LOCUS       sequence-32097-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Lei K, Davis RJ
  TITLE     JNK phosphorylation of Bim-related members of the Bcl2 family 
            induces Bax-dependent apoptosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7.
  PUBMED    12591950
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2003 Mar 4. 100(5):2432-7."
COMMENT     Sequence Label: pCDNA3 T7 BimL(S58D,DE)
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             83..115
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(596..614)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(597..614)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(634..651)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    640..864
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ttgcgactct ctggctactg agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc ttatggctag catgactggt ggacagcaaa tgggtggatc
      121 cgcaaagcaa ccttctgatg taagttctga gtgtgaccga gaaggtagac aattgcagca
      181 tgcggagagg cctccccagc tcagacctgg ggcccctacc tccctacaga cagagccaca
      241 agacaggagc ccagcaccca tgagttgtga caaatcaaca caaaccccag atcctccttg
      301 ccaggccttc aaccactatc tcagtgcaat ggcttccatg aggcaggctg aacctgcaga
      361 tatgcgccca gagatatgga tcgcccaaga ggatcggcgt gaaggagacg agtttaacgc
      421 ttactatgca aggagggtat ttttgaataa ttaccaagca gccgaagacc acccacgaat
      481 ggttatctta cgactgttac gttacattgt ccgcctggtg tggagaatgc attgagaatt
      541 ctgcagatat ccatcacact ggcggccgct cgagcatgca tctagagggc cctattctat
      601 agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
      661 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
      721 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
      781 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
      841 ctggggatgc ggtgggctct atggcttctg aggcggaaag aac
//
