LOCUS       sequence-320889-        2096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 C-terminal tag  part for MoClo assembly, TTCG-GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Dudley QM, Cai YM, Kallam K, Debreyne H, Carrasco Lopez JA, Patron 
            NJ
  TITLE     Biofoundry-assisted expression and characterization of plant 
            proteins.
  JOURNAL   Synth Biol (Oxf). 2021 Sep 11;6(1):ysab029. doi: 
            10.1093/synbio/ysab029. eCollection 2021.
  PUBMED    34693026
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-09-11"; volume: "6(1):ysab029. doi"; pages: " 
            10.1093/synbio/ysab029. eCollection 2021"
COMMENT     Sequence Label: pEPYC0CM0257
FEATURES             Location/Qualifiers
     source          1..2096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     160..179
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     407..425
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(429..472)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      544..601
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(625..642)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(796..1384)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(876..895)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1567..1661)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1682..2096,1..245))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
       61 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
      121 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
      181 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
      241 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
      301 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc
      361 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
      421 gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc
      481 ggtctcattc gtgagcggct ggcggctgtt caagaagatt agctgagctt cgagaccctg
      541 cagtccggca aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
      601 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      661 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      721 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      781 cgcgttgctg gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg
      841 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      901 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      961 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     1021 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1081 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1141 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1201 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1261 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1321 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1381 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     1441 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     1501 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag
     1561 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     1621 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa
     1681 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     1741 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     1801 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     1861 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     1921 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     1981 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     2041 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcacc
//
