LOCUS       sequence-320883-        2828 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 C-terminal tag  part for MoClo assembly, TTCG-GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Dudley QM, Cai YM, Kallam K, Debreyne H, Carrasco Lopez JA, Patron 
            NJ
  TITLE     Biofoundry-assisted expression and characterization of plant 
            proteins.
  JOURNAL   Synth Biol (Oxf). 2021 Sep 11;6(1):ysab029. doi: 
            10.1093/synbio/ysab029. eCollection 2021.
  PUBMED    34693026
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-09-11"; volume: "6(1):ysab029. doi"; pages: " 
            10.1093/synbio/ysab029. eCollection 2021"
COMMENT     Sequence Label: pEPQD0CM0027
FEATURES             Location/Qualifiers
     source          1..2828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     123..141
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(145..188)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             874..921
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             922..945
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     terminator      992..1049
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1073..1090)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1244..1832)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1324..1343)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2015..2109)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2130..2789)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2704..2723
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc
       61 gatcaactcg agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
      121 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
      181 atgatttctg gaattcggtc tcattcgggc gggagcggtg gaagcggcag caaaggtgaa
      241 gaactgttta ccggcgttgt gccgattctg gtggaactgg atggcgatgt gaacggtcac
      301 aaattcagcg tgcgtggtga aggtgaaggc gatgccacga ttggcaaact gacgctgaaa
      361 tttatctgca ccaccggcaa actgccggtg ccgtggccga cgctggtgac caccctgacc
      421 tatggcgttc agtgttttag tcgctatccg gatcacatga aacgtcacga tttctttaaa
      481 tctgcaatgc cggaaggcta tgtgcaggaa cgtacgatta gctttaaaga tgatggcaaa
      541 tataaaacgc gcgccgttgt gaaatttgaa ggcgataccc tggtgaaccg cattgaactg
      601 aaaggcacgg attttaaaga agatggcaat atcctgggcc ataaactgga atacaacttt
      661 aatagccata atgtttatat tacggcggat aaacagaaaa atggcatcaa agcgaatttt
      721 accgttcgcc ataacgttga agatggcagt gtgcagctgg cagatcatta tcagcagaat
      781 accccgattg gtgatggtcc ggtgctgctg ccggataatc attatctgag cacgcagacc
      841 gttctgtcta aagatccgaa cgaaaaaggc acgcgggacc acatggttct gcacgaatat
      901 gtgaatgcgg caggtattac gtggagccat ccgcagttcg aaaaataagt cgaccggctg
      961 ctaacaaagc ccgagcttcg agaccctgca gtccggcaaa aaagggcaag gtgtcaccac
     1021 cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca
     1081 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1141 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1201 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc
     1261 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1321 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1381 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1441 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1501 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1561 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1621 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1681 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1741 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1801 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1861 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     1921 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     1981 atgagtaaac ttggtctgac agctcgaggc ttggattctc accaataaaa aacgcccggc
     2041 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
     2101 acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac
     2161 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
     2221 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
     2281 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     2341 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     2401 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     2461 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     2521 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga
     2581 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     2641 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     2701 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     2761 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     2821 aactcaaa
//
