LOCUS       sequence-32104-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Morel C, Carlson SM, White FM, Davis RJ
  TITLE     Mcl-1 integrates the opposing actions of signaling pathways that 
            mediate survival and apoptosis.
  JOURNAL   Mol Cell Biol. 2009 Jul . 29(14):3845-52.
  PUBMED    19433446
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2009 Jul . 29(14):3845-52."
COMMENT     Sequence Label: pBabe-Flag hMcl-1
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        40..58
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      93..102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             102..125
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tggtggtcgt aggaattcta gcgattacgc caagctcgaa attaaccctc actaaaggga
       61 acaaaagctg gagctccacc gcggtggcgg ccgccaccat ggattacaag gatgacgacg
      121 ataaggcccg ggcggatcca ctagtccagt gtggtggaat tgcccttatg tttggcctca
      181 aaagaaacgc ggtaatcgga ctcaacctct actgtggggg ggccggcttg ggggccggca
      241 gcggcggcgc cacccgcccg ggagggcgac ttttggctac ggagaaggag gcctcggccc
      301 ggcgagagat agggggaggg gaggccggcg cggtgattgg cggaagcgcc ggcgcaagcc
      361 ccccgtccac cctcacgcca gactcccgga gggtcgcgcg gccgccgccc attggcgccg
      421 aggtccccga cgtcaccgcg acccccgcga ggctgctttt cttcgcgccc acccgccgcg
      481 cggcgccgct tgaggagatg gaagccccgg ccgctgacgc catcatgtcg cccgaagagg
      541 agctggacgg gtacgagccg gagcctctcg ggaagcggcc ggctgtcctg ccgctgctgg
      601 agttggtcgg ggaatctggt aataacacca gtacggacgg gtcactaccc tcgacgccgc
      661 cgccagcaga ggaggaggac gagttgtacc ggcagtcgct ggagattatc tctcggtacc
      721 ttcgggagca ggccaccggc gccaaggaca caaagccaat gggcaggtct ggggccacca
      781 gcangaaggc gctggagacc ttac
//
