LOCUS       sequence-32105-e         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Morel C, Carlson SM, White FM, Davis RJ
  TITLE     Mcl-1 integrates the opposing actions of signaling pathways that 
            mediate survival and apoptosis.
  JOURNAL   Mol Cell Biol. 2009 Jul . 29(14):3845-52.
  PUBMED    19433446
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2009 Jul . 29(14):3845-52."
COMMENT     Sequence Label: pBabe-Flag hMcl-1(S159A)
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        28..46
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      81..90
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             90..113
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gaattctagc gattacgcca agctcgaaat taaccctcac taaagggaac aaaagctgga
       61 gctccaccgc ggtggcggcc gccaccatgg attacaagga tgacgacgat aaggcccggg
      121 cggatccact agtccagtgt ggtggaattg cccttatgtt tggcctcaaa agaaacgcgg
      181 taatcggact caacctctac tgtggggggg ccggcttggg ggccggcagc ggcggcgcca
      241 cccgcccggg agggcgactt ttggctacgg agaaggaggc ctcggcccgg cgagagatag
      301 ggggagggga ggccggcgcg gtgattggcg gaagcgccgg cgcaagcccc ccgtccaccc
      361 tcacgccaga ctcccggagg gtcgcgcggc cgccgcccat tggcgccgag gtccccgacg
      421 tcaccgcgac ccccgcgagg ctgcttttct tcgcgcccac ccgccgcgcg gcgccgcttg
      481 aggagatgga agccccggcc gctgacgcca tcatgtcgcc cgaagaggag ctggacgggt
      541 acgagccgga gcctctcggg aagcggccgg ctgtcctgcc gctgctggag ttggtcgggg
      601 aatctggtaa taacaccagt acggacgggg cactaccctc gacgccgccg ccagcagagg
      661 aggaggacga gttgtaccgg cagtcgctgg agattatctc tcggtacctt cgggagcagg
      721 ccaccggcgc caaggacaca aagccaatgg gcaggtctgg ggccacca
//
