LOCUS       sequence-320905-        9206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9206)
  AUTHORS   Backstrom JR, Sheng J, Wang MC, Bernardo-Colon A, Rex TS
  TITLE     Optimization of S. aureus dCas9 and CRISPRi Elements for a Single 
            Adeno-Associated Virus that Targets an Endogenous Gene.
  JOURNAL   Mol Ther Methods Clin Dev. 2020 Sep 6;19:139-148. doi: 
            10.1016/j.omtm.2020.09.001. eCollection 2020 Dec 11.
  PUBMED    33024795
REFERENCE   2  (bases 1 to 9206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2020-09-6"; volume: "19:139-148. doi"; 
            pages: " 10.1016/j.omtm.2020.09.001. eCollection 2020 Dec 11"
COMMENT     Sequence Label: (364) SB KRAB-SadCas9
FEATURES             Location/Qualifiers
     source          1..9206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        284..596
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    292..310
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    328..346
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    399..417
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    435..453
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    470..488
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    506..524
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     569..593
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             683..868
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             4121..4147
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     polyA_signal    4191..4312
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4202..4221)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4256..4275
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             join(4980..5597,5598..5603,5604..5639,5640..5642,5643..5678,
                     5679..5681,5682..5717,5718..5723)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             5730..5786
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5781..6581
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5841..5860)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6451..6470
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6636..6653)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6642..6866
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7192..7209)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7363..7951)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7443..7462)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8122..8913,8914..8982))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8745..8764
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8983..9087)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cagttgaagt cggaagttta catacactta agttggagtc attaaaactc gtttttcaac
       61 tactccacaa atttcttgtt aacaaacaat agttttggca agtcagttag gacatctact
      121 ttgtgcatga cacaagtcat ttttccaaca attgtttaca gacagattat ttcacttata
      181 attcactgta tcacaattcc agtgggtcag aagtttacat acactaagtt cgactcctct
      241 gcagaatgcg gcgatgtttc ggtaaggggt ccgctatcta gacgagttta ctccctatca
      301 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacga tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
      421 tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttat ccctatcagt
      481 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgattagg
      541 cgtgtacggt gggcgcctat aaaagcagag ctcgtttagt gaaccgtcag atcgcctgga
      601 gccaattcca actcttttgc cttataccta ctttccgtac cacttcctac cctcgaaagg
      661 cctctgagaa ttcgccacca tgcggacact agtgaccttc aaggatgtat ttgtggactt
      721 caccagggag gagtggaagc tgctggacac tgctcagcag atcgtgtaca gaaatgtgat
      781 gctggagaac tataagaacc tggtttcctt gggttatcag cttactaagc cagatgtgat
      841 cctccggttg gagaagggag aagagcccgg cggttccggc ggagggtcca tgggcaaacg
      901 gaactacatc ctggggcttg ccattgggat aaccagcgtt ggctacggaa ttattgatta
      961 tgagacacgc gatgtgattg acgccggggt taggctgttc aaagaggcca acgttgaaaa
     1021 caacgaggga agacggagta agcgcggagc aagaagactc aagcgcagac ggagacatcg
     1081 gattcagagg gtgaaaaagc tgctcttcga ttacaatctc ctgaccgatc atagtgagct
     1141 gagcggaatc aacccctacg aggcgcgagt gaaagggctt tcccagaagc tgtccgaaga
     1201 ggagttctcc gccgcgttgc tgcacctggc caaacggagg ggggttcaca atgtaaacga
     1261 agtggaggag gacacgggca atgaacttag tacgaaagaa cagatcagta ggaactctaa
     1321 ggctctcgaa gagaaatacg tcgctgagtt gcagcttgag agactgaaaa aagacggcga
     1381 agtacgcgga tctattaata ggttcaagac ttcagattac gtaaaggaag ccaagcagct
     1441 cctgaaagta cagaaagcgt accatcagct cgatcagagc ttcatcgata cctacataga
     1501 tttgctggag acacggagga catactacga gggcccaggg gaaggatctc cttttgggtg
     1561 gaaggacatc aaggaatggt acgagatgct tatgggacat tgtacatatt ttccggagga
     1621 gctcaggagc gtcaagtacg cctacaatgc cgacctgtac aatgccctca atgacctcaa
     1681 taacctcgtg attaccaggg acgagaacga gaagctggag tactatgaaa agttccagat
     1741 tatcgagaat gtgtttaagc agaagaagaa gccgacactt aagcagattg caaaggaaat
     1801 cctcgtgaat gaggaagata tcaagggata cagagtgaca agtacaggca agcccgagtt
     1861 cacaaatctg aaggtgtacc acgatattaa ggacataacc gcacgaaagg agataatcga
     1921 aaacgctgag ctcctcgatc agatcgcaaa aattcttacc atctaccagt ctagtgagga
     1981 cattcaggag gaactgacta atctgaacag tgagctcacc caagaggaaa ttgagcagat
     2041 ttcaaacctg aaaggctaca ccgggacgca caatctgagc ctcaaagcaa tcaacctcat
     2101 tctggatgaa ctttggcaca caaatgacaa ccaaattgcc atattcaacc gcctgaaact
     2161 ggtgccaaaa aaagtggatc tgtcacagca aaaggaaatc cctacaacct tggttgacga
     2221 ttttattctg tcccccgttg tcaagcggag cttcatccag tcaatcaagg tgatcaatgc
     2281 catcattaaa aaatacggat tgccaaacga tataattatc gagcttgcac gagagaagaa
     2341 ctcaaaggac gcccagaaga tgattaacga aatgcagaag cgcaaccgcc agacaaacga
     2401 acgcatagag gaaattataa gaacaaccgg caaagagaat gccaagtatc tgatcgagaa
     2461 aatcaagctg cacgacatgc aagaaggcaa gtgcctgtac tctctggaag ctatcccact
     2521 cgaagatctg ctgaataatc cattcaatta cgaggtggac gccatcatcc ctagatccgt
     2581 aagctttgac aattccttca ataacaaagt tctggttaaa caggaggaaa attctaaaaa
     2641 agggaaccgg accccgttcc agtacctgag ctccagtgac agcaagatta gctacgagac
     2701 ttttaagaaa catattctga atctggccaa aggcaaaggc aggatcagca agaccaagaa
     2761 ggagtacctc ctcgaagaac gcgacattaa cagatttagt gtgcagaaag atttcatcaa
     2821 ccgaaacctt gtcgatactc ggtacgccac gagaggcctg atgaatctcc tcaggagcta
     2881 cttccgcgtc aataatctgg acgttaaagt caagagcata aatgggggat tcaccagctt
     2941 tctgaggaga aagtggaagt ttaagaagga acgaaacaaa ggatacaagc accatgctga
     3001 ggatgctttg atcatcgcta acgcggactt tatctttaag gaatggaaaa agctggataa
     3061 ggcaaagaaa gtgatggaaa accagatgtt cgaggagaag caggcagagt caatgcctga
     3121 gatcgagaca gagcaggaat acaaggaaat tttcatcacc cctcatcaga ttaaacacat
     3181 aaaggacttc aaagactata aatactctca tagggtggac aaaaaaccca atcgcgagct
     3241 cattaatgac accctgtact caacacggaa ggatgataaa ggtaatacct tgattgtgaa
     3301 taatcttaat ggattgtatg acaaagataa cgacaagctc aagaagctga tcaacaagtc
     3361 tccagagaag ctccttatgt atcaccacga cccacagact tatcagaaat tgaaactgat
     3421 catggagcaa tacggggatg agaagaaccc actctacaaa tattatgagg aaacaggtaa
     3481 ttacctgacc aagtactcca agaaggataa cggaccagtg atcaaaaaga taaagtacta
     3541 tggcaacaaa cttaatgcgc atttggacat aactgacgat taccccaatt ctcgaaacaa
     3601 ggttgtgaag ctctccctga agccttatag atttgacgtg tacctggata atggggttta
     3661 taaattcgtc accgtgaaaa atctggacgt gatcaaaaag gagaactatt atgaagtaaa
     3721 ctcaaagtgc tatgaggagg cgaagaagct gaagaagatc tccaatcagg ccgagttcat
     3781 cgcttccttc tataataacg atctcatcaa gatcaatgga gagctttatc gcgtcattgg
     3841 tgtgaacaat gacttgctga acaggatcga agtcaatatg atagacatta cctaccggga
     3901 gtatctcgaa aacatgaatg ataaacggcc gcctcgcatc atcaagacaa tcgcatctaa
     3961 aactcagtca ataaaaaagt actctaccga tatcctgggg aatctctatg aagtgaagtc
     4021 aaagaagcac ccacaaatca ttaaaaaagg tggatccgca gaagcagcag caaaggaagc
     4081 agcagcaaag gaagcagcag caaaggcagt cgacaccggt ccagcagcta agagagttaa
     4141 actagattag cggccgcatc gtcaggccaa gcttccatcg atagacatga taagatacat
     4201 tgatgagttt ggacaaacca caacaagaat gcagtgaaaa aaatgcttta tttgtgaaat
     4261 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     4321 caattgcatt cattttatgt ttcaggtacc taggcgagac cctgtctcac aaaataaagt
     4381 aagcccggac tgagtgcgga aaggcgggcc tggcgggtct ggtctcccca tgcgggccac
     4441 cagaggccct gcagccttca gtcgcttgaa ggggtaatgg cgcttccact cacaaacatg
     4501 gcggacagag cgtgtgaacg agatgaacag cccctcaaaa atatggccgc cgaggctgga
     4561 cggccgtgcc ccagcagcac cgcctccgcg ccccacgtga tctctcgccg ggcacagcgc
     4621 tgaccgcgga ggtccaaccg gaagaatgtc cggattggac attcggaaga gggcccgcct
     4681 tccctgggga atctctgcgc acgcgcagaa cgcttcgacc aatgaaaaca caggaagccg
     4741 tccgcgcaac cgcgttgcgt cacttctgcc gcccctgttt caaggtatat agccgtagac
     4801 ggaacttcgc ctttctctcg gccttagcgc catttttttg ggtgagtgtt ttttggttcc
     4861 tgcgttggga ttccgtgtac aatccataga catctgacct cggcacttag catcatcaca
     4921 gcaaactaac tgtagccttt ctctctttcc ctgtagaaac ctctgcacct gaggccacca
     4981 tgtctagact ggacaagagc aaagtcataa acggcgctct ggaattactc aatggagtcg
     5041 gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct gggagttgag cagcctaccc
     5101 tgtactggca cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc gagatgctgg
     5161 acaggcatca tacccacttc tgccccctgg aaggcgagtc atggcaagac tttctgcgga
     5221 acaacgccaa gtcattccgc tgtgctctcc tctcacatcg cgacggggct aaagtgcatc
     5281 tcggcacccg cccaacagag aaacagtacg aaaccctgga aaatcagctc gcgttcctgt
     5341 gtcagcaagg cttctccctg gagaacgcac tgtacgctct gtccgccgtg ggccacttta
     5401 cactgggctg cgtattggag gaacaggagc atcaagtagc aaaagaggaa agagagacac
     5461 ctaccaccga ttctatgccc ccacttctga gacaagcaat tgagctgttc gaccggcagg
     5521 gagccgaacc tgccttcctt ttcggcctgg aactaatcat atgtggcctg gagaaacagc
     5581 taaagtgcga aagcggcggg ccggccgacg cccttgacga ttttgactta gacatgctcc
     5641 cagccgatgc ccttgacgac tttgaccttg atatgctgcc tgctgacgct cttgacgatt
     5701 ttgaccttga catgctcccc gggtccggcg ctactaactt cagcctgctg aagcaggctg
     5761 gtgacgtcga ggagaatcct ggtcccatga ttgaacaaga tggattgcac gcaggttctc
     5821 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     5881 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     5941 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     6001 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     6061 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     6121 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     6181 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     6241 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     6301 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccac ggtgatgcct
     6361 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     6421 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     6481 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     6541 agcgcatcgc cttctatcgc cttcttgacg agttcttctg attcgaaggc ctgtcgtgaa
     6601 gcttggggat caattctcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     6661 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     6721 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     6781 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     6841 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct gggcctacta
     6901 gctactcggg accccttacc gaaacatcgc cgcattctgc agaggagtcg agtgtatgta
     6961 aacttctgac ccactgggaa tgtgatgaaa gaaataaaag ctgaaatgaa tcattctctc
     7021 tactattatt ctgatatttc acattcttaa aataaagtgg tgatcctaac tgacctaaga
     7081 cagggaattt ttactaggat taaatgtcag gaattgtgaa aaagtgagtt taaatgtatt
     7141 tggctaaggt gtatgtaaac ttccgacttc aactgtatag ggatccgctt cctcgctcac
     7201 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7261 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7321 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7381 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7441 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7501 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7561 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7621 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7681 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7741 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7801 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7861 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7921 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7981 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8041 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8101 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8161 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8221 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8281 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8341 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8401 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     8461 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8521 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8581 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8641 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     8701 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     8761 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     8821 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     8881 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     8941 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9001 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9061 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgatgcggt
     9121 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg taagcgttaa
     9181 tattttgtta aaattggatc cctata
//
