LOCUS       sequence-32106-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Morel C, Carlson SM, White FM, Davis RJ
  TITLE     Mcl-1 integrates the opposing actions of signaling pathways that 
            mediate survival and apoptosis.
  JOURNAL   Mol Cell Biol. 2009 Jul . 29(14):3845-52.
  PUBMED    19433446
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2009 Jul . 29(14):3845-52."
COMMENT     Sequence Label: pBabe-Flag hMcl-1(T163A)
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        23..41
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      76..85
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             85..108
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctagcgatta cgccaagctc gaaattaacc ctcactaaag ggaacaaaag ctggagctcc
       61 accgcggtgg cggccgccac catggattac aaggatgacg acgataaggc ccgggcggat
      121 ccactagtcc agtgtggtgg aattgccctt atgtttggcc tcaaaagaaa cgcggtaatc
      181 ggactcaacc tctactgtgg gggggccggc ttgggggccg gcagcggcgg cgccacccgc
      241 ccgggagggc gacttttggc tacggagaag gaggcctcgg cccggcgaga gataggggga
      301 ggggaggccg gcgcggtgat tggcggaagc gccggcgcaa gccccccgtc caccctcacg
      361 ccagactccc ggagggtcgc gcggccgccg cccattggcg ccgaggtccc cgacgtcacc
      421 gcgacccccg cgaggctgct tttcttcgcg cccacccgcc gcgcggcgcc gcttgaggag
      481 atggaagccc cggccgctga cgccatcatg tcgcccgaag aggagctgga cgggtacgag
      541 ccggagcctc tcgggaagcg gccggctgtc ctgccgctgc tggagttggt cggggaatct
      601 ggtaataaca ccagtacgga cgggtcacta ccctcggcgc cgccgccagc agaggaggag
      661 gacgagttgt accggcagtc gctggagatt atctctcggt accttcggga gcaggccacc
      721 ggcgccaagg acacaaagcc aatgggcagg tctggggcca ccagcaggaa ggcgctggag
      781 accttacgac gggttgggga tggcgtgcan cgcaaccacn agacggcctt ccaaggcatg
      841 cttcggaaac tggacatcaa aa
//
