LOCUS       sequence-320890-        6122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Blue-light dependent heterodimerization system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Benedetti L, Marvin JS, Falahati H, Guillen-Samander A, Looger LL, 
            De Camilli P
  TITLE     Optimized Vivid-derived Magnets photodimerizers for subcellular 
            optogenetics in mammalian cells.
  JOURNAL   Elife. 2020 Nov 11;9. pii: 63230. doi: 10.7554/eLife.63230.
  PUBMED    33174843
REFERENCE   2  (bases 1 to 6122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-11-11"; volume: "9. pii: 63230. doi"; pages: " 
            10.7554/eLife.63230"
COMMENT     Sequence Label: mCherry- eMagBF - 5ptaseOCRL
FEATURES             Location/Qualifiers
     source          1..6122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        143..446
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        447..650
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     601..621
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      688..697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             694..1401
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(839..857)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1107..1126
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    2992..3113
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3029..3048)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3083..3102
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3120..3575)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3257..3278)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3469..3488
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3602..3706
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3708..4065
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3708..3899
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3731..3751)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3916..4051
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4100..4894
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4100..4894
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4154..4173)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4764..4783
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5082..5101)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5126..5173
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5502..6090
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5991..6010
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
       61 taaccgtatt accgccatgc attagttatt aatagtaatc aattacgggg tcattagttc
      121 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      181 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      241 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      301 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      361 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      421 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      481 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      541 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      601 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga
      661 accgtcagat ccgctagcgc taccggtgcc accatggtga gcaagggcga ggaggataac
      721 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      781 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      841 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      901 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
      961 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     1021 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     1081 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     1141 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     1201 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     1261 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     1321 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     1381 ggcatggacg agctgtacaa gagatctcga agcgcggccg cgatgggaca taccctctac
     1441 gcgccggggg gttatgacat catgggttac ctcagacaga tcagaaaccg gccgaaccca
     1501 caagtggagc tgggacccgt cgacctctcc tgcgccctcg tgctgtgtga ccttaagcag
     1561 aaggacaccc ctgtggtgta cgcctccgaa gcattcctgg agatgaccgg gtacaacaga
     1621 cacgaagtgc tgggacggaa ctgccgcttc ctgcaatccc cggatggaat ggtgaagcct
     1681 aagtcaaccc gcaaatacgt ggactccaac actatcttca ccatgaagaa ggccattgac
     1741 cgcaatgctg aggtgcaagt ggaagtggtg aacttcaaga agaacggaca gcgcttcgtc
     1801 aacttcctga ctatgattcc cgtgcgggac gaaaccggcg aataccggta cagcatcggg
     1861 tttcagtgcg agactgaggg ctccggcggc agcggtggct ccggaggcag cggaggtacc
     1921 cgatcgtatg tcaacattca gactttcaga ttttttgttg gaacttggaa tgtgaatggc
     1981 cagtctccag atagcgggtt agaaccttgg ctgaactgtg atcccaatcc tcctgatatc
     2041 tactgcattg gattccaaga actggacttg agcacagaag ccttcttcta ctttgaatct
     2101 gtgaaggaac aagaatggtc catggctgta gagagaggtt tgcattccaa agccaagtat
     2161 aagaaagttc aactggtgcg ccttgttggg atgatgcttc ttatatttgc cagaaaggat
     2221 cagtgtcgat acattcgtga tattgctaca gaaacagttg gaactggaat catggggaaa
     2281 atgggaaaca aaggtggggt agctgtgaga tttgtatttc acaacaccac cttttgcatt
     2341 gtcaattccc atctggctgc acacgtggag gactttgaga gaaggaatca agattataag
     2401 gacatttgtg cgagaatgag ttttgtggtc ccaaatcaga ccctcccgca gttgaacatc
     2461 atgaaacatg aggttgtcat ttggttggga gatttgaatt atagactttg catgcctgat
     2521 gccaatgagg tgaaaagtct tattaataag aaagaccttc agagactctt gaaattcgac
     2581 cagctaaata ttcagcgcac acagaaaaaa gcttttgttg acttcaatga aggggaaatc
     2641 aagttcatcc ccacttataa gtatgactct aaaacagacc ggtgggattc cagtgggaaa
     2701 tgccgggttc cagcctggtg tgaccgaatt ctttggagag gaacaaatgt taatcagctt
     2761 aattatcgga gtcacatgga actgaaaacc agcgaccaca agcctgttag cgccctcttc
     2821 catattgggg tgaaggttgt ggattgaggt accgcgggcc cgggatccac cggatctaga
     2881 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     2941 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     3001 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     3061 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta
     3121 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     3181 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     3241 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     3301 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     3361 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     3421 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     3481 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     3541 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
     3601 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3661 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag
     3721 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     3781 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     3841 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     3901 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3961 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     4021 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac
     4081 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     4141 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     4201 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     4261 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
     4321 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     4381 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     4441 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     4501 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     4561 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     4621 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     4681 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     4741 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     4801 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     4861 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     4921 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     4981 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     5041 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca
     5101 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt
     5161 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
     5221 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
     5281 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc
     5341 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5401 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5461 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5521 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5581 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5641 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5701 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5761 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5821 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5881 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5941 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6001 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6061 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6121 ct
//
