LOCUS       sequence-320881-        2097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 C-terminal tag  part for MoClo assembly, TTCG-GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Dudley QM, Cai YM, Kallam K, Debreyne H, Carrasco Lopez JA, Patron 
            NJ
  TITLE     Biofoundry-assisted expression and characterization of plant 
            proteins.
  JOURNAL   Synth Biol (Oxf). 2021 Sep 11;6(1):ysab029. doi: 
            10.1093/synbio/ysab029. eCollection 2021.
  PUBMED    34693026
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-09-11"; volume: "6(1):ysab029. doi"; pages: " 
            10.1093/synbio/ysab029. eCollection 2021"
COMMENT     Sequence Label: pEPYC0CM0134
FEATURES             Location/Qualifiers
     source          1..2097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     107..126
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     354..372
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(376..419)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      492..549
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(573..590)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(744..1332)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(824..843)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1515..1609)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1630..2097,1..192))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
       61 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
      121 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
      181 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
      241 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaactc
      301 gagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
      361 gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct
      421 ggaattcggt ctcattcggt gagcggctgg cggctgttca agaagattag ctgagcttcg
      481 agaccctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa
      541 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
      601 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      661 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      721 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
      781 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      841 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      901 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      961 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1021 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1081 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1141 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1201 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1261 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1321 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1381 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1441 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1501 agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     1561 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
     1621 gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
     1681 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
     1741 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     1801 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
     1861 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
     1921 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
     1981 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
     2041 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcg
//
