LOCUS       sequence-320864-        2355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 N-terminal tag  part for MoClo assembly, CCAT-AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Dudley QM, Cai YM, Kallam K, Debreyne H, Carrasco Lopez JA, Patron 
            NJ
  TITLE     Biofoundry-assisted expression and characterization of plant 
            proteins.
  JOURNAL   Synth Biol (Oxf). 2021 Sep 11;6(1):ysab029. doi: 
            10.1093/synbio/ysab029. eCollection 2021.
  PUBMED    34693026
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-09-11"; volume: "6(1):ysab029. doi"; pages: " 
            10.1093/synbio/ysab029. eCollection 2021"
COMMENT     Sequence Label: pEPQD0CM0549
FEATURES             Location/Qualifiers
     source          1..2355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             331..351
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(419..435)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(419..435)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(432..454)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(607..1195)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(687..706)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1328..1422)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1443..2102)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2017..2036
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2103..2205)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2292..2314
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2306..2323
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2307..2323
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agtgccattc cgcctgacct aggtctctcc atgtcagata gtgaagtgaa ccaagaagca
       61 aaaccagagg tcaagccgga agttaaaccg gaaacccata ttaacctgaa agttagcgac
      121 ggtagctcgg aaattttctt taaaattaag aaaactacgc cgctgcgtcg cttaatggaa
      181 gcatttgcca agcgtcaagg caaagagatg gatagcctgc gctttcttta tgatggcatc
      241 cgcatccagg cggaccaaac tccagaggac ttagatatgg aagataatga catcatcgaa
      301 gcccatcgtg agcaaatcgg cggcattgaa gagaacctgt attttcagtc aatgagagac
      361 ctaggctagg tggaggctca gtgatgataa gtctgcgatg gtggatgcat gtgtcatggt
      421 catagctgtt tcctgtgtga aattgttatc cgctcagagg gcacaatcct attccgcgct
      481 atccgacaat ctccaagaca ttaggtggag ttcagttcgg cgtatggcat atgtcgctgg
      541 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      601 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      661 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      721 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      781 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
      841 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
      901 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
      961 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     1021 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     1081 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1141 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1201 tcctttgatc ttttctacgg ggtctgacgc tctattcaac aaagccgccg tcccgtcaag
     1261 tcagcgtaaa tgggtagggg gcttcaaatc gtccccccat acgatataag ttgttactag
     1321 tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
     1381 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca
     1441 aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
     1501 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
     1561 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
     1621 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata
     1681 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
     1741 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
     1801 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
     1861 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
     1921 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
     1981 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
     2041 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
     2101 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     2161 cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattgatac
     2221 ctgaaacaaa acccatcgta cggccaagga agtctccaat aactgtgatc caccacaagc
     2281 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtcatgcat aatccgcacg
     2341 catctggaat aagga
//
