LOCUS       sequence-320929-        2246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 part - promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Frangedakis E, Guzman-Chavez F, Rebmann M, Markel K, Yu Y, Perraki 
            A, Tse SW, Liu Y, Rever J, Sauret-Gueto S, Goffinet B, Schneider H, 
            Haseloff J
  TITLE     Construction of DNA Tools for Hyperexpression in Marchantia 
            Chloroplasts.
  JOURNAL   ACS Synth Biol. 2021 Jul 16;10(7):1651-1666. doi: 
            10.1021/acssynbio.0c00637. Epub 2021 Jun 7.
  PUBMED    34097383
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00637"; journalName: "ACS Synth Biol"; date: 
            "2021-07-16- 16"; volume: "10"; issue: "7"; pages: "1651-1666"
COMMENT     Sequence Label: PROM5_MprbcL_short_chlo
FEATURES             Location/Qualifiers
     source          1..2246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     228..246
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(250..293)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      515..572
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(596..613)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(767..1355)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(847..866)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1538..1632)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1653..2246,1..66))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2227..2246
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
       61 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
      121 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca actcgagtgc
      181 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
      241 cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt
      301 cggtctctgg agttgattta ataataaaat aactaggttg cattacatat aaaaaacaat
      361 atacaataat aatgttttat tattggaaaa aatttttact taaaaaattt tttatacaaa
      421 agaaaaatta gaaaaaaatt tttatcgagc agacctcata cttgcaagaa tattatttga
      481 tttgtaggga gggacttaat gtgagaccct gcagtccggc aaaaaagggc aaggtgtcac
      541 caccctgccc tttttcttta aaaccgaaaa gattacttcg cgttatgcag gcttcctcgc
      601 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      661 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      721 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc
      781 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      841 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      901 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      961 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1021 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1081 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1141 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1201 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     1261 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     1321 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     1381 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     1441 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     1501 tatatgagta aacttggtct gacagctcga ggcttggatt ctcaccaata aaaaacgccc
     1561 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
     1621 tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag
     1681 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
     1741 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
     1801 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
     1861 acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc
     1921 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
     1981 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
     2041 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc
     2101 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
     2161 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
     2221 cattgagcaa ctgactgaaa tgcctc
//
