LOCUS       sequence-32100-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Lei K, Davis RJ
  TITLE     JNK phosphorylation of Bim-related members of the Bcl2 family 
            induces Bax-dependent apoptosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7.
  PUBMED    12591950
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2003 Mar 4. 100(5):2432-7."
COMMENT     Sequence Label: pCDNA3 Flag BimL(T56D,DE)
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..77
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        58..76
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             105..128
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(609..627)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(610..627)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(647..664)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    653..877
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gggctttagc gactctctgg ctactagaga acccactgct tactggctta tcgaaattaa
       61 tacgactcac tatagggaga cccaagctta cggcagccaa catggactat aaggacgatg
      121 atgacaaagg atccgcaaag caaccttctg atgtaagttc tgagtgtgac cgagaaggta
      181 gacaattgca gcctgcggag aggcctcccc agctcagacc tggggcccct acctccctac
      241 agacagagcc acaagacagg agcccagcac ccatgagttg tgacaaatca acacaagacc
      301 caagtcctcc ttgccaggcc ttcaaccact atctcagtgc aatggcttcc atgaggcagg
      361 ctgaacctgc agatatgcgc ccagagatat ggatcgccca agaggatcgg cgtgaaggag
      421 acgagtttaa cgcttactat gcaaggaggg tatttttgaa taattaccaa gcagccgaag
      481 accacccacg aatggttatc ttacgactgt tacgttacat tgtccgcctg gtgtggagaa
      541 tgcattgaga attctgcaga tatccatcac actggcggcc gctcgagcat gcatctagag
      601 ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct
      661 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
      721 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
      781 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
      841 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
      901 tggggctct
//
