LOCUS       sequence-320861-        2097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 N-terminal tag  part for MoClo assembly, CCAT-AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Dudley QM, Cai YM, Kallam K, Debreyne H, Carrasco Lopez JA, Patron 
            NJ
  TITLE     Biofoundry-assisted expression and characterization of plant 
            proteins.
  JOURNAL   Synth Biol (Oxf). 2021 Sep 11;6(1):ysab029. doi: 
            10.1093/synbio/ysab029. eCollection 2021.
  PUBMED    34693026
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-09-11"; volume: "6(1):ysab029. doi"; pages: " 
            10.1093/synbio/ysab029. eCollection 2021"
COMMENT     Sequence Label: pEPYC0CM0258
FEATURES             Location/Qualifiers
     source          1..2097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..185
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     413..431
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(435..478)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      551..608
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(632..649)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(803..1391)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(883..902)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1574..1668)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1689..2097,1..251))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg
       61 gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa
      121 aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat
      181 gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt
      241 tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc
      301 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaactcg
      361 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
      421 tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg
      481 gaattcggtc tcaccatggt gagcggctgg cggctgttca agaagattag cggaatgcga
      541 gaccctgcag tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac
      601 cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg
      661 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      721 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      781 aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa
      841 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      901 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      961 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     1021 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     1081 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     1141 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     1201 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     1261 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1321 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1381 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1441 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1501 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1561 gctcgaggct tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
     1621 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg
     1681 atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     1741 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     1801 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     1861 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     1921 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     1981 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     2041 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccat
//
