagc ccggtggcgc tgtcgatgat
    10021 cggataggtc gtcgttcccg accggtgcac cacgctggca cgcggcagcc agtccggcgc
    10081 cgacgacgcc agctgctttt cgaaaaagga cgcctgatcg acgccgttgg gccagcgttt
    10141 gcgagtcgcc gggcgcccgg cgatatgcgg gatcatcacc tcggcgatgc cggtgtagta
    10201 accgaagacg tcagacttgg tggttccggt agccgggtac agcaccttgt ccgcgttggt
    10261 cagtgtcacc tgcggaccat tcgcctgtcc aggaggccag gcctgcgccg aacccatatg
    10321 gccaacgtaa cctgtcgcca tggcacgtgg gctggtgatc ggtgaagccc tgatcgacat
    10381 cgtcgatggg ccggacccgg ccgagtatgt cgggggcagc cccctcaacg tcgcggtcgg
    10441 actggccaga ttgggccgcg acgtcgatct gctgacccac atcggccgcg acgcccgcgg
    10501 acgacggatc gccgagtaca ttgaatcctc aggtgtacaa cttgtttcgg gaagccagac
    10561 ggctgatcgg acgcccaccg cgacggccag gatcggcccc gacggctcgg ccacctacgc
    10621 gttcgacctg gaatggcaga tacccgacac accccccgtg gcgccgccgc ttctcgtgca
    10681 caccggttcc atcgccgccg cgcgggaacc aggatgcttg gcggtcgcgg cgctgctcga
    10741 cgcctatcgg gccgcggcca cggtgagctt cgatcccaat gtgcgtccgt cgctgagcgc
    10801 cgacccggac ctgactcggg aacgcatcca acgcctcgtc gagcgcagcg acatcatcaa
    10861 ggccagtgcc gaagacttgc actggatcga ccccacccag cctccggagc agaccgcgcg
    10921 ggcctggctg gcctgcggcc cggcgattgt cgcgctgacg ttgggcgatc agggcgcagt
    10981 ggcgttctgc gcggccggcc cggcaagtgt gcccgcccag ccggttcgcg tggtcgatac
    11041 cgtcggcgcc ggagacgcgt tcatggccgg tctgctcgac acgctgtggg agcagggatt
    11101 gttaggtgcc gaccggcgca ccgagttgcg caagatcggc gtgtcggcgc tgacctctgc
    11161 actcgaagtc gcggccctga cctcggcgtt gaccgttgcc cgtgccggcg ccgatctgcc
    11221 ctatcgggcc gacctgcggc agtcgcgatg accaccgcac gcccgcaatc gggcccggcg
    11281 agggtgcacg aaatgccgcg tgatgcggcg tgtcgccgtg ccgacacgca cgctcgcgag
    11341 ctgaccgggg ctggggcagg cagcaggact ccggtgccgc ggcgcactgt taaccgaccc
    11401 gggttctggg catactgact ccatgcgctc catctggaag ggttcaatcg ctttcgggct
    11461 ggtgaatgtg ccggtcaagg tgtacagcgc cactcaggac cacgacatca agttccatca
    11521 ggtgcacgcc aaggacaacg gacgcatccg gtacaagcgg gtatgcgagg tctgtggcga
    11581 ggtggtcgaa tactccgacc tggcccgcgc cttcgaatcc gacgatggcc agatggtgat
    11641 catcaacgac gacgacctcg ccacgttgcc cgaagatcgc agccgcgaaa tcgaggtgct
    11701 ggaattcgtc ccagccgaag aagtcgatcc gatgatgatt gaccgcagct acttcctgga
    11761 gcccgactcg aagtcctcta aatcgtatgt gctgctggcc aagacgctcg ccgagacgga
    11821 gcggatggcg atcgtgcatt tcacgctgcg cagcaagacg cggctggcgg cgttgcgggt
    11881 caaggacttc ggcaagcgcg aggtcatggt ggtgcatacc ctgttgtggc cagacgagat
    11941 ccgcgacccg gatttcccgg tgctggacaa ggaggtggag atcaaacccg cggagctcaa
    12001 gatggccggc caagtggtgg agtcgatggc agacgacttc aacccggacc gctaccgcga
    12061 cacctatcag gagcaactgc aggagctgat cgacgccaaa ctcgaaggcg gggaagcatt
    12121 taccgccgaa gaacaacccg cagagctgga cgagaccgaa gatgtctccg acctgctggc
    12181 caagctggag gcgagcgtga aagcgcgttc gggcgacggg aagtcagcgg cgaagaagag
    12241 caccgccgaa aaggcgccgg ccaagaaggc ccgggccaaa caagctgcca agtcctgacc
    12301 agcgcccgac ttgttcacat atgctcgtcg tgccgccgag aggagttttt gcgtgaagtc
    12361 caaccgatct ggcgtcgtgc tcagcctgct gctcgccagc acattggcgt tgtcggcatg
    12421 cggaggccat tccagcaata cctcgtccac ctgacgtcgg ggggggggga aagccacgtt
    12481 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
    12541 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
    12601 acgtcttgct cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
    12661 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
    12721 catgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
    12781 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
    12841 atccgtactc ctgatgatgc atggttactc accactgcga tccccgggaa aacagcattc
    12901 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
    12961 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
    13021 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
    13081 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa tcttttgcca
    13141 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
    13201 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
    13261 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
    13321 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
    13381 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
    13441 acttgacggg acggcggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga
    13501 tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca
    13561 actggtccac ctacaacaaa gctctcacca accgtggctc cctcactttc tggctggatg
    13621 atggggcgat tcaggcctgg tatgagtcag caacaccttc ttcacgaggc agacctcact
    13681 agtggccatg atggcataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
    13741 tgatgcctgg cagtcgatcg tacgctagtt aactacgtcg acatcgataa gcttggcgtg
    13801 aattc
//
