LOCUS       sequence-32125-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  TITLE     pCMV-Flag-hMcl-1
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCMV-Flag-hMcl-1(T163A)
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        47..65
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             109..132
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tggtttagtg aaccgtcaga tccgctagcg attacgccaa gctcgaaatt aaccctcact
       61 aaagggaaca aaagctggag ctccaccgcg gtggcggccg ccaccatgga ttacaaggat
      121 gacgacgata aggcccgggc ggatccacta gtccagtgtg gtggaattgc ccttatgttt
      181 ggcctcaaaa gaaacgcggt aatcggactc aacctctact gtgggggggc cggcttgggg
      241 gccggcagcg gcggcgccac ccgcccggga gggcgacttt tggctacgga gaaggaggcc
      301 tcggcccggc gagagatagg gggaggggag gccggcgcgg tgattggcgg aagcgccggc
      361 gcaagccccc cgtccaccct cacgccagac tcccggaggg tcgcgcggcc gccgcccatt
      421 ggcgccgagg tccccgacgt caccgcgacc cccgcgaggc tgcttttctt cgcgcccacc
      481 cgccgcgcgg cgccgcttga ggagatggaa gccccggccg ctgacgccat catgtcgccc
      541 gaagaggagc tggacgggta cgagccggag cctctcggga agcggccggc tgtcctgccg
      601 ctgctggagt tggtcgggga atctggtaat aacaccagta cggacgggtc actaccctcg
      661 gcgccgccgc cagcagagga ggaggacgag ttgtaccggc agtcgctgga gattatctct
      721 cggtaccttc gggagcaggc caccggcgcc aaggacacaa agccaatggg caggtctggg
      781 gccaccagca ggaaggcgct ggagacctta cgacgggttg gggatggcgt gca
//
