LOCUS       sequence-320899-        7939 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Blue-light dependent heterodimerization system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Benedetti L, Marvin JS, Falahati H, Guillen-Samander A, Looger LL, 
            De Camilli P
  TITLE     Optimized Vivid-derived Magnets photodimerizers for subcellular 
            optogenetics in mammalian cells.
  JOURNAL   Elife. 2020 Nov 11;9. pii: 63230. doi: 10.7554/eLife.63230.
  PUBMED    33174843
REFERENCE   2  (bases 1 to 7939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-11-11"; volume: "9. pii: 63230. doi"; pages: " 
            10.7554/eLife.63230"
COMMENT     Sequence Label: TagRFP-T-VAPB(1- 218)- eMagB
FEATURES             Location/Qualifiers
     source          1..7939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          785..1801
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1725..1747
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      1903..1912
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1909..2640
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     intron          3831..3963
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3946..3965
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    3972..4560
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4025..4045)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4443..4454)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4587..4604)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4593..4704
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4800..4820)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4800..4818)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4839..4855)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4957..4976)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5022..5077
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5076..5095)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5438..5454)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5462..5478
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5486..5492,5493..5510,5511..5516))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5531..5552
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5611..5806
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5657..5792
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5812..5946
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5849..5868)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5903..5922
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6014..6031)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6185..6773)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6265..6284)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6944..7735,7736..7804))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7567..7586
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7805..7909)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg ggggggngsc ngcggggggg gggggggggg gggggggggg
      541 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggggggr
      601 gggggggggg gggggggggg gggggggggg ggngnggggg gggggggggg gggggggggg
      661 ggggggsggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
      721 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      781 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
      841 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
      901 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
      961 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
     1021 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
     1081 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     1141 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     1201 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     1261 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     1321 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     1381 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc
     1441 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     1501 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     1561 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     1621 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     1681 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     1741 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     1801 gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat tttggcaaag aattcggtcc
     1861 cgcgggcccg gcatccaccg gaagccacct gcatgcggcc gcgccaccat ggtgtctaag
     1921 ggcgaagagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac
     1981 aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     2041 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc
     2101 agcttcatgt acggcagcag aaccttcatc aaccacaccc agggcatccc cgacttcttt
     2161 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     2221 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     2281 atcagagggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg
     2341 gaggccaaca ccgagatgct gtaccccgct gacggcggcc tggaaggcag aaccgacatg
     2401 gccctgaagc tcgtgggcgg gggccacctg atctgcaact tcaagaccac atacagatcc
     2461 aagaaacccg ctaagaacct caagatgccc ggcgtctact atgtggacca cagactggaa
     2521 agaatcaagg aggccgacaa agagacctac gtcgagcagc acgaggtggc tgtggccaga
     2581 tactgcgacc tccctagcaa actggggcac aaacttaatg gcatggacga gctgtacaag
     2641 ggtacccgag ctcaagcttc gaattcgatg gcgaaggtgg agcaggtcct gagtctcgag
     2701 ccgcagcacg agctcaaatt ccgaggtccc ttcaccgatg ttgtcaccac caacctaaag
     2761 cttggcaacc cgacagaccg aaatgtgtgt tttaaggtga agactacagc accacgtagg
     2821 tactgtgtga ggcccaacag cggaatcatc gatgcagggg cctcaattaa tgtatctgtg
     2881 atgttacagc ctttcgatta tgatcccaat gagaaaagta aacacaagtt tatggttcag
     2941 tctatgtttg ctccaactga cacttcagat atggaagcag tatggaagga ggcaaaaccg
     3001 gaagacctta tggattcaaa acttagatgt gtgtttgaat tgccagcaga gaatgataaa
     3061 ccacatgatg tagaaataaa taaaattata tccacaactg catcaaagac agaaacacca
     3121 atagtgtcta agtctctgag ttcttctttg gatgacaccg aagttaagaa ggttatggaa
     3181 gaatgtaaga ggctgcaagg tgaagttcag aggctacggg aggagaacaa gcagttcaag
     3241 gaagaagatg gactgcggat gaggaagaca gtgcagagca acagccccat ttcagcatta
     3301 gccccaactg ggaaggaaga gatatcgatg ggacataccc tctacgcgcc ggggggttat
     3361 gacatcatgg gttacctcag acagatcaga aaccggccga acccacaagt ggagctggga
     3421 cccgtcgacc tctcctgcgc cctcgtgctg tgtgacctta agcagaagga cacccctgtg
     3481 gtgtacgcct ccgaagcatt cctggagatg accgggtaca acagacacga agtgctggga
     3541 cggaactgcc gcttcctgca atccccggat ggaatggtga agcctaagtc aacccgcaaa
     3601 tacgtggact ccaacactat cttcaccatg aagaaggcca ttgaccgcaa tgcagaggtg
     3661 caagtggaag tggtgaactt caagaagaac ggacagcgct tcgtcaactt cctgactatg
     3721 attcccgtgc gggacgaaac cggcgaatac cggtacagca tcgggtttca gtgcgagact
     3781 gagtaagtta acctcgaggt cccgatgaat agctaaggtc gaggccgcag gtaagtatca
     3841 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac
     3901 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca
     3961 caggtgtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     4021 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     4081 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     4141 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     4201 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     4261 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     4321 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     4381 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     4441 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     4501 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     4561 ctggaattcg agctcggtac gatcagcctc gactgtgcct tctagttgcc agccatctgt
     4621 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     4681 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     4741 tggggtgggg caggacagca agggggagga ttgggaagac aatagcccag cttttgttcc
     4801 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     4861 aattgttatc cgctaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
     4921 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
     4981 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
     5041 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     5101 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat
     5161 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc
     5221 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
     5281 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
     5341 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag
     5401 atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     5461 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     5521 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     5581 agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc
     5641 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     5701 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     5761 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt
     5821 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     5881 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     5941 tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6001 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6061 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6121 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6181 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6241 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6301 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6361 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     6421 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6481 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6541 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6601 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     6661 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6721 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6781 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6841 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     6901 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     6961 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7021 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7081 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7141 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7201 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7261 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7321 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7381 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7441 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7501 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7561 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7621 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7681 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7741 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7801 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7861 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     7921 gaaaagtgcc acctgggtc
//
