LOCUS       sequence-320884-        9655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of Flag-Polß(WT) protein with hygromycin 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9655)
  AUTHORS   Fang Q, Inanc B, Schamus S, Wang XH, Wei L, Brown AR, Svilar D, 
            Sugrue KF, Goellner EM, Zeng X, Yates NA, Lan L, Vens C, Sobol RW
  TITLE     HSP90 regulates DNA repair via the interaction between XRCC1 and DNA
            polymerase beta.
  JOURNAL   Nat Commun. 2014 Nov 26;5:5513. doi: 10.1038/ncomms6513.
  PUBMED    25423885
REFERENCE   2  (bases 1 to 9655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-11-26"; volume: "5:5513. doi"; pages: " 
            10.1038/ncomms6513"
COMMENT     Sequence Label: pLVX-Flag-Polß(WT)--IRES-hyg
FEATURES             Location/Qualifiers
     source          1..9655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(165..182)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     392..411
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             610..1629
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             610..1629
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    1643..2231
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1696..1716)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2114..2125)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2438..3071
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3199..3215)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3199..3215)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3212..3234)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3223..3239
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3247..3253,3254..3271,3272..3277))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3292..3313
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3430..3447)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3601..4189)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3681..3700)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4360..5151,5152..5220))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4983..5002
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5221..5325)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    5373..5507
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5410..5429)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5464..5483
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5675..6308
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6355..6480
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6977..7210
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7395..7439
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7702..7817
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        7875..8178
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8179..8382
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8333..8353
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8379..8403
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    8498..8522
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8546..8569
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(9597..9621)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(9653..9655,1..572,573..575,576..584)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
       61 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
      121 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
      181 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
      241 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
      301 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
      361 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
      421 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
      481 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
      541 cgtggttttc ctttgaaaaa cacgatgata agcttgccac aacccgtacc aaagatggat
      601 agatccggaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
      661 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
      721 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
      781 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
      841 attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
      901 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
      961 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
     1021 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
     1081 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
     1141 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
     1201 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
     1261 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
     1321 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
     1381 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
     1441 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
     1501 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
     1561 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
     1621 aaggaataga cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac
     1681 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     1741 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     1801 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     1861 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     1921 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     1981 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     2041 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     2101 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     2161 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     2221 cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca
     2281 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     2341 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     2401 gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa
     2461 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
     2521 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
     2581 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
     2641 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
     2701 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
     2761 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
     2821 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
     2881 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     2941 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     3001 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     3061 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     3121 atgaatatca gagagtgaga ggccttgaca ttgctagcgt ttaccgtcga cctctagcta
     3181 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     3241 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     3301 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     3361 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     3421 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3481 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3541 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3601 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3661 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3721 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3781 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3841 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3901 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3961 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4021 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4081 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4141 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4201 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4261 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4321 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     4381 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4441 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4501 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     4561 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4621 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     4681 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     4741 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4801 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     4861 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4921 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     4981 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     5041 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     5101 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     5161 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     5221 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     5281 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     5341 agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt
     5401 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5461 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc
     5521 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
     5581 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
     5641 gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga
     5701 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag
     5761 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
     5821 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta
     5881 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt
     5941 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac
     6001 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc
     6061 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
     6121 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6181 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     6241 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     6301 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     6361 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     6421 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     6481 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     6541 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     6601 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     6661 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     6721 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     6781 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac
     6841 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     6901 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     6961 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     7021 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     7081 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     7141 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     7201 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     7261 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     7321 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     7381 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     7441 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     7501 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     7561 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     7621 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     7681 acggatctcg acggtatcgc ctttaaaaga aaagggggga ttggggggta cagtgcaggg
     7741 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     7801 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttatcgataa
     7861 gcttgggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     7921 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     7981 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     8041 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     8101 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     8161 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     8221 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     8281 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     8341 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
     8401 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg actctactag
     8461 aggatctatt tccggtgaat tcctcgagac tagatcaaca agtttgtaca aaaaagcagg
     8521 ctccgcggcc gcccccttca ccatggacta caaagacgat gacgataaag gcatgagcaa
     8581 acggaaggcg ccgcaggaga ctctcaacgg gggaatcacc gacatgctca cagaactcgc
     8641 aaactttgag aagaacgtga gccaagctat ccacaagtac aatgcttaca gaaaagcagc
     8701 atctgttata gcaaaatacc cacacaaaat aaagagtgga gctgaagcta agaaattgcc
     8761 tggagtagga acaaaaattg ctgaaaagat tgatgagttt ttagcaactg gaaaattacg
     8821 taaactggaa aagattcggc aggatgatac gagttcatcc atcaatttcc tgactcgagt
     8881 tagtggcatt ggtccatctg ctgcaaggaa gtttgtagat gaaggaatta aaacactaga
     8941 agatctcaga aaaaatgaag ataaattgaa ccatcatcag cgaattgggc tgaaatattt
     9001 tggggacttt gaaaaaagaa ttcctcgtga agagatgtta caaatgcaag atattgtact
     9061 aaatgaagtt aaaaaagtgg attctgaata cattgctaca gtctgtggca gtttcagaag
     9121 aggtgcagag tccagtggtg acatggatgt tctcctgacc catcccagct tcacttcaga
     9181 atcaaccaaa cagccaaaac tgttacatca ggttgtggag cagttacaaa aggttcattt
     9241 tatcacagat accctgtcaa agggtgagac aaagttcatg ggtgtttgcc agcttcccag
     9301 taaaaatgat gaaaaagaat atccacacag aagaattgat atcaggttga tacccaaaga
     9361 tcagtattac tgtggtgttc tctatttcac tgggagtgat attttcaata agaatatgag
     9421 ggctcatgcc ctagaaaagg gtttcacaat caatgagtac accatccgtc ccttgggagt
     9481 cactggagtt gcaggagaac ccctgccagt ggatagtgaa aaagacatct ttgattacat
     9541 ccagtggaaa taccgggaac ccaaggaccg gagcgaatga aagggtgggc gcgccgaccc
     9601 agctttcttg tacaaagtgg ttgatctagt tctagagcgg ccgcggatcc cgccc
//
