LOCUS       sequence-320865-       14001 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR system used for genome editing in Mycobacterium smegmatis, 
            expresses codon-optimized FnCpf1 and the recombination proteins gp60
            and gp61.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14001)
  AUTHORS   Yan MY, Yan HQ, Ren GX, Zhao JP, Guo XP, Sun YC
  TITLE     CRISPR-Cas12a-Assisted Recombineering in Bacteria.
  JOURNAL   Appl Environ Microbiol. 2017 Aug 17;83(17). pii: AEM.00947-17. doi: 
            10.1128/AEM.00947-17. Print 2017 Sep 1.
  PUBMED    28646112
REFERENCE   2  (bases 1 to 14001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2017-08-17"; volume: "83(17). pii: 
            AEM.00947-17. doi"; pages: " 10.1128/AEM.00947-17. Print 2017 Sep 1"
COMMENT     Sequence Label: pJV53-Cas12a
FEATURES             Location/Qualifiers
     source          1..14001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..34
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(51..674)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     582..601
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    5022..5055
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      5121..5709
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5610..5629
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      12569..12615
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(12765..12784)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             12776..13591
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(12849..12868)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      complement(13892..13938)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ataacttcgt ataatgtatg ctatacgaag ttattaattg cccatggtca ttaagaccca
       61 ctttcacatt taagttgttt ttctaatccg cagatgatca attcaaggcc gaataagaag
      121 gctggctctg caccttggtg atcaaataat tcgatagctt gtcgtaataa tggcggcata
      181 ctatcagtag taggtgtttc cctttcttct ttagcgactt gatgctcttg atcttccaat
      241 acgcaaccta aggtaaaatg ccccacagcg ctgagtgcat ataacgcgtt ctctagtgaa
      301 aaaccttgtt ggcataaaaa ggctaattga ttttcgagag tttcatactg tttttctgta
      361 ggccgtgtac ctaaatgtac ttttgctcca tcgcgatgac ttagtaaagc acatctaaaa
      421 cttttagcgt tattacgcaa aaaatcttgc cagctttccc cttctaaagg gcaaaagtga
      481 gtatggtgcc tatctaacat ctcaatggct aaggcgtcga gcagggctcg cttatttttt
      541 acatgccaat acaatgtagg ctgctctaca ccaagtttct gggcgagttt acgggttgtt
      601 aaaccttcga ttccgacctc attaagcagc tctaatgcgc tgttaatcac tttactttta
      661 tctaatctag acattgcgaa gtgattcctc cgatccgtgg caggagggtc gcctgcgtcg
      721 gcaactcgcc caggctacac gagagccctc gactgcgaaa atgccgacca ggcaccaccg
      781 cactccaggc ttaacccggt gcgacgatcc aatattaata ttggatcgtc ggcaccgtca
      841 cggccgtggg aggcggcacg atccgcgacg tgatgatcgg ccgcatcccc acggtgctgc
      901 gcagtgagct ctacgccatc ccggcgttga tctgtgcgtt cgcacgcaca ggcccggtgt
      961 gagaagggtc tctgacgagc gggagaactc cctatcagtg atagagtttg tcctccctat
     1021 cagtgataga taggctctgg gagtacccgt gtgtacgacc agcacggcat acatcatttc
     1081 gacgccgaga gattcgccgc ccgaaatgag cacgatccat gtcgatctac caagagttcg
     1141 tgaataagta tagcctgagc aagaccttgc ggttcgagtt gatcccgcag ggcaaaacct
     1201 tggaaaacat taaggcgcgc gggctgatcc tggatgatga gaagcgggcc aaggactata
     1261 aaaaggccaa gcagattatc gataaatatc atcagttctt catcgaggaa atcctgtcga
     1321 gcgtgtgcat cagcgaagac ttgttgcaga actatagcga cgtgtacttc aaattgaaaa
     1381 agtcggacga cgacaatctg caaaaagact tcaagtcggc caaagacacc atcaagaaac
     1441 agatttcgga gtacatcaag gactcggaaa agttcaaaaa cctgttcaac cagaacttga
     1501 tcgacgcgaa aaagggccag gaaagcgatt tgatcttgtg gctgaagcag tcgaaggata
     1561 atggcattga gttgttcaag gccaactcgg acattacgga cattgacgag gccctggaaa
     1621 tcattaaatc gtttaagggc tggaccacgt acttcaaggg cttccatgag aaccggaaaa
     1681 acgtgtattc gtcgaacgac attcccacgt cgatcatcta ccggatcgtc gatgacaact
     1741 tgcccaagtt cttggaaaat aaagcgaaat acgagagctt gaaggacaaa gcgccggagg
     1801 ccattaacta tgagcagatc aagaaggacc tggccgaaga gctgaccttt gacattgact
     1861 acaagaccag cgaggtcaat cagcgggtct tctcgttgga tgaggtcttt gaaatcgcca
     1921 atttcaacaa ctatttgaac cagagcggca tcaccaaatt taataccatt atcggcggga
     1981 agttcgtcaa cggcgagaac acgaagcgga aggggatcaa cgaatacatc aatctgtact
     2041 cgcagcagat caacgacaag accctgaaaa agtacaagat gagcgtgctg ttcaagcaaa
     2101 ttctgagcga caccgagagc aagtcgttcg tcattgacaa gctggaggat gacagcgatg
     2161 tggtcaccac gatgcagtcg ttttacgagc aaatcgcggc cttcaagacc gtggaggaaa
     2221 agagcatcaa ggaaaccctg agcctgctgt tcgatgatct gaaggcgcag aagttggatc
     2281 tgtcgaaaat ctacttcaag aacgataagt cgctgaccga cctgagccaa caggtcttcg
     2341 atgattacag cgtcatcggg accgccgtcc tggagtacat cacccagcag attgcgccca
     2401 aaaacctgga caatccctcg aaaaaagagc aggagctgat tgccaagaag accgagaagg
     2461 cgaagtactt gtcgctggaa accatcaaac tggcgctgga ggagttcaac aagcaccgcg
     2521 acattgataa acaatgccgg ttcgaggaga ttctggccaa ttttgccgcc attcccatga
     2581 tcttcgatga gatcgcgcag aataaagaca acctggcgca aatctcgatc aaatatcaga
     2641 atcagggcaa aaaagacctg ttacaagcct cggccgaaga cgacgtgaaa gccatcaagg
     2701 acctgttgga tcagacgaac aacttgttgc acaagctgaa aattttccac atcagccaga
     2761 gcgaggacaa ggccaacatc ttggacaaag acgagcactt ctacttggtg ttcgaagagt
     2821 gctacttcga attggccaac atcgtccccc tgtacaataa gattcgcaat tatattacgc
     2881 agaagcccta tagcgacgag aaattcaagt tgaattttga aaattcgacc ctggcgaatg
     2941 gctgggacaa gaacaaagag cccgacaaca ccgccatctt gttcatcaag gatgataaat
     3001 attatctggg ggtgatgaac aaaaagaata acaagatctt tgatgacaag gccatcaagg
     3061 agaataaggg cgaggggtac aagaaaatcg tctacaaact gttgcccggc gccaacaaga
     3121 tgttgccgaa ggtcttcttc agcgcgaaga gcattaaatt ctataacccc agcgaggaca
     3181 tcctgcgcat ccggaatcat tcgacccaca cgaagaatgg cagcccgcaa aagggctacg
     3241 agaagttcga gtttaacatc gaggattgtc ggaaattcat cgacttctat aaacagtcga
     3301 tcagcaagca ccccgagtgg aaggatttcg gcttccgctt cagcgatacc caacgctaca
     3361 acagcatcga cgagttttat cgggaagtgg aaaaccaggg gtacaagttg accttcgaga
     3421 acatttcgga gtcgtacatt gactcggtcg tcaaccaagg gaaactgtac ctgttccaaa
     3481 tctacaataa ggacttcagc gcgtactcga aagggcggcc caacttgcac accctgtatt
     3541 ggaaggcctt gtttgacgag cggaacttac aagacgtcgt ctataaactg aacggggagg
     3601 cggagctgtt ttaccgcaag cagtcgatcc cgaaaaagat tacccacccg gcgaaggagg
     3661 cgatcgccaa caaaaacaag gacaacccga agaaagagtc ggtcttcgaa tacgatctga
     3721 tcaaagacaa gcgctttacc gaggacaagt tctttttcca ttgtccgatc accatcaatt
     3781 ttaagagcag cggcgcgaac aagttcaacg acgagatcaa cctgctgttg aaagaaaagg
     3841 ccaatgatgt gcacatcctg tcgatcgacc ggggggaacg gcacctggcc tactacaccc
     3901 tggtggacgg caagggcaac atcatcaagc aggatacctt caatatcatt gggaacgacc
     3961 ggatgaaaac gaactaccac gacaagctgg cggccatcga aaaggatcgc gacagcgccc
     4021 gcaaggactg gaagaagatt aacaatatca aggagatgaa ggaggggtac ttgagccagg
     4081 tcgtgcacga gatcgccaag ctggtcattg agtacaacgc gattgtggtc tttgaagacc
     4141 tgaactttgg cttcaaacgg gggcggttca aggtcgagaa gcaggtgtat cagaagctgg
     4201 aaaaaatgtt gatcgagaag ctgaactatc tggtgttcaa agacaacgag tttgacaaaa
     4261 cggggggcgt cctgcgggcc taccagttga ccgccccctt cgaaaccttc aagaaaatgg
     4321 gcaagcagac gggcatcatc tactacgtcc cggcgggctt tacgagcaag atctgtccgg
     4381 tgaccggctt cgtcaaccag ctgtatccga agtatgagag cgtgtcgaaa agccaggagt
     4441 ttttctcgaa gttcgataaa atctgttata acctggataa ggggtacttc gaattttcgt
     4501 tcgattacaa aaacttcggg gacaaggcgg ccaaggggaa atggacgatc gccagcttcg
     4561 gctcgcgcct gattaacttt cgcaactcgg acaagaacca taactgggac acccgggaag
     4621 tgtacccgac caaagaattg gaaaaactgt tgaaggacta tagcatcgag tacgggcacg
     4681 gggagtgcat caaagccgcc atctgcgggg aaagcgacaa gaagtttttt gcgaagctga
     4741 ccagcgtgct gaatacgatc ttacaaatgc gtaattctaa gaccggcacc gagctggact
     4801 acctgatctc gccggtggcg gatgtcaacg gcaacttctt cgacagccgc caggccccga
     4861 agaacatgcc gcaggacgcg gatgcgaatg gcgcgtacca catcgggctg aaagggctga
     4921 tgctgctggg gcggatcaag aacaaccagg aggggaagaa gctgaatctg gtgattaaga
     4981 acgaggaata tttcgagttc gtccaaaatc gcaacaattg aataacttcg tataatgtat
     5041 gctatacgaa gttattaatt ctgcagagtc ctactagttc catgagcgtc agaccccgta
     5101 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5161 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5221 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     5281 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5341 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5401 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5461 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5521 agcgccacgc ttcccgaggg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5581 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5641 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5701 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5761 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     5821 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcaacgcgtg agcccaccag
     5881 ctccgtaagt tcgggcgctg tgtggctcgt acccgcgcat tcaggcggca gggggtctaa
     5941 cgggtctaag gcggcgtgta cggccgccac agcggctctc agcggcccgg aaacgtcctc
     6001 gaaacgacgc atgtgttcct cctggttggt acaggtggtt gggggtgctc ggctgtcgct
     6061 ggtgttccac caccagggct cgacgggaga gcgggggagt gtgcagttgt ggggtggccc
     6121 ctcagcgaaa tatctgactt ggagctcgtg tcggaccata caccggtgat taatcgtggt
     6181 ctactaccaa gcgtgagcca cgtcgccgac gaatttgagc agctctggct gccgtactgg
     6241 ccgctggcaa gcgacgatct gctcgagggg atctaccgcc aaagccgcgc gtcggcccta
     6301 ggccgccggt acatcgaggc gaacccaaca gcgctggcaa acctgctggt cgtggacgta
     6361 gaccatccag acgcagcgct ccgagcgctc agcgcccggg ggtcccatcc gctgcccaac
     6421 gcgatcgtgg gcaatcgcgc caacggccac gcacacgcag tgtgggcact caacgcccct
     6481 gttccacgca ccgaatacgc gcggcgtaag ccgctcgcat acatggcggc gtgcgccgaa
     6541 ggccttcggc gcgccgtcga tggcgaccgc agttactcag gcctcatgac caaaaacccc
     6601 ggccacatcg cctgggaaac ggaatggctc cactcagatc tctacacact cagccacatc
     6661 gaggccgagc tcggcgcgaa catgccaccg ccgcgctggc gtcagcagac cacgtacaaa
     6721 gcggctccga cgccgctagg gcggaattgc gcactgttcg attccgtcag gttgtgggcc
     6781 tatcgtcccg ccctcatgcg gatctacctg ccgacccgga acgtggacgg actcggccgc
     6841 gcgatctatg ccgagtgcca cgcgcgaaac gccgaatttc cgtgcaacga cgtgtgtccc
     6901 ggaccgctac cggacagcga ggtccgcgcc atcgccaaca gcatttggcg ttggatcaca
     6961 accaagtcgc gcatttgggc ggacgggatc gtggtctacg aggccacact cagtgcgcgc
     7021 cagtcggcca tctcgcggaa gggcgcagca gcgcgcacgg cggcgagcac agttgcgcgg
     7081 cgcgcaaagt ccgcgtcagc catggaggca ttgctatgag cgacggctac agcgacggct
     7141 acaaccggca gccgactgtc cgcaaaaagc ggcgcgtgac cgccgccgaa ggcgctcgaa
     7201 tcaccggact atccgaacgc cacgtcgtcc ggctcgtggc gcaggaacgc agcgagtggc
     7261 tcgccgagca ggctgcacgc cgcgaacgca tccgcgccta tcacgacgac gagggccact
     7321 cttggccgca aacggccaaa catttcgggc tgcatctgga caccgttaag cgactcggct
     7381 atcgggcgag gaaagagcgt gcggcagaac aggaagcggc tcaaaaggcc cacaacgaag
     7441 ccgacaatcc accgctgttc taacgcaatt ggggagcggg tgtcgcgggg gttccgtggg
     7501 gggttccgtt gcaacgggtc ggacaggtaa aagtcctggt agacgctagt tttctggttt
     7561 gggccatgcc tgtctcgttg cgtgtttcgt tgcgcccgtt ttgaatacca gccagacgag
     7621 acggggttct acgaatcttg gtcgatacca agccatttcc gctgaatatc ggggagctca
     7681 ccgccagaat cggtggttgt ggtgatgtac gtggcgaact ccgttgtagt gcctgtggtg
     7741 gcatccgtgg cgcggccgcg gtaccagatc tttaaatcta gataaagaag tgacgcggtc
     7801 tcaagcgtcg agcgtcgcca gcgtgtcgag gatgtcgaag tcgtagccgt cggcgctggc
     7861 gatgtagacc tgctggtcga attgactgtc gcgcatacac atcgggcccc ggggcccgtc
     7921 gaacccgaca tcgtgcgcgg atgccatcag gtccggtatc tcgggggagt gggcccgctg
     7981 gaagatggcc tcgagcgcaa gcagaccctc gtaacaggat tcggccatcg cgttgagcgg
     8041 tggcgcgtcg gcgccgtagc gggcgacgta gctgcccatc aggtccatgg cacccgcggt
     8101 ggccagtgaa ctgaagtacg ccgcggcgac atagaggttt tcggtggagc cggcgccgct
     8161 ggccagcagc atgttctcct ccatcagcgg gctgaaccgc gccatgcggt cgtgcccgcc
     8221 ggcgcgcgcg aactcgcggt tgaacaacac ggcgtcctgg ccgacgagca gcatcaacac
     8281 ggcctgcgcc cccgacgcga tggccttgcg gacaggtgcg cggaaatcgt cggtgccgta
     8341 cgggacgtag atctcccgtc tgagctcgag gtccagatct cggcagtacg cgcgggcggc
     8401 cgcggcggaa cggcgcggcc agatgtagtc atcgccgacc aggcaccagg accggatgcc
     8461 gaagtggtcg cgcagccagg cgagcgcggg cgcgatctgg atctgcggtg tctcgcctgt
     8521 gcagaacacg cccggtgtgc gttcaccgcc ctcgtacaac gaggtgtaga cgtacgggat
     8581 gcggtcgcgg accaccgggg agatgcggtt gcgcacggcc gagatgtgcc agccggtcac
     8641 ggcgtcgaga ccgtgacctc gcaaccggtc ggcgacggtc cgggcgacgt cgtcgccggg
     8701 cgctccgccg tcgagcacct cgatggtgac cttgcggccc tgcaggccgc ctcggtcgtt
     8761 gacctccttg gccgcgagct cggccacggc ctcgcacgaa ggcgcgaaga ttcccgctgg
     8821 cccttgaagc ggaatcacca gcccgacgcg gaactcaacc tcgccgtcct gcactccaga
     8881 tcaccgtcga tcccgtgtag tctgcgcttc aaagctttct agcagaaata attcattctg
     8941 aacagacccc gccgtcgaca cgaggagaca cccaccatgg ccgccggaca gcagcgccgc
     9001 cccaacctcc tgctgccgtt ggtgcgtctg acccacctcg cggagtcggc gatcgaacgc
     9061 gtgctcgcgg actcgtcgct caagatcgag gactggcgcg tgctcgacga gttggccgga
     9121 cggcgcaccg tgcccatgag cgatctcgcg caggccacgc tgatcacggg tccgactctc
     9181 accagaaccg tcgatcgcct tgtgtcgcaa gggatcatct accggactgc cgatctgcat
     9241 gaccgccggc gggtgctcgt ggcgttgacc ccgcgggggc ggacgctgcg caaccgcctg
     9301 gtggacgcgg tagccgaggc cgagtgtgcg gcttttgaat cgtgcgggct ggacgtcgac
     9361 cagttgcgcg aactcgtcga caccacctcg aatttgactt cgtaaccacc cgcgcccggc
     9421 gcgggcgttc acccttgact tttattttca tctggatata tttcgggtga atggaaaggg
     9481 gtgaccatgc cgacctacac attccgttgt tcccactgcg gtcccttcga tctcacctgc
     9541 gcgatctccg agcgcgatgc ggcggcgacc tgtccggagt gccggacgcc ggcgcgccgg
     9601 gtcttcggtt cggtagggct gacgacattc accgcgggac atcaccgcgc attcgacgcg
     9661 gcgtccgcga gcgccgaaag tcccacggtg gtgaagtcga ttcccgcagg cgcggaccgc
     9721 ccgcgggccc cgcgccgcaa tcccggtcta ccgagtctgc cgaggtacta gcgacatggg
     9781 tggcgtcggg ctcttctacg tgggtgcggt gctcatcatc gacgggctga tgctgctggg
     9841 ccgcatcagc ccacgaggcg caacaccgct gaacttcttc gtcggcggac tgcaggtggt
     9901 gacgcctacg gtgctgatcc tgcagtccgg cggagacgcg gccgtgatct tcgcggcctc
     9961 cgggctctac ctgttcggct tcacctacct gtgggtggcc atcaacaacg tgaccgactg
    10021 ggacggagaa ggtctcggat ggttctcgct gttcgtcgcg atcgccgcac tcggctactc
    10081 gtggcacgcg ttcaccgccg aggccgaccc ggcgttcggg gtgatctggc tgctgtgggc
    10141 agtgctgtgg ttcatgctgt tcctgctgct cggcctgggg cacgacgcac tggggcccgc
    10201 cgtcgggttc gtcgcggtgg ccgaaggcgt gatcaccgcc gccgtgccgg ccttcctgat
    10261 cgtgtcgggc aactgggaaa ccggcccgct ccccgccgcg gtcatcgccg tgatcggttt
    10321 tgccgcagtt gttctcgcat accccatcgg gcgccgtctc gcagcgccgt cagtcaccaa
    10381 ccctccaccg gccgcgctcg cggccaccac ccgataagag aaagggagtc cacatatgta
    10441 acggatccag ctgcagaatt cgaagcttat cgatgtcgac gtagttacga gatcggcggc
    10501 cgcatatgag tgtgcccaca caggacggaa tgcaccggtt cgtcgacgag gacgtctacc
    10561 acgctgaccg gggctcgctg tcggtatccg gcgcgaagct gctgttgccg ccgtcgtgtc
    10621 ccgcgaaatt ccgctgggag atggacaaca cccggaagcc gaaaaaggtc tgggacttcg
    10681 gacatgtcgc gcacaaactg gtgctcggca agggtgccga gttcgagatc ctcgaccccg
    10741 aggtgcacgg gctgaaggcg gacggtacgc cgtcggagaa gccgaccgcg acgggcatgt
    10801 ggcgcaaggc cgaggctgag gctcgcaaac agggcaaggt gccgattcac gtcgacctgt
    10861 tcacgaaggc gtacgacatg gccgaaaagg tgcgtcagca cccgacagcc ggcccgatct
    10921 tcgccaatcc tgacggcgag gccgaggtcg cgctgtacta caccgacccc gagaccggcg
    10981 tgcggctgcg tggccggatc gactggctca ctgacgatat cgatgattac aagacgtcga
    11041 tgaccgcgaa cccggccgag ctgaaaacca agttctacaa gctcggctat ttcatgcagg
    11101 cggcctggta catcgatcta ctggtcgccc tcgggctcgc cgagaacccg cgattccggt
    11161 tcatcacgca ggagaaagaa ccgccctacg tcgtgactcc gatccagtac gacgacgagg
    11221 cgatcgaaga ggggcggcgc cgcaaccgcc aggcgatccg gctctacgcc gactgcatgg
    11281 aatcgggcaa gtggcctgac tacagcgacg acgtggtcac gatcagcctg ccctcgtggg
    11341 ggctgccgcg accgcagacc gtcggcgacg tcgtcaccga cagctatatc tacgacaccg
    11401 acccgctcga agaggccgac ccgattgaag gggattacat ctatggctga aaatgctgtc
    11461 accaagcagg attcgcccaa ggcacccgag acgatctcgc aagtgctgca ggtgctcgtg
    11521 ccgcagctgg cgcgtgcagt gcccaagggt atggaccccg accgcatcgc gcggatcgtg
    11581 caaaccgaga tccgcaagtc gcgcaacgcg aaagctgctg gcatcgctaa gcagtccctc
    11641 gacgactgca cgcaagagtc atttgccggt gcgctgctga cctcggccgc gctcggtctc
    11701 gagcccggtg tcaacggcga gtgctacctc gtgccctacc gcgacacccg gcgcggcgtg
    11761 gtcgagtgcc agctgatcat cggctaccag ggcatcgtca aactgttctg gcagcacccg
    11821 cgcgcctccc ggatcgacgc gcagtgggtc ggcgcgaacg acgaattcca ttacaccatg
    11881 ggcctcaatc cgacgctgaa acacgtgaag gccaagggtg atcggggtaa tccggtctac
    11941 ttctacgcga tcgtcgaggt gaccggcgct gagccgctgt gggacgtgtt caccgccgac
    12001 gagatcaggg aattgcgtcg cggcaaggtc ggatcctcgg gcgacatcaa ggacccgcag
    12061 cgctggatgg agcggaagac cgcgctcaaa caggtgctga agctggcacc gaagacgacg
    12121 cggctcgacg cggcgatccg cgccgacgat cgcccgggca ccgacctgtc acagtcgcag
    12181 gcgctcgcgc tgccgtcgac cgtcaagccg acggccgact acatcgacgg cgagatcgcc
    12241 gagccgcacg aggtcgatac gccaccgaag tcgtcgcgcg cacaacgcgc gcagcgcgcc
    12301 accgcgccgg cgcccgacgt gcagatggcc aatcccgatc agctgaagcg cctcggcgag
    12361 atccagaagg ccgaaaagta caacgacgcc gattggttca agttcctcgc cgattcggcc
    12421 ggcgtcaaag ccacgcgcgc cgccgacctc acgttcgacg aggcgaaggc tgtcatcgac
    12481 atgttcgacg ggcccaacgc atgagcgccc cggcgaattc cgacgcggtg gttgatctgc
    12541 aactagcgta cgatcgactg ccaggcatca aataaaacga aaggctcagt cgaaagactg
    12601 ggcctttcgt tttatgccat catggccgcg gtgatcagct agccacctga cgtcgggggg
    12661 gggggaaagc cacgttgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
    12721 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
    12781 ccatattcaa cgggaaacgt cttgctcgag gccgcgatta aattccaaca tggatgctga
    12841 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
    12901 cttgtatggg aagccccatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
    12961 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
    13021 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
    13081 cgggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
    13141 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
    13201 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
    13261 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
    13321 gcataatctt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
    13381 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
    13441 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
    13501 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
    13561 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg
    13621 gcagagcatt acgctgactt gacgggacgg cggctttgtt gaataaatcg aacttttgct
    13681 gagttgaagg atcagatcac gcatcttccc gacaacgcag accgttccgt ggcaaagcaa
    13741 aagttcaaaa tcaccaactg gtccacctac aacaaagctc tcaccaaccg tggctccctc
    13801 actttctggc tggatgatgg ggcgattcag gcctggtatg agtcagcaac accttcttca
    13861 cgaggcagac ctcactagtg gccatgatgg cataaaacga aaggcccagt ctttcgactg
    13921 agcctttcgt tttatttgat gcctggcagt cgatcgtacg ctagttaact acgtcgacat
    13981 cgataagctt ggcgtgaatt c
//
