LOCUS       sequence-32096-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Lei K, Davis RJ
  TITLE     JNK phosphorylation of Bim-related members of the Bcl2 family 
            induces Bax-dependent apoptosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7.
  PUBMED    12591950
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2003 Mar 4. 100(5):2432-7."
COMMENT     Sequence Label: pCDNA3 T7 BimL(S58A,DE)
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             85..117
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(598..616)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(599..616)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(636..653)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    642..866
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ttagcgactc tctggctact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttatggct agcatgactg gtggacagca aatgggtgga
      121 tccgcaaagc aaccttctga tgtaagttct gagtgtgacc gagaaggtag acaattgcag
      181 cctgcggaga ggcctcccca gctcagacct ggggccccta cctccctaca gacagagcca
      241 caagacagga gcccagcacc catgagttgt gacaaatcaa cacaaacccc agctcctcct
      301 tgccaggcct tcaaccacta tctcagtgca atggcttcca tgaggcaggc tgaacctgca
      361 gatatgcgcc cagagatatg gatcgcccaa gaggatcggc gtgaaggaga cgagtttaac
      421 gcttactatg caaggagggt atttttgaat aattaccaag cagccgaaga ccacccacga
      481 atggttatct tacgactgtt acgttacatt gtccgcctgg tgtggagaat gcattgagaa
      541 ttctgcagat atccatcaca ctggcggccg ctcgagcatg catctagagg gccctattct
      601 atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
      661 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
      721 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
      781 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
      841 tgctggggat gcggtgggct ctatggcttc tga
//
