LOCUS       sequence-32101-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Lei K, Davis RJ
  TITLE     JNK phosphorylation of Bim-related members of the Bcl2 family 
            induces Bax-dependent apoptosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7.
  PUBMED    12591950
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2003 Mar 4. 100(5):2432-7."
COMMENT     Sequence Label: pCDNA3 Flag BimL(S58D,DE)
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             104..127
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(608..626)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(609..626)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(646..663)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    652..876
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gggctttagc gactctctgg ctactgagaa cccactgctt actggcttat cgaaattaat
       61 acgactcact atagggagac ccaagcttac ggcagccaac atggactata aggacgatga
      121 tgacaaagga tccgcaaagc aaccttctga tgtaagttct gagtgtgacc gagaaggtag
      181 acaattgcag cctgcggaga ggcctcccca gctcagacct ggggccccta cctccctaca
      241 gacagagcca caagacagga gcccagcacc catgagttgt gacaaatcaa cacaaacccc
      301 agatcctcct tgccaggcct tcaaccacta tctcagtgca atggcttcca tgaggcaggc
      361 tgaacctgca gatatgcgcc cagagatatg gatcgcccaa gaggatcggc gtgaaggaga
      421 cgagtttaac gcttactatg caaggagggt atttttgaat aattaccaag cagccgaaga
      481 ccacccacga atggttatct tacgactgtt acgttacatt gtccgcctgg tgtggagaat
      541 gcattgagaa ttctgcagat atccatcaca ctggcggccg ctcgagcatg catctagagg
      601 gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt
      661 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
      721 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
      781 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
      841 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
      901 ggggtctagg g
//
