LOCUS       sequence-320857-        9595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EGFP reporter plasmid containing 25 tandem repeats of the the 
            unfolded protein response element (UPRE).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Kitakaze K, Taniuchi S, Kawano E, Hamada Y, Miyake M, Oyadomari M, 
            Kojima H, Kosako H, Kuribara T, Yoshida S, Hosoya T, Oyadomari S
  TITLE     Cell-based HTS identifies a chemical chaperone for preventing ER 
            protein aggregation and proteotoxicity.
  JOURNAL   Elife. 2019 Dec 17;8. pii: 43302. doi: 10.7554/eLife.43302.
  PUBMED    31843052
REFERENCE   2  (bases 1 to 9595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-12-17"; volume: "8. pii: 43302. doi"; pages: " 
            10.7554/eLife.43302"
COMMENT     Sequence Label: lenti-25xUPRE-minP-EGFP
FEATURES             Location/Qualifiers
     source          1..9595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3212..3243
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      3270..3279
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3315..3324
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3321..3323,3324..3326,3327..4040)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3321..3323,3324..3326,3327..4037)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3366..3387)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3627..3646)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3974..3995
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4071..4659
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4124..4144)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4542..4553)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4662..4678)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4663..4679)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5184..5364
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5390..5407)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5396..5620
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5666..6094
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5753..5772)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5963..5984
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6103..6123)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6268..6402
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6329..6348
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6464..6511
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6530..6904
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7034..7155
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7071..7090)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7125..7144
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7204..7220)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7204..7220)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7217..7239)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7228..7244
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7252..7258,7259..7276,7277..7282))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7297..7318
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7435..7452)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7606..8194)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7686..7705)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8365..9156,9157..9225))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8988..9007
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9226..9330)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9405..9424)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttagctagcc tctctcgaga caggtgctga cgtggcattc
     2401 tctactcgag acaggtgctg acgtggcatt cgtatctcga gacaggtgct gacgtggcat
     2461 tcgaggctcg agacaggtgc tgacgtggca ttccgacctc gagacaggtg ctgacgtggc
     2521 attctacatc agctctctcg agacaggtgc tgacgtggca ttctctactc gagacaggtg
     2581 ctgacgtggc attcgtatct cgagacaggt gctgacgtgg cattcgaggc tcgagacagg
     2641 tgctgacgtg gcattccgac ctcgagacag gtgctgacgt ggcattctac aactcctctc
     2701 tcgagacagg tgctgacgtg gcattctcta ctcgagacag gtgctgacgt ggcattcgta
     2761 tctcgagaca ggtgctgacg tggcattcga ggctcgagac aggtgctgac gtggcattcc
     2821 gacctcgaga caggtgctga cgtggcattc tacagtctct ctctcgagac aggtgctgac
     2881 gtggcattct ctactcgaga caggtgctga cgtggcattc gtatctcgag acaggtgctg
     2941 acgtggcatt cgaggctcga gacaggtgct gacgtggcat tccgacctcg agacaggtgc
     3001 tgacgtggca ttctacaacg actctctcga gacaggtgct gacgtggcat tctctactcg
     3061 agacaggtgc tgacgtggca ttcgtatctc gagacaggtg ctgacgtggc attcgaggct
     3121 cgagacaggt gctgacgtgg cattccgacc tcgagacagg tgctgacgtg gcattctaca
     3181 agatctggcc tcggcggcca agcttagaca ctagagggta tataatggaa gctcgacttc
     3241 cagcttggca atccggtact gttggtaaag ccaccatgga agatgccaaa aacattaaga
     3301 aggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     3361 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     3421 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     3481 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     3541 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     3601 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     3661 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     3721 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     3781 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     3841 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     3901 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     3961 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     4021 tggacgagct gtacaagtaa agcggccaat tcgatatcaa gcttatcggt aatcaacctc
     4081 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     4141 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     4201 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     4261 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     4321 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     4381 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     4441 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     4501 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     4561 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     4621 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag
     4681 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     4741 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     4801 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     4861 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     4921 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     4981 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     5041 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     5101 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     5161 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     5221 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     5281 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     5341 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg
     5401 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     5461 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     5521 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     5581 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     5641 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5701 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     5761 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5821 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5881 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     5941 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     6001 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     6061 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     6121 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     6181 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     6241 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccagt tccgcccatt
     6301 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     6361 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     6421 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
     6481 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt
     6541 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac
     6601 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga
     6661 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc
     6721 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac
     6781 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg
     6841 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
     6901 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     6961 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     7021 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7081 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7141 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     7201 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7261 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     7321 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7381 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7441 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7501 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7561 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7621 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7681 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7741 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7801 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7861 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7921 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7981 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8041 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8101 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8161 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8221 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8281 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8341 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8401 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8461 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8521 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8581 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8641 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8701 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8761 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8821 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8881 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8941 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9001 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9061 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9121 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9181 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9241 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9301 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9361 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     9421 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     9481 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
     9541 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgtt
//
