LOCUS       sequence-320880-        2427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 N-terminal tag  part for MoClo assembly, CCAT-AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Dudley QM, Cai YM, Kallam K, Debreyne H, Carrasco Lopez JA, Patron 
            NJ
  TITLE     Biofoundry-assisted expression and characterization of plant 
            proteins.
  JOURNAL   Synth Biol (Oxf). 2021 Sep 11;6(1):ysab029. doi: 
            10.1093/synbio/ysab029. eCollection 2021.
  PUBMED    34693026
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-09-11"; volume: "6(1):ysab029. doi"; pages: " 
            10.1093/synbio/ysab029. eCollection 2021"
COMMENT     Sequence Label: pEPQD0CM0548
FEATURES             Location/Qualifiers
     source          1..2427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             380..400
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(468..484)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(468..484)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(481..503)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(656..1244)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(736..755)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1377..1471)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1492..2151)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2066..2085
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2152..2254)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2341..2363
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2355..2372
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2356..2372
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tctcaccatg gtaagcggct ggcgtctgtt caagaaaatt agcggaagtg ataagattat
       61 tcatttaaca gatgacagct ttgatacaga cgtgctgaag gcagatggag caattctggt
      121 agacttttgg gcggaatggt gtggcccatg taagatgatt gcgccgatct tagacgagat
      181 tgcagatgag taccaaggta agttgacggt ggcgaagctc aatattgacc agaatccggg
      241 taccgcacct aagtacggaa ttcgcggaat tccaaccttg ttactcttta agaatggcga
      301 ggttgccgcc acgaaggtcg gcgcgttaag caagggccaa ctgaaggaat tcttggatgc
      361 gaatttagca ggcagtagcg agaacctgta ttttcagtca atgagagacc taggctaggt
      421 ggaggctcag tgatgataag tctgcgatgg tggatgcatg tgtcatggtc atagctgttt
      481 cctgtgtgaa attgttatcc gctcagaggg cacaatccta ttccgcgcta tccgacaatc
      541 tccaagacat taggtggagt tcagttcggc gtatggcata tgtcgctgga aagaacatgt
      601 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      661 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      721 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      781 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      841 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      901 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
      961 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     1021 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     1081 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     1141 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     1201 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     1261 tttctacggg gtctgacgct ctattcaaca aagccgccgt cccgtcaagt cagcgtaaat
     1321 gggtaggggg cttcaaatcg tccccccata cgatataagt tgttactagt gcttggattc
     1381 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
     1441 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc
     1501 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
     1561 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
     1621 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
     1681 atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa
     1741 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
     1801 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
     1861 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
     1921 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
     1981 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
     2041 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
     2101 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
     2161 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
     2221 atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattgatacc tgaaacaaaa
     2281 cccatcgtac ggccaaggaa gtctccaata actgtgatcc accacaagcg ccagggtttt
     2341 cccagtcacg acgttgtaaa acgacggcca gtcatgcata atccgcacgc atctggaata
     2401 aggaagtgcc attccgcctg acctagg
//
