LOCUS       sequence-320896-        2090 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 C-terminal tag  part for MoClo assembly, TTCG-GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Dudley QM, Cai YM, Kallam K, Debreyne H, Carrasco Lopez JA, Patron 
            NJ
  TITLE     Biofoundry-assisted expression and characterization of plant 
            proteins.
  JOURNAL   Synth Biol (Oxf). 2021 Sep 11;6(1):ysab029. doi: 
            10.1093/synbio/ysab029. eCollection 2021.
  PUBMED    34693026
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-09-11"; volume: "6(1):ysab029. doi"; pages: " 
            10.1093/synbio/ysab029. eCollection 2021"
COMMENT     Sequence Label: pEPQD0CM0030
FEATURES             Location/Qualifiers
     source          1..2090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     102..121
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     349..367
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(371..414)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      480..537
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(561..578)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(732..1320)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(812..831)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1503..1597)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1618..2090,1..187))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
       61 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
      121 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
      181 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
      241 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactcgagtg
      301 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
      361 acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat
      421 tcggtctcat tcgtaagtcg accggctgct aacaaagccc gagcttcgag accctgcagt
      481 ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta
      541 cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      601 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      661 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      721 ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      781 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      841 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      901 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
      961 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1021 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1081 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1141 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     1201 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1261 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1321 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1381 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     1441 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt
     1501 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
     1561 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
     1621 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
     1681 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     1741 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     1801 gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc
     1861 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     1921 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
     1981 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     2041 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa
//
