LOCUS       sequence-320863-        2391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 N-terminal tag  part for MoClo assembly, CCAT-AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Dudley QM, Cai YM, Kallam K, Debreyne H, Carrasco Lopez JA, Patron 
            NJ
  TITLE     Biofoundry-assisted expression and characterization of plant 
            proteins.
  JOURNAL   Synth Biol (Oxf). 2021 Sep 11;6(1):ysab029. doi: 
            10.1093/synbio/ysab029. eCollection 2021.
  PUBMED    34693026
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-09-11"; volume: "6(1):ysab029. doi"; pages: " 
            10.1093/synbio/ysab029. eCollection 2021"
COMMENT     Sequence Label: pEPQD0CM0550
FEATURES             Location/Qualifiers
     source          1..2391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             375..395
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(463..479)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(463..479)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(476..498)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(651..1239)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(731..750)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1372..1466)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1487..2146)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2061..2080
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2147..2249)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2336..2358
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2350..2367
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2351..2367
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aataaggaag tgccattccg cctgacctag gtctcaccat ggtaagcggc tggcgtctgt
       61 tcaagaaaat tagcggatca gatagtgaag tgaaccaaga agcaaaacca gaggtcaagc
      121 cggaagttaa accggaaacc catattaacc tgaaagttag cgacggtagc tcggaaattt
      181 tctttaaaat taagaaaact acgccgctgc gtcgcttaat ggaagcattt gccaagcgtc
      241 aaggcaaaga gatggatagc ctgcgctttc tttatgatgg catccgcatc caggcggacc
      301 aaactccaga ggacttagat atggaagata atgacatcat cgaagcccat cgtgagcaaa
      361 tcggcggcat tgaagagaac ctgtattttc agtcaatgag agacctaggc taggtggagg
      421 ctcagtgatg ataagtctgc gatggtggat gcatgtgtca tggtcatagc tgtttcctgt
      481 gtgaaattgt tatccgctca gagggcacaa tcctattccg cgctatccga caatctccaa
      541 gacattaggt ggagttcagt tcggcgtatg gcatatgtcg ctggaaagaa catgtgagca
      601 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
      661 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      721 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      781 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      841 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      901 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      961 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1021 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1081 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1141 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1201 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1261 acggggtctg acgctctatt caacaaagcc gccgtcccgt caagtcagcg taaatgggta
     1321 gggggcttca aatcgtcccc ccatacgata taagttgtta ctagtgcttg gattctcacc
     1381 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
     1441 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
     1501 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     1561 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     1621 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     1681 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
     1741 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     1801 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     1861 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     1921 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     1981 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     2041 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     2101 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     2161 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     2221 gaaagttgga acctcttacg tgccgatcaa cgtctcattg atacctgaaa caaaacccat
     2281 cgtacggcca aggaagtctc caataactgt gatccaccac aagcgccagg gttttcccag
     2341 tcacgacgtt gtaaaacgac ggccagtcat gcataatccg cacgcatctg g
//
