LOCUS       sequence-320866-        2127 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 N-terminal tag  part for MoClo assembly, CCAT-AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Dudley QM, Cai YM, Kallam K, Debreyne H, Carrasco Lopez JA, Patron 
            NJ
  TITLE     Biofoundry-assisted expression and characterization of plant 
            proteins.
  JOURNAL   Synth Biol (Oxf). 2021 Sep 11;6(1):ysab029. doi: 
            10.1093/synbio/ysab029. eCollection 2021.
  PUBMED    34693026
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-09-11"; volume: "6(1):ysab029. doi"; pages: " 
            10.1093/synbio/ysab029. eCollection 2021"
COMMENT     Sequence Label: pEPQD0CM0281
FEATURES             Location/Qualifiers
     source          1..2127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             59..103
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             104..124
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     terminator      141..198
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(222..239)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(393..981)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(473..492)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1164..1258)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1279..1938)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1853..1872
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2100..2118
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(join(2122..2127,1..38))
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcggtc tcaccatgaa
       61 agaaacagca gccgcgaaat tcgaacgtca gcatatggat tcagagaacc tgtattttca
      121 gtcaatgcga gaccctgcag tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt
      181 tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg
      241 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      301 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
      361 aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag
      421 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
      481 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
      541 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
      601 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
      661 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
      721 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
      781 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
      841 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
      901 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
      961 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1021 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1081 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1141 tggtctgaca gctcgaggct tggattctca ccaataaaaa acgcccggcg gcaaccgagc
     1201 gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca
     1261 agcgagctcg atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc
     1321 attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag
     1381 cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa
     1441 gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc
     1501 tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta
     1561 acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact
     1621 ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact
     1681 atcccatatc accagctcac cgtctttcat tgccatacga aattccggat gagcattcat
     1741 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt
     1801 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga
     1861 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc
     1921 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa
     1981 tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat
     2041 caactcgagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     2101 ataggcgtat cacgaggcag aatttca
//
