LOCUS       sequence-32099-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Lei K, Davis RJ
  TITLE     JNK phosphorylation of Bim-related members of the Bcl2 family 
            induces Bax-dependent apoptosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7.
  PUBMED    12591950
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2003 Mar 4. 100(5):2432-7."
COMMENT     Sequence Label: pCDNA3 Flag BimL(T56A,DE)
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             99..122
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(603..621)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(604..621)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(641..658)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    647..871
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ttagcgactc tctggctact gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttacggcag ccaacatgga ctataaggac gatgatgaca
      121 aaggatccgc aaagcaacct tctgatgtaa gttctgagtg tgaccgagaa ggtagacaat
      181 tgcagcctgc ggagaggcct ccccagctca gacctggggc ccctacctcc ctacagacag
      241 agccacaaga caggagccca gcacccatga gttgtgacaa atcaacacaa gccccaagtc
      301 ctccttgcca ggccttcaac cactatctca gtgcaatggc ttccatgagg caggctgaac
      361 ctgcagatat gcgcccagag atatggatcg cccaagagga tcggcgtgaa ggagacgagt
      421 ttaacgctta ctatgcaagg agggtatttt tgaataatta ccaagcagcc gaagaccacc
      481 cacgaatggt tatcttacga ctgttacgtt acattgtccg cctggtgtgg agaatgcatt
      541 gagaattctg cagatatcca tcacactggc ggccgctcga gcatgcatct agagggccct
      601 attctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt
      661 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
      721 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
      781 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
      841 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
      901 tctagggggt tcccccc
//
